Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01378 and MIT1002_01379 are separated by 38 nucleotides MIT1002_01379 and MIT1002_01380 overlap by 1 nucleotides
MIT1002_01378: MIT1002_01378 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 1,528,926 to 1,530,251
_01378
MIT1002_01379: MIT1002_01379 - Biotin synthase, at 1,530,290 to 1,531,414
_01379
MIT1002_01380: MIT1002_01380 - 8-amino-7-oxononanoate synthase, at 1,531,414 to 1,532,775
_01380
Position (kb)
1530
1531
1532 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1529.297 kb on + strand, within MIT1002_01378 at 1529.317 kb on - strand, within MIT1002_01378 at 1529.324 kb on + strand, within MIT1002_01378 at 1529.324 kb on + strand, within MIT1002_01378 at 1529.329 kb on + strand, within MIT1002_01378 at 1529.338 kb on + strand, within MIT1002_01378 at 1529.356 kb on - strand, within MIT1002_01378 at 1529.356 kb on - strand, within MIT1002_01378 at 1529.395 kb on + strand, within MIT1002_01378 at 1529.424 kb on + strand, within MIT1002_01378 at 1529.424 kb on + strand, within MIT1002_01378 at 1529.439 kb on + strand, within MIT1002_01378 at 1529.439 kb on + strand, within MIT1002_01378 at 1529.447 kb on - strand, within MIT1002_01378 at 1529.447 kb on - strand, within MIT1002_01378 at 1529.495 kb on + strand, within MIT1002_01378 at 1529.540 kb on + strand, within MIT1002_01378 at 1529.555 kb on - strand, within MIT1002_01378 at 1529.561 kb on - strand, within MIT1002_01378 at 1529.594 kb on - strand, within MIT1002_01378 at 1529.735 kb on + strand, within MIT1002_01378 at 1529.813 kb on - strand, within MIT1002_01378 at 1529.828 kb on - strand, within MIT1002_01378 at 1529.868 kb on - strand, within MIT1002_01378 at 1529.872 kb on + strand, within MIT1002_01378 at 1529.886 kb on + strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.945 kb on + strand, within MIT1002_01378 at 1530.003 kb on + strand, within MIT1002_01378 at 1530.008 kb on + strand, within MIT1002_01378 at 1530.013 kb on + strand, within MIT1002_01378 at 1530.016 kb on - strand, within MIT1002_01378 at 1530.017 kb on + strand, within MIT1002_01378 at 1530.084 kb on + strand, within MIT1002_01378 at 1530.084 kb on + strand, within MIT1002_01378 at 1530.144 kb on + strand at 1530.145 kb on + strand at 1530.145 kb on + strand at 1530.145 kb on + strand at 1530.145 kb on + strand at 1530.151 kb on - strand at 1530.151 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.253 kb on + strand at 1530.253 kb on + strand at 1530.253 kb on + strand at 1530.261 kb on - strand at 1530.377 kb on + strand at 1530.525 kb on - strand, within MIT1002_01379 at 1530.660 kb on - strand, within MIT1002_01379 at 1530.695 kb on + strand, within MIT1002_01379 at 1530.695 kb on - strand, within MIT1002_01379 at 1530.710 kb on + strand, within MIT1002_01379 at 1530.710 kb on - strand, within MIT1002_01379 at 1530.727 kb on - strand, within MIT1002_01379 at 1530.782 kb on + strand, within MIT1002_01379 at 1530.782 kb on + strand, within MIT1002_01379 at 1530.830 kb on + strand, within MIT1002_01379 at 1530.833 kb on - strand, within MIT1002_01379 at 1530.838 kb on - strand, within MIT1002_01379 at 1530.880 kb on - strand, within MIT1002_01379 at 1530.909 kb on + strand, within MIT1002_01379 at 1530.932 kb on + strand, within MIT1002_01379 at 1530.932 kb on + strand, within MIT1002_01379 at 1530.957 kb on + strand, within MIT1002_01379 at 1530.957 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1531.031 kb on - strand, within MIT1002_01379 at 1531.031 kb on - strand, within MIT1002_01379 at 1531.097 kb on - strand, within MIT1002_01379 at 1531.102 kb on - strand, within MIT1002_01379 at 1531.121 kb on + strand, within MIT1002_01379 at 1531.198 kb on - strand, within MIT1002_01379 at 1531.270 kb on + strand, within MIT1002_01379 at 1531.271 kb on - strand, within MIT1002_01379 at 1531.372 kb on + strand at 1531.372 kb on + strand at 1531.372 kb on + strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.380 kb on - strand at 1531.386 kb on + strand at 1531.404 kb on - strand at 1531.417 kb on + strand at 1531.456 kb on + strand at 1531.554 kb on + strand, within MIT1002_01380 at 1531.572 kb on + strand, within MIT1002_01380 at 1531.628 kb on + strand, within MIT1002_01380 at 1531.631 kb on + strand, within MIT1002_01380 at 1531.631 kb on - strand, within MIT1002_01380 at 1531.670 kb on - strand, within MIT1002_01380 at 1531.730 kb on - strand, within MIT1002_01380 at 1531.778 kb on + strand, within MIT1002_01380 at 1531.864 kb on + strand, within MIT1002_01380 at 1531.868 kb on - strand, within MIT1002_01380 at 1531.868 kb on - strand, within MIT1002_01380 at 1531.874 kb on + strand, within MIT1002_01380 at 1531.874 kb on + strand, within MIT1002_01380 at 1531.882 kb on - strand, within MIT1002_01380 at 1531.882 kb on - strand, within MIT1002_01380 at 1532.054 kb on + strand, within MIT1002_01380 at 1532.108 kb on + strand, within MIT1002_01380 at 1532.108 kb on + strand, within MIT1002_01380 at 1532.116 kb on - strand, within MIT1002_01380 at 1532.116 kb on - strand, within MIT1002_01380 at 1532.116 kb on - strand, within MIT1002_01380 at 1532.144 kb on - strand, within MIT1002_01380 at 1532.149 kb on + strand, within MIT1002_01380 at 1532.164 kb on - strand, within MIT1002_01380 at 1532.164 kb on - strand, within MIT1002_01380 at 1532.174 kb on + strand, within MIT1002_01380 at 1532.203 kb on + strand, within MIT1002_01380 at 1532.222 kb on + strand, within MIT1002_01380 at 1532.322 kb on + strand, within MIT1002_01380 at 1532.325 kb on + strand, within MIT1002_01380 at 1532.330 kb on + strand, within MIT1002_01380 at 1532.330 kb on + strand, within MIT1002_01380 at 1532.330 kb on - strand, within MIT1002_01380 at 1532.338 kb on - strand, within MIT1002_01380 at 1532.338 kb on - strand, within MIT1002_01380 at 1532.338 kb on - strand, within MIT1002_01380 at 1532.358 kb on - strand, within MIT1002_01380 at 1532.358 kb on - strand, within MIT1002_01380
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 1,529,297 + MIT1002_01378 0.28 -3.1 1,529,317 - MIT1002_01378 0.29 -3.0 1,529,324 + MIT1002_01378 0.30 -1.9 1,529,324 + MIT1002_01378 0.30 +0.4 1,529,329 + MIT1002_01378 0.30 -2.3 1,529,338 + MIT1002_01378 0.31 -2.5 1,529,356 - MIT1002_01378 0.32 -2.3 1,529,356 - MIT1002_01378 0.32 -1.3 1,529,395 + MIT1002_01378 0.35 -2.6 1,529,424 + MIT1002_01378 0.38 -2.6 1,529,424 + MIT1002_01378 0.38 -0.6 1,529,439 + MIT1002_01378 0.39 -2.7 1,529,439 + MIT1002_01378 0.39 -2.6 1,529,447 - MIT1002_01378 0.39 -2.1 1,529,447 - MIT1002_01378 0.39 -2.8 1,529,495 + MIT1002_01378 0.43 -2.0 1,529,540 + MIT1002_01378 0.46 -2.8 1,529,555 - MIT1002_01378 0.47 -2.1 1,529,561 - MIT1002_01378 0.48 -4.2 1,529,594 - MIT1002_01378 0.50 -2.5 1,529,735 + MIT1002_01378 0.61 -2.5 1,529,813 - MIT1002_01378 0.67 -2.3 1,529,828 - MIT1002_01378 0.68 +0.4 1,529,868 - MIT1002_01378 0.71 -2.2 1,529,872 + MIT1002_01378 0.71 -1.7 1,529,886 + MIT1002_01378 0.72 -3.2 1,529,894 - MIT1002_01378 0.73 -0.4 1,529,894 - MIT1002_01378 0.73 -2.3 1,529,894 - MIT1002_01378 0.73 -0.1 1,529,894 - MIT1002_01378 0.73 -3.4 1,529,945 + MIT1002_01378 0.77 -4.8 1,530,003 + MIT1002_01378 0.81 -5.7 1,530,008 + MIT1002_01378 0.82 -3.8 1,530,013 + MIT1002_01378 0.82 -0.1 1,530,016 - MIT1002_01378 0.82 -6.5 1,530,017 + MIT1002_01378 0.82 -2.8 1,530,084 + MIT1002_01378 0.87 -2.6 1,530,084 + MIT1002_01378 0.87 -1.6 1,530,144 + -4.3 1,530,145 + -6.3 1,530,145 + -0.4 1,530,145 + -4.4 1,530,145 + -3.6 1,530,151 - -2.8 1,530,151 - -2.2 1,530,153 - -2.9 1,530,153 - -1.6 1,530,153 - -3.9 1,530,153 - -0.7 1,530,153 - -1.5 1,530,253 + -2.0 1,530,253 + -2.5 1,530,253 + -5.6 1,530,261 - -1.9 1,530,377 + -3.9 1,530,525 - MIT1002_01379 0.21 -3.1 1,530,660 - MIT1002_01379 0.33 -0.9 1,530,695 + MIT1002_01379 0.36 -1.4 1,530,695 - MIT1002_01379 0.36 -3.3 1,530,710 + MIT1002_01379 0.37 -4.0 1,530,710 - MIT1002_01379 0.37 -3.3 1,530,727 - MIT1002_01379 0.39 -0.7 1,530,782 + MIT1002_01379 0.44 -3.6 1,530,782 + MIT1002_01379 0.44 -2.4 1,530,830 + MIT1002_01379 0.48 -3.5 1,530,833 - MIT1002_01379 0.48 -3.6 1,530,838 - MIT1002_01379 0.49 -3.8 1,530,880 - MIT1002_01379 0.52 -3.7 1,530,909 + MIT1002_01379 0.55 -2.1 1,530,932 + MIT1002_01379 0.57 -1.9 1,530,932 + MIT1002_01379 0.57 -3.4 1,530,957 + MIT1002_01379 0.59 -1.6 1,530,957 + MIT1002_01379 0.59 -1.4 1,530,967 + MIT1002_01379 0.60 -0.9 1,530,967 + MIT1002_01379 0.60 -6.9 1,530,967 + MIT1002_01379 0.60 -2.4 1,530,967 + MIT1002_01379 0.60 -2.6 1,530,967 + MIT1002_01379 0.60 -6.8 1,530,967 + MIT1002_01379 0.60 -3.2 1,530,975 - MIT1002_01379 0.61 -7.7 1,530,975 - MIT1002_01379 0.61 -1.1 1,530,975 - MIT1002_01379 0.61 -3.5 1,530,975 - MIT1002_01379 0.61 -2.5 1,530,975 - MIT1002_01379 0.61 -2.7 1,531,031 - MIT1002_01379 0.66 -3.1 1,531,031 - MIT1002_01379 0.66 -1.8 1,531,097 - MIT1002_01379 0.72 -2.7 1,531,102 - MIT1002_01379 0.72 -0.6 1,531,121 + MIT1002_01379 0.74 -2.3 1,531,198 - MIT1002_01379 0.81 -2.4 1,531,270 + MIT1002_01379 0.87 -1.0 1,531,271 - MIT1002_01379 0.87 -2.0 1,531,372 + +0.5 1,531,372 + -0.4 1,531,372 + -0.9 1,531,380 - -4.2 1,531,380 - -3.7 1,531,380 - -3.9 1,531,380 - -2.8 1,531,380 - -3.5 1,531,380 - -3.5 1,531,380 - -1.0 1,531,380 - -4.2 1,531,386 + +1.4 1,531,404 - -1.4 1,531,417 + -2.1 1,531,456 + -4.4 1,531,554 + MIT1002_01380 0.10 -3.3 1,531,572 + MIT1002_01380 0.12 -3.6 1,531,628 + MIT1002_01380 0.16 -1.0 1,531,631 + MIT1002_01380 0.16 -4.6 1,531,631 - MIT1002_01380 0.16 -5.0 1,531,670 - MIT1002_01380 0.19 -1.4 1,531,730 - MIT1002_01380 0.23 -4.2 1,531,778 + MIT1002_01380 0.27 -1.1 1,531,864 + MIT1002_01380 0.33 -0.4 1,531,868 - MIT1002_01380 0.33 -1.6 1,531,868 - MIT1002_01380 0.33 -2.4 1,531,874 + MIT1002_01380 0.34 -0.6 1,531,874 + MIT1002_01380 0.34 -2.4 1,531,882 - MIT1002_01380 0.34 -0.4 1,531,882 - MIT1002_01380 0.34 -2.4 1,532,054 + MIT1002_01380 0.47 -1.8 1,532,108 + MIT1002_01380 0.51 -3.5 1,532,108 + MIT1002_01380 0.51 -2.9 1,532,116 - MIT1002_01380 0.52 -4.0 1,532,116 - MIT1002_01380 0.52 -2.5 1,532,116 - MIT1002_01380 0.52 -3.2 1,532,144 - MIT1002_01380 0.54 -2.8 1,532,149 + MIT1002_01380 0.54 -1.3 1,532,164 - MIT1002_01380 0.55 -2.9 1,532,164 - MIT1002_01380 0.55 -3.3 1,532,174 + MIT1002_01380 0.56 -2.1 1,532,203 + MIT1002_01380 0.58 +0.1 1,532,222 + MIT1002_01380 0.59 -0.7 1,532,322 + MIT1002_01380 0.67 -2.2 1,532,325 + MIT1002_01380 0.67 -2.4 1,532,330 + MIT1002_01380 0.67 -0.4 1,532,330 + MIT1002_01380 0.67 -3.0 1,532,330 - MIT1002_01380 0.67 -1.6 1,532,338 - MIT1002_01380 0.68 -2.1 1,532,338 - MIT1002_01380 0.68 -1.6 1,532,338 - MIT1002_01380 0.68 -2.3 1,532,358 - MIT1002_01380 0.69 -2.7 1,532,358 - MIT1002_01380 0.69 -2.9
Or see this region's nucleotide sequence