Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01884 and MIT1002_01885 overlap by 4 nucleotides MIT1002_01885 and MIT1002_01886 are separated by 130 nucleotides
MIT1002_01884: MIT1002_01884 - Glutamate 5-kinase, at 2,096,923 to 2,098,032
_01884
MIT1002_01885: MIT1002_01885 - Gamma-glutamyl phosphate reductase, at 2,098,029 to 2,099,276
_01885
MIT1002_01886: MIT1002_01886 - Mechanosensitive ion channel, at 2,099,407 to 2,100,357
_01886
Position (kb)
2098
2099
2100 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2097.072 kb on + strand, within MIT1002_01884 at 2097.087 kb on - strand, within MIT1002_01884 at 2097.211 kb on + strand, within MIT1002_01884 at 2097.228 kb on - strand, within MIT1002_01884 at 2097.409 kb on + strand, within MIT1002_01884 at 2097.427 kb on - strand, within MIT1002_01884 at 2097.434 kb on - strand, within MIT1002_01884 at 2097.441 kb on - strand, within MIT1002_01884 at 2097.542 kb on - strand, within MIT1002_01884 at 2097.578 kb on - strand, within MIT1002_01884 at 2097.602 kb on + strand, within MIT1002_01884 at 2097.689 kb on - strand, within MIT1002_01884 at 2097.755 kb on - strand, within MIT1002_01884 at 2097.755 kb on - strand, within MIT1002_01884 at 2097.799 kb on + strand, within MIT1002_01884 at 2097.805 kb on + strand, within MIT1002_01884 at 2097.840 kb on + strand, within MIT1002_01884 at 2097.865 kb on - strand, within MIT1002_01884 at 2097.881 kb on + strand, within MIT1002_01884 at 2097.881 kb on + strand, within MIT1002_01884 at 2097.881 kb on + strand, within MIT1002_01884 at 2097.881 kb on + strand, within MIT1002_01884 at 2097.889 kb on - strand, within MIT1002_01884 at 2098.000 kb on + strand at 2098.113 kb on + strand at 2098.205 kb on + strand, within MIT1002_01885 at 2098.329 kb on + strand, within MIT1002_01885 at 2098.329 kb on + strand, within MIT1002_01885 at 2098.367 kb on + strand, within MIT1002_01885 at 2098.375 kb on - strand, within MIT1002_01885 at 2098.408 kb on - strand, within MIT1002_01885 at 2098.408 kb on - strand, within MIT1002_01885 at 2098.489 kb on - strand, within MIT1002_01885 at 2098.553 kb on - strand, within MIT1002_01885 at 2098.591 kb on + strand, within MIT1002_01885 at 2098.592 kb on - strand, within MIT1002_01885 at 2098.686 kb on + strand, within MIT1002_01885 at 2098.702 kb on - strand, within MIT1002_01885 at 2098.702 kb on - strand, within MIT1002_01885 at 2098.720 kb on - strand, within MIT1002_01885 at 2098.780 kb on + strand, within MIT1002_01885 at 2098.790 kb on + strand, within MIT1002_01885 at 2098.790 kb on + strand, within MIT1002_01885 at 2098.807 kb on + strand, within MIT1002_01885 at 2098.812 kb on + strand, within MIT1002_01885 at 2098.815 kb on - strand, within MIT1002_01885 at 2098.820 kb on - strand, within MIT1002_01885 at 2098.884 kb on - strand, within MIT1002_01885 at 2098.921 kb on + strand, within MIT1002_01885 at 2098.982 kb on + strand, within MIT1002_01885 at 2099.027 kb on + strand, within MIT1002_01885 at 2099.047 kb on - strand, within MIT1002_01885 at 2099.105 kb on - strand, within MIT1002_01885 at 2099.185 kb on + strand at 2099.200 kb on - strand at 2099.214 kb on + strand at 2099.214 kb on + strand at 2099.222 kb on - strand at 2099.222 kb on - strand at 2099.423 kb on + strand at 2099.423 kb on + strand at 2099.544 kb on + strand, within MIT1002_01886 at 2099.596 kb on - strand, within MIT1002_01886 at 2099.610 kb on - strand, within MIT1002_01886 at 2099.667 kb on + strand, within MIT1002_01886 at 2099.685 kb on - strand, within MIT1002_01886 at 2099.719 kb on - strand, within MIT1002_01886 at 2099.760 kb on + strand, within MIT1002_01886 at 2099.760 kb on + strand, within MIT1002_01886 at 2099.765 kb on + strand, within MIT1002_01886 at 2099.765 kb on + strand, within MIT1002_01886 at 2099.768 kb on - strand, within MIT1002_01886 at 2099.773 kb on + strand, within MIT1002_01886 at 2099.773 kb on - strand, within MIT1002_01886 at 2099.773 kb on - strand, within MIT1002_01886 at 2099.823 kb on + strand, within MIT1002_01886 at 2099.831 kb on - strand, within MIT1002_01886 at 2099.831 kb on - strand, within MIT1002_01886 at 2099.851 kb on + strand, within MIT1002_01886 at 2099.889 kb on + strand, within MIT1002_01886 at 2099.908 kb on + strand, within MIT1002_01886 at 2099.990 kb on + strand, within MIT1002_01886 at 2100.058 kb on - strand, within MIT1002_01886 at 2100.063 kb on - strand, within MIT1002_01886 at 2100.121 kb on - strand, within MIT1002_01886 at 2100.190 kb on + strand, within MIT1002_01886 at 2100.224 kb on + strand, within MIT1002_01886 at 2100.224 kb on + strand, within MIT1002_01886 at 2100.224 kb on + strand, within MIT1002_01886 at 2100.232 kb on - strand, within MIT1002_01886 at 2100.232 kb on - strand, within MIT1002_01886
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 2,097,072 + MIT1002_01884 0.13 +0.8 2,097,087 - MIT1002_01884 0.15 -0.5 2,097,211 + MIT1002_01884 0.26 -1.2 2,097,228 - MIT1002_01884 0.27 -0.6 2,097,409 + MIT1002_01884 0.44 -0.3 2,097,427 - MIT1002_01884 0.45 -1.2 2,097,434 - MIT1002_01884 0.46 -0.8 2,097,441 - MIT1002_01884 0.47 -3.1 2,097,542 - MIT1002_01884 0.56 +0.6 2,097,578 - MIT1002_01884 0.59 -0.8 2,097,602 + MIT1002_01884 0.61 -0.2 2,097,689 - MIT1002_01884 0.69 -5.0 2,097,755 - MIT1002_01884 0.75 -1.0 2,097,755 - MIT1002_01884 0.75 -1.6 2,097,799 + MIT1002_01884 0.79 -3.0 2,097,805 + MIT1002_01884 0.79 -0.2 2,097,840 + MIT1002_01884 0.83 -1.2 2,097,865 - MIT1002_01884 0.85 -2.5 2,097,881 + MIT1002_01884 0.86 -1.0 2,097,881 + MIT1002_01884 0.86 -0.3 2,097,881 + MIT1002_01884 0.86 -0.0 2,097,881 + MIT1002_01884 0.86 +1.0 2,097,889 - MIT1002_01884 0.87 -3.2 2,098,000 + -2.6 2,098,113 + -2.9 2,098,205 + MIT1002_01885 0.14 -3.9 2,098,329 + MIT1002_01885 0.24 -3.0 2,098,329 + MIT1002_01885 0.24 -2.3 2,098,367 + MIT1002_01885 0.27 -2.7 2,098,375 - MIT1002_01885 0.28 -3.3 2,098,408 - MIT1002_01885 0.30 -2.3 2,098,408 - MIT1002_01885 0.30 -1.8 2,098,489 - MIT1002_01885 0.37 -4.5 2,098,553 - MIT1002_01885 0.42 -0.9 2,098,591 + MIT1002_01885 0.45 -2.1 2,098,592 - MIT1002_01885 0.45 -6.0 2,098,686 + MIT1002_01885 0.53 -2.0 2,098,702 - MIT1002_01885 0.54 -0.9 2,098,702 - MIT1002_01885 0.54 -2.0 2,098,720 - MIT1002_01885 0.55 -1.3 2,098,780 + MIT1002_01885 0.60 -2.7 2,098,790 + MIT1002_01885 0.61 -2.3 2,098,790 + MIT1002_01885 0.61 -4.1 2,098,807 + MIT1002_01885 0.62 -5.1 2,098,812 + MIT1002_01885 0.63 -2.6 2,098,815 - MIT1002_01885 0.63 -1.4 2,098,820 - MIT1002_01885 0.63 -3.7 2,098,884 - MIT1002_01885 0.69 -0.7 2,098,921 + MIT1002_01885 0.71 -2.8 2,098,982 + MIT1002_01885 0.76 -2.2 2,099,027 + MIT1002_01885 0.80 -2.7 2,099,047 - MIT1002_01885 0.82 -4.8 2,099,105 - MIT1002_01885 0.86 -0.7 2,099,185 + -6.2 2,099,200 - -3.2 2,099,214 + -4.0 2,099,214 + -0.9 2,099,222 - -3.3 2,099,222 - -2.6 2,099,423 + -0.4 2,099,423 + +1.6 2,099,544 + MIT1002_01886 0.14 -0.2 2,099,596 - MIT1002_01886 0.20 +0.3 2,099,610 - MIT1002_01886 0.21 -0.5 2,099,667 + MIT1002_01886 0.27 -0.1 2,099,685 - MIT1002_01886 0.29 -0.5 2,099,719 - MIT1002_01886 0.33 -0.4 2,099,760 + MIT1002_01886 0.37 +0.2 2,099,760 + MIT1002_01886 0.37 -0.1 2,099,765 + MIT1002_01886 0.38 -0.1 2,099,765 + MIT1002_01886 0.38 -1.9 2,099,768 - MIT1002_01886 0.38 -1.2 2,099,773 + MIT1002_01886 0.38 +1.1 2,099,773 - MIT1002_01886 0.38 +0.6 2,099,773 - MIT1002_01886 0.38 +0.2 2,099,823 + MIT1002_01886 0.44 -0.2 2,099,831 - MIT1002_01886 0.45 +0.0 2,099,831 - MIT1002_01886 0.45 -0.6 2,099,851 + MIT1002_01886 0.47 -0.3 2,099,889 + MIT1002_01886 0.51 -2.2 2,099,908 + MIT1002_01886 0.53 +0.7 2,099,990 + MIT1002_01886 0.61 -0.2 2,100,058 - MIT1002_01886 0.68 -1.2 2,100,063 - MIT1002_01886 0.69 +0.3 2,100,121 - MIT1002_01886 0.75 -0.2 2,100,190 + MIT1002_01886 0.82 +0.2 2,100,224 + MIT1002_01886 0.86 -0.3 2,100,224 + MIT1002_01886 0.86 -1.7 2,100,224 + MIT1002_01886 0.86 +0.3 2,100,232 - MIT1002_01886 0.87 -3.0 2,100,232 - MIT1002_01886 0.87 -0.8
Or see this region's nucleotide sequence