Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01377 and MIT1002_01378 are separated by 172 nucleotides MIT1002_01378 and MIT1002_01379 are separated by 38 nucleotides
MIT1002_01377: MIT1002_01377 - hypothetical protein, at 1,528,232 to 1,528,753
_01377
MIT1002_01378: MIT1002_01378 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 1,528,926 to 1,530,251
_01378
MIT1002_01379: MIT1002_01379 - Biotin synthase, at 1,530,290 to 1,531,414
_01379
Position (kb)
1528
1529
1530
1531 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1527.936 kb on - strand at 1527.946 kb on - strand at 1527.985 kb on - strand at 1528.066 kb on - strand at 1528.099 kb on - strand at 1528.154 kb on - strand at 1528.154 kb on - strand at 1528.167 kb on - strand at 1528.217 kb on + strand at 1528.262 kb on - strand at 1528.356 kb on + strand, within MIT1002_01377 at 1528.374 kb on - strand, within MIT1002_01377 at 1528.374 kb on - strand, within MIT1002_01377 at 1528.448 kb on + strand, within MIT1002_01377 at 1528.488 kb on - strand, within MIT1002_01377 at 1528.488 kb on - strand, within MIT1002_01377 at 1528.505 kb on + strand, within MIT1002_01377 at 1528.562 kb on + strand, within MIT1002_01377 at 1528.582 kb on - strand, within MIT1002_01377 at 1528.755 kb on - strand at 1528.755 kb on - strand at 1528.830 kb on - strand at 1528.839 kb on + strand at 1528.846 kb on + strand at 1528.847 kb on - strand at 1528.873 kb on + strand at 1528.970 kb on + strand at 1529.029 kb on - strand at 1529.077 kb on + strand, within MIT1002_01378 at 1529.114 kb on + strand, within MIT1002_01378 at 1529.115 kb on - strand, within MIT1002_01378 at 1529.120 kb on + strand, within MIT1002_01378 at 1529.128 kb on - strand, within MIT1002_01378 at 1529.247 kb on + strand, within MIT1002_01378 at 1529.247 kb on + strand, within MIT1002_01378 at 1529.247 kb on + strand, within MIT1002_01378 at 1529.250 kb on - strand, within MIT1002_01378 at 1529.255 kb on - strand, within MIT1002_01378 at 1529.255 kb on - strand, within MIT1002_01378 at 1529.267 kb on + strand, within MIT1002_01378 at 1529.275 kb on - strand, within MIT1002_01378 at 1529.297 kb on + strand, within MIT1002_01378 at 1529.317 kb on - strand, within MIT1002_01378 at 1529.324 kb on + strand, within MIT1002_01378 at 1529.324 kb on + strand, within MIT1002_01378 at 1529.329 kb on + strand, within MIT1002_01378 at 1529.338 kb on + strand, within MIT1002_01378 at 1529.356 kb on - strand, within MIT1002_01378 at 1529.356 kb on - strand, within MIT1002_01378 at 1529.395 kb on + strand, within MIT1002_01378 at 1529.424 kb on + strand, within MIT1002_01378 at 1529.424 kb on + strand, within MIT1002_01378 at 1529.439 kb on + strand, within MIT1002_01378 at 1529.439 kb on + strand, within MIT1002_01378 at 1529.447 kb on - strand, within MIT1002_01378 at 1529.447 kb on - strand, within MIT1002_01378 at 1529.495 kb on + strand, within MIT1002_01378 at 1529.540 kb on + strand, within MIT1002_01378 at 1529.555 kb on - strand, within MIT1002_01378 at 1529.561 kb on - strand, within MIT1002_01378 at 1529.594 kb on - strand, within MIT1002_01378 at 1529.735 kb on + strand, within MIT1002_01378 at 1529.813 kb on - strand, within MIT1002_01378 at 1529.828 kb on - strand, within MIT1002_01378 at 1529.868 kb on - strand, within MIT1002_01378 at 1529.872 kb on + strand, within MIT1002_01378 at 1529.886 kb on + strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.894 kb on - strand, within MIT1002_01378 at 1529.945 kb on + strand, within MIT1002_01378 at 1530.003 kb on + strand, within MIT1002_01378 at 1530.008 kb on + strand, within MIT1002_01378 at 1530.013 kb on + strand, within MIT1002_01378 at 1530.016 kb on - strand, within MIT1002_01378 at 1530.017 kb on + strand, within MIT1002_01378 at 1530.084 kb on + strand, within MIT1002_01378 at 1530.084 kb on + strand, within MIT1002_01378 at 1530.144 kb on + strand at 1530.145 kb on + strand at 1530.145 kb on + strand at 1530.145 kb on + strand at 1530.145 kb on + strand at 1530.151 kb on - strand at 1530.151 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.153 kb on - strand at 1530.253 kb on + strand at 1530.253 kb on + strand at 1530.253 kb on + strand at 1530.261 kb on - strand at 1530.377 kb on + strand at 1530.525 kb on - strand, within MIT1002_01379 at 1530.660 kb on - strand, within MIT1002_01379 at 1530.695 kb on + strand, within MIT1002_01379 at 1530.695 kb on - strand, within MIT1002_01379 at 1530.710 kb on + strand, within MIT1002_01379 at 1530.710 kb on - strand, within MIT1002_01379 at 1530.727 kb on - strand, within MIT1002_01379 at 1530.782 kb on + strand, within MIT1002_01379 at 1530.782 kb on + strand, within MIT1002_01379 at 1530.830 kb on + strand, within MIT1002_01379 at 1530.833 kb on - strand, within MIT1002_01379 at 1530.838 kb on - strand, within MIT1002_01379 at 1530.880 kb on - strand, within MIT1002_01379 at 1530.909 kb on + strand, within MIT1002_01379 at 1530.932 kb on + strand, within MIT1002_01379 at 1530.932 kb on + strand, within MIT1002_01379 at 1530.957 kb on + strand, within MIT1002_01379 at 1530.957 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.967 kb on + strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1530.975 kb on - strand, within MIT1002_01379 at 1531.031 kb on - strand, within MIT1002_01379 at 1531.031 kb on - strand, within MIT1002_01379 at 1531.097 kb on - strand, within MIT1002_01379 at 1531.102 kb on - strand, within MIT1002_01379 at 1531.121 kb on + strand, within MIT1002_01379 at 1531.198 kb on - strand, within MIT1002_01379
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 1,527,936 - -0.6 1,527,946 - -0.3 1,527,985 - +1.1 1,528,066 - +0.5 1,528,099 - +0.6 1,528,154 - -0.1 1,528,154 - +0.6 1,528,167 - -0.2 1,528,217 + -1.6 1,528,262 - -0.0 1,528,356 + MIT1002_01377 0.24 +0.5 1,528,374 - MIT1002_01377 0.27 +1.2 1,528,374 - MIT1002_01377 0.27 +0.2 1,528,448 + MIT1002_01377 0.41 -0.1 1,528,488 - MIT1002_01377 0.49 -0.7 1,528,488 - MIT1002_01377 0.49 +0.2 1,528,505 + MIT1002_01377 0.52 +1.4 1,528,562 + MIT1002_01377 0.63 -2.1 1,528,582 - MIT1002_01377 0.67 -0.1 1,528,755 - -0.7 1,528,755 - +0.0 1,528,830 - +0.7 1,528,839 + +2.4 1,528,846 + +0.9 1,528,847 - -0.9 1,528,873 + -0.5 1,528,970 + -3.4 1,529,029 - -1.1 1,529,077 + MIT1002_01378 0.11 -2.5 1,529,114 + MIT1002_01378 0.14 +0.4 1,529,115 - MIT1002_01378 0.14 -2.0 1,529,120 + MIT1002_01378 0.15 -2.0 1,529,128 - MIT1002_01378 0.15 -1.5 1,529,247 + MIT1002_01378 0.24 -1.3 1,529,247 + MIT1002_01378 0.24 -2.3 1,529,247 + MIT1002_01378 0.24 -1.6 1,529,250 - MIT1002_01378 0.24 -0.6 1,529,255 - MIT1002_01378 0.25 -5.4 1,529,255 - MIT1002_01378 0.25 -5.0 1,529,267 + MIT1002_01378 0.26 -3.0 1,529,275 - MIT1002_01378 0.26 -1.3 1,529,297 + MIT1002_01378 0.28 -3.1 1,529,317 - MIT1002_01378 0.29 -3.0 1,529,324 + MIT1002_01378 0.30 -1.9 1,529,324 + MIT1002_01378 0.30 +0.4 1,529,329 + MIT1002_01378 0.30 -2.3 1,529,338 + MIT1002_01378 0.31 -2.5 1,529,356 - MIT1002_01378 0.32 -2.3 1,529,356 - MIT1002_01378 0.32 -1.3 1,529,395 + MIT1002_01378 0.35 -2.6 1,529,424 + MIT1002_01378 0.38 -2.6 1,529,424 + MIT1002_01378 0.38 -0.6 1,529,439 + MIT1002_01378 0.39 -2.7 1,529,439 + MIT1002_01378 0.39 -2.6 1,529,447 - MIT1002_01378 0.39 -2.1 1,529,447 - MIT1002_01378 0.39 -2.8 1,529,495 + MIT1002_01378 0.43 -2.0 1,529,540 + MIT1002_01378 0.46 -2.8 1,529,555 - MIT1002_01378 0.47 -2.1 1,529,561 - MIT1002_01378 0.48 -4.2 1,529,594 - MIT1002_01378 0.50 -2.5 1,529,735 + MIT1002_01378 0.61 -2.5 1,529,813 - MIT1002_01378 0.67 -2.3 1,529,828 - MIT1002_01378 0.68 +0.4 1,529,868 - MIT1002_01378 0.71 -2.2 1,529,872 + MIT1002_01378 0.71 -1.7 1,529,886 + MIT1002_01378 0.72 -3.2 1,529,894 - MIT1002_01378 0.73 -0.4 1,529,894 - MIT1002_01378 0.73 -2.3 1,529,894 - MIT1002_01378 0.73 -0.1 1,529,894 - MIT1002_01378 0.73 -3.4 1,529,945 + MIT1002_01378 0.77 -4.8 1,530,003 + MIT1002_01378 0.81 -5.7 1,530,008 + MIT1002_01378 0.82 -3.8 1,530,013 + MIT1002_01378 0.82 -0.1 1,530,016 - MIT1002_01378 0.82 -6.5 1,530,017 + MIT1002_01378 0.82 -2.8 1,530,084 + MIT1002_01378 0.87 -2.6 1,530,084 + MIT1002_01378 0.87 -1.6 1,530,144 + -4.3 1,530,145 + -6.3 1,530,145 + -0.4 1,530,145 + -4.4 1,530,145 + -3.6 1,530,151 - -2.8 1,530,151 - -2.2 1,530,153 - -2.9 1,530,153 - -1.6 1,530,153 - -3.9 1,530,153 - -0.7 1,530,153 - -1.5 1,530,253 + -2.0 1,530,253 + -2.5 1,530,253 + -5.6 1,530,261 - -1.9 1,530,377 + -3.9 1,530,525 - MIT1002_01379 0.21 -3.1 1,530,660 - MIT1002_01379 0.33 -0.9 1,530,695 + MIT1002_01379 0.36 -1.4 1,530,695 - MIT1002_01379 0.36 -3.3 1,530,710 + MIT1002_01379 0.37 -4.0 1,530,710 - MIT1002_01379 0.37 -3.3 1,530,727 - MIT1002_01379 0.39 -0.7 1,530,782 + MIT1002_01379 0.44 -3.6 1,530,782 + MIT1002_01379 0.44 -2.4 1,530,830 + MIT1002_01379 0.48 -3.5 1,530,833 - MIT1002_01379 0.48 -3.6 1,530,838 - MIT1002_01379 0.49 -3.8 1,530,880 - MIT1002_01379 0.52 -3.7 1,530,909 + MIT1002_01379 0.55 -2.1 1,530,932 + MIT1002_01379 0.57 -1.9 1,530,932 + MIT1002_01379 0.57 -3.4 1,530,957 + MIT1002_01379 0.59 -1.6 1,530,957 + MIT1002_01379 0.59 -1.4 1,530,967 + MIT1002_01379 0.60 -0.9 1,530,967 + MIT1002_01379 0.60 -6.9 1,530,967 + MIT1002_01379 0.60 -2.4 1,530,967 + MIT1002_01379 0.60 -2.6 1,530,967 + MIT1002_01379 0.60 -6.8 1,530,967 + MIT1002_01379 0.60 -3.2 1,530,975 - MIT1002_01379 0.61 -7.7 1,530,975 - MIT1002_01379 0.61 -1.1 1,530,975 - MIT1002_01379 0.61 -3.5 1,530,975 - MIT1002_01379 0.61 -2.5 1,530,975 - MIT1002_01379 0.61 -2.7 1,531,031 - MIT1002_01379 0.66 -3.1 1,531,031 - MIT1002_01379 0.66 -1.8 1,531,097 - MIT1002_01379 0.72 -2.7 1,531,102 - MIT1002_01379 0.72 -0.6 1,531,121 + MIT1002_01379 0.74 -2.3 1,531,198 - MIT1002_01379 0.81 -2.4
Or see this region's nucleotide sequence