Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01221 and MIT1002_01222 are separated by 74 nucleotides MIT1002_01222 and MIT1002_01223 are separated by 67 nucleotides MIT1002_01223 and MIT1002_01224 are separated by 282 nucleotides
MIT1002_01221: MIT1002_01221 - protease3, at 1,343,435 to 1,346,320
_01221
MIT1002_01222: MIT1002_01222 - Glutamate--cysteine ligase, at 1,346,395 to 1,347,993
_01222
MIT1002_01223: MIT1002_01223 - hypothetical protein, at 1,348,061 to 1,348,699
_01223
MIT1002_01224: MIT1002_01224 - hypothetical protein, at 1,348,982 to 1,349,440
_01224
Position (kb)
1346
1347
1348 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1345.460 kb on + strand, within MIT1002_01221 at 1345.498 kb on - strand, within MIT1002_01221 at 1345.628 kb on + strand, within MIT1002_01221 at 1345.635 kb on - strand, within MIT1002_01221 at 1345.701 kb on + strand, within MIT1002_01221 at 1345.704 kb on - strand, within MIT1002_01221 at 1345.709 kb on - strand, within MIT1002_01221 at 1345.771 kb on + strand, within MIT1002_01221 at 1345.835 kb on - strand, within MIT1002_01221 at 1345.844 kb on + strand, within MIT1002_01221 at 1345.858 kb on - strand, within MIT1002_01221 at 1345.940 kb on + strand, within MIT1002_01221 at 1345.940 kb on + strand, within MIT1002_01221 at 1345.940 kb on + strand, within MIT1002_01221 at 1345.940 kb on + strand, within MIT1002_01221 at 1345.948 kb on - strand, within MIT1002_01221 at 1345.948 kb on - strand, within MIT1002_01221 at 1345.948 kb on - strand, within MIT1002_01221 at 1345.984 kb on + strand, within MIT1002_01221 at 1345.990 kb on + strand, within MIT1002_01221 at 1346.124 kb on + strand at 1346.124 kb on - strand at 1346.251 kb on + strand at 1346.309 kb on + strand at 1346.309 kb on + strand at 1346.359 kb on + strand at 1346.371 kb on + strand at 1346.422 kb on - strand at 1346.548 kb on - strand at 1347.193 kb on - strand, within MIT1002_01222 at 1347.255 kb on - strand, within MIT1002_01222 at 1347.314 kb on - strand, within MIT1002_01222 at 1347.328 kb on - strand, within MIT1002_01222 at 1347.800 kb on + strand, within MIT1002_01222 at 1347.808 kb on - strand, within MIT1002_01222 at 1348.259 kb on + strand, within MIT1002_01223 at 1348.342 kb on - strand, within MIT1002_01223 at 1348.389 kb on + strand, within MIT1002_01223 at 1348.527 kb on + strand, within MIT1002_01223 at 1348.544 kb on + strand, within MIT1002_01223 at 1348.544 kb on + strand, within MIT1002_01223 at 1348.544 kb on + strand, within MIT1002_01223 at 1348.544 kb on + strand, within MIT1002_01223 at 1348.544 kb on + strand, within MIT1002_01223 at 1348.578 kb on - strand, within MIT1002_01223 at 1348.599 kb on - strand, within MIT1002_01223 at 1348.632 kb on + strand, within MIT1002_01223 at 1348.635 kb on - strand, within MIT1002_01223 at 1348.642 kb on + strand at 1348.642 kb on + strand at 1348.643 kb on + strand at 1348.643 kb on + strand at 1348.650 kb on - strand at 1348.650 kb on - strand at 1348.962 kb on - strand at 1348.987 kb on - strand at 1348.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 1,345,460 + MIT1002_01221 0.70 -1.2 1,345,498 - MIT1002_01221 0.71 -0.2 1,345,628 + MIT1002_01221 0.76 -1.1 1,345,635 - MIT1002_01221 0.76 -1.8 1,345,701 + MIT1002_01221 0.79 -1.2 1,345,704 - MIT1002_01221 0.79 +0.3 1,345,709 - MIT1002_01221 0.79 +1.5 1,345,771 + MIT1002_01221 0.81 +0.4 1,345,835 - MIT1002_01221 0.83 -0.1 1,345,844 + MIT1002_01221 0.83 -1.2 1,345,858 - MIT1002_01221 0.84 +0.4 1,345,940 + MIT1002_01221 0.87 -0.5 1,345,940 + MIT1002_01221 0.87 +1.2 1,345,940 + MIT1002_01221 0.87 -0.6 1,345,940 + MIT1002_01221 0.87 +0.4 1,345,948 - MIT1002_01221 0.87 -0.3 1,345,948 - MIT1002_01221 0.87 -0.1 1,345,948 - MIT1002_01221 0.87 -0.9 1,345,984 + MIT1002_01221 0.88 -0.9 1,345,990 + MIT1002_01221 0.89 -0.1 1,346,124 + -0.5 1,346,124 - -0.8 1,346,251 + +0.4 1,346,309 + -0.1 1,346,309 + -0.3 1,346,359 + -0.8 1,346,371 + +0.4 1,346,422 - +0.5 1,346,548 - -0.3 1,347,193 - MIT1002_01222 0.50 +0.5 1,347,255 - MIT1002_01222 0.54 +0.5 1,347,314 - MIT1002_01222 0.57 -0.3 1,347,328 - MIT1002_01222 0.58 -1.7 1,347,800 + MIT1002_01222 0.88 +0.2 1,347,808 - MIT1002_01222 0.88 -1.6 1,348,259 + MIT1002_01223 0.31 +0.9 1,348,342 - MIT1002_01223 0.44 -1.4 1,348,389 + MIT1002_01223 0.51 -0.6 1,348,527 + MIT1002_01223 0.73 -0.8 1,348,544 + MIT1002_01223 0.76 +1.2 1,348,544 + MIT1002_01223 0.76 -0.1 1,348,544 + MIT1002_01223 0.76 -1.6 1,348,544 + MIT1002_01223 0.76 +0.3 1,348,544 + MIT1002_01223 0.76 +0.1 1,348,578 - MIT1002_01223 0.81 -2.2 1,348,599 - MIT1002_01223 0.84 -0.5 1,348,632 + MIT1002_01223 0.89 +1.2 1,348,635 - MIT1002_01223 0.90 +0.4 1,348,642 + -1.6 1,348,642 + +1.0 1,348,643 + -1.8 1,348,643 + +0.1 1,348,650 - -0.8 1,348,650 - -2.5 1,348,962 - -0.4 1,348,987 - -1.1 1,348,993 - -3.0
Or see this region's nucleotide sequence