Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01851 and MIT1002_01852 are separated by 30 nucleotides MIT1002_01852 and MIT1002_01853 are separated by 59 nucleotides MIT1002_01853 and MIT1002_01854 are separated by 17 nucleotides MIT1002_01854 and MIT1002_01855 are separated by 49 nucleotides
MIT1002_01851: MIT1002_01851 - Sporulation-specific penicillin-binding protein, at 2,060,780 to 2,062,693
_01851
MIT1002_01852: MIT1002_01852 - Ribosomal RNA large subunit methyltransferase H, at 2,062,724 to 2,063,251
_01852
MIT1002_01853: MIT1002_01853 - Ribosomal silencing factor RsfS, at 2,063,311 to 2,063,628
_01853
MIT1002_01854: MIT1002_01854 - Nicotinate-nucleotide adenylyltransferase, at 2,063,646 to 2,064,311
_01854
MIT1002_01855: MIT1002_01855 - DNA polymerase III subunit delta, at 2,064,361 to 2,065,389
_01855
Position (kb) 2063
2064 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 2062.908 kb on - strand, within MIT1002_01852 at 2063.526 kb on - strand, within MIT1002_01853 at 2063.573 kb on - strand, within MIT1002_01853 at 2063.631 kb on - strand at 2064.398 kb on - strand at 2064.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 2,062,908 - MIT1002_01852 0.35 +0.9 2,063,526 - MIT1002_01853 0.68 -1.0 2,063,573 - MIT1002_01853 0.82 -2.1 2,063,631 - +1.8 2,064,398 - -3.4 2,064,398 - +0.3
Or see this region's nucleotide sequence