Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01020 and MIT1002_01021 are separated by 125 nucleotides MIT1002_01021 and MIT1002_01022 are separated by 263 nucleotides
MIT1002_01020: MIT1002_01020 - tRNA(Ile)-lysidine synthase, at 1,130,030 to 1,131,355
_01020
MIT1002_01021: MIT1002_01021 - putative diguanylate cyclase YedQ, at 1,131,481 to 1,132,380
_01021
MIT1002_01022: MIT1002_01022 - NEM-activable K(+)/H(+) antiporter, at 1,132,644 to 1,134,611
_01022
Position (kb)
1131
1132
1133 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1131.340 kb on + strand at 1131.478 kb on + strand at 1131.486 kb on - strand at 1131.486 kb on - strand at 1131.523 kb on + strand at 1131.531 kb on - strand at 1131.544 kb on + strand at 1131.643 kb on + strand, within MIT1002_01021 at 1131.660 kb on - strand, within MIT1002_01021 at 1131.662 kb on + strand, within MIT1002_01021 at 1131.662 kb on + strand, within MIT1002_01021 at 1131.665 kb on - strand, within MIT1002_01021 at 1131.670 kb on - strand, within MIT1002_01021 at 1131.692 kb on - strand, within MIT1002_01021 at 1131.709 kb on - strand, within MIT1002_01021 at 1131.855 kb on - strand, within MIT1002_01021 at 1131.928 kb on + strand, within MIT1002_01021 at 1131.928 kb on + strand, within MIT1002_01021 at 1131.971 kb on - strand, within MIT1002_01021 at 1132.139 kb on - strand, within MIT1002_01021 at 1132.139 kb on - strand, within MIT1002_01021 at 1132.153 kb on + strand, within MIT1002_01021 at 1132.153 kb on + strand, within MIT1002_01021 at 1132.161 kb on - strand, within MIT1002_01021 at 1132.161 kb on - strand, within MIT1002_01021 at 1132.192 kb on + strand, within MIT1002_01021 at 1132.318 kb on + strand at 1132.318 kb on + strand at 1132.326 kb on - strand at 1132.352 kb on - strand at 1132.354 kb on + strand at 1132.354 kb on + strand at 1132.390 kb on - strand at 1132.432 kb on + strand at 1132.818 kb on + strand at 1132.916 kb on - strand, within MIT1002_01022 at 1132.938 kb on + strand, within MIT1002_01022 at 1132.946 kb on - strand, within MIT1002_01022 at 1132.948 kb on - strand, within MIT1002_01022 at 1132.956 kb on - strand, within MIT1002_01022 at 1133.025 kb on + strand, within MIT1002_01022 at 1133.041 kb on + strand, within MIT1002_01022 at 1133.068 kb on + strand, within MIT1002_01022 at 1133.115 kb on - strand, within MIT1002_01022 at 1133.147 kb on + strand, within MIT1002_01022 at 1133.170 kb on - strand, within MIT1002_01022 at 1133.177 kb on + strand, within MIT1002_01022 at 1133.185 kb on - strand, within MIT1002_01022 at 1133.195 kb on + strand, within MIT1002_01022 at 1133.195 kb on + strand, within MIT1002_01022 at 1133.203 kb on - strand, within MIT1002_01022 at 1133.203 kb on - strand, within MIT1002_01022 at 1133.203 kb on - strand, within MIT1002_01022 at 1133.243 kb on - strand, within MIT1002_01022 at 1133.243 kb on - strand, within MIT1002_01022 at 1133.250 kb on + strand, within MIT1002_01022 at 1133.284 kb on - strand, within MIT1002_01022 at 1133.298 kb on + strand, within MIT1002_01022 at 1133.324 kb on + strand, within MIT1002_01022
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 1,131,340 + -0.9 1,131,478 + +0.3 1,131,486 - +0.5 1,131,486 - +1.4 1,131,523 + +0.6 1,131,531 - -1.4 1,131,544 + +0.3 1,131,643 + MIT1002_01021 0.18 -2.0 1,131,660 - MIT1002_01021 0.20 +0.8 1,131,662 + MIT1002_01021 0.20 -0.5 1,131,662 + MIT1002_01021 0.20 -1.0 1,131,665 - MIT1002_01021 0.20 +1.0 1,131,670 - MIT1002_01021 0.21 +0.6 1,131,692 - MIT1002_01021 0.23 +0.0 1,131,709 - MIT1002_01021 0.25 +0.1 1,131,855 - MIT1002_01021 0.42 -0.9 1,131,928 + MIT1002_01021 0.50 -0.5 1,131,928 + MIT1002_01021 0.50 -1.2 1,131,971 - MIT1002_01021 0.54 -2.9 1,132,139 - MIT1002_01021 0.73 +0.4 1,132,139 - MIT1002_01021 0.73 +0.5 1,132,153 + MIT1002_01021 0.75 -1.1 1,132,153 + MIT1002_01021 0.75 -0.1 1,132,161 - MIT1002_01021 0.76 -0.2 1,132,161 - MIT1002_01021 0.76 -0.6 1,132,192 + MIT1002_01021 0.79 -2.4 1,132,318 + +0.7 1,132,318 + -0.0 1,132,326 - -0.4 1,132,352 - -0.6 1,132,354 + -0.8 1,132,354 + -0.0 1,132,390 - +0.4 1,132,432 + +0.8 1,132,818 + +0.3 1,132,916 - MIT1002_01022 0.14 -0.1 1,132,938 + MIT1002_01022 0.15 -0.6 1,132,946 - MIT1002_01022 0.15 -3.2 1,132,948 - MIT1002_01022 0.15 +1.6 1,132,956 - MIT1002_01022 0.16 +1.5 1,133,025 + MIT1002_01022 0.19 +0.2 1,133,041 + MIT1002_01022 0.20 +0.6 1,133,068 + MIT1002_01022 0.22 +1.4 1,133,115 - MIT1002_01022 0.24 -0.1 1,133,147 + MIT1002_01022 0.26 -1.5 1,133,170 - MIT1002_01022 0.27 +1.6 1,133,177 + MIT1002_01022 0.27 +1.0 1,133,185 - MIT1002_01022 0.27 -0.8 1,133,195 + MIT1002_01022 0.28 -2.7 1,133,195 + MIT1002_01022 0.28 -1.0 1,133,203 - MIT1002_01022 0.28 -0.9 1,133,203 - MIT1002_01022 0.28 +1.0 1,133,203 - MIT1002_01022 0.28 -1.2 1,133,243 - MIT1002_01022 0.30 +1.6 1,133,243 - MIT1002_01022 0.30 +0.5 1,133,250 + MIT1002_01022 0.31 -1.2 1,133,284 - MIT1002_01022 0.33 -0.0 1,133,298 + MIT1002_01022 0.33 +0.2 1,133,324 + MIT1002_01022 0.35 -1.4
Or see this region's nucleotide sequence