Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00758 and MIT1002_00759 are separated by 2 nucleotides MIT1002_00759 and MIT1002_00760 are separated by 32 nucleotides MIT1002_00760 and MIT1002_00761 are separated by 3 nucleotides MIT1002_00761 and MIT1002_00762 are separated by 6 nucleotides
MIT1002_00758: MIT1002_00758 - Biopolymer transport protein ExbB, at 834,196 to 835,560
_00758
MIT1002_00759: MIT1002_00759 - protein TolQ, at 835,563 to 836,081
_00759
MIT1002_00760: MIT1002_00760 - Biopolymer transport protein ExbD, at 836,114 to 836,521
_00760
MIT1002_00761: MIT1002_00761 - transport protein TonB, at 836,525 to 837,133
_00761
MIT1002_00762: MIT1002_00762 - Anaphase-promoting complex, cyclosome, subunit 3, at 837,140 to 838,498
_00762
Position (kb)
836
837 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 835.117 kb on - strand, within MIT1002_00758 at 835.134 kb on + strand, within MIT1002_00758 at 835.316 kb on - strand, within MIT1002_00758 at 835.341 kb on + strand, within MIT1002_00758 at 835.341 kb on + strand, within MIT1002_00758 at 835.349 kb on - strand, within MIT1002_00758 at 835.349 kb on - strand, within MIT1002_00758 at 835.376 kb on - strand, within MIT1002_00758 at 835.376 kb on - strand, within MIT1002_00758 at 835.437 kb on - strand at 835.464 kb on + strand at 835.472 kb on - strand at 835.472 kb on - strand at 835.472 kb on - strand at 835.472 kb on - strand at 835.476 kb on + strand at 835.477 kb on - strand at 835.477 kb on - strand at 835.485 kb on - strand at 835.509 kb on - strand at 835.555 kb on + strand at 835.562 kb on - strand at 835.614 kb on + strand at 835.630 kb on - strand, within MIT1002_00759 at 835.659 kb on + strand, within MIT1002_00759 at 835.734 kb on - strand, within MIT1002_00759 at 835.865 kb on - strand, within MIT1002_00759 at 835.922 kb on - strand, within MIT1002_00759 at 835.928 kb on - strand, within MIT1002_00759 at 835.937 kb on + strand, within MIT1002_00759 at 836.057 kb on - strand at 836.057 kb on - strand at 836.084 kb on - strand at 836.092 kb on + strand at 836.100 kb on - strand at 836.249 kb on - strand, within MIT1002_00760 at 836.256 kb on + strand, within MIT1002_00760 at 836.363 kb on + strand, within MIT1002_00760 at 836.382 kb on - strand, within MIT1002_00760 at 836.404 kb on + strand, within MIT1002_00760 at 836.515 kb on - strand at 836.576 kb on - strand at 836.632 kb on + strand, within MIT1002_00761 at 836.661 kb on + strand, within MIT1002_00761 at 836.669 kb on - strand, within MIT1002_00761 at 836.726 kb on - strand, within MIT1002_00761 at 836.749 kb on + strand, within MIT1002_00761 at 836.869 kb on - strand, within MIT1002_00761 at 836.930 kb on - strand, within MIT1002_00761 at 836.930 kb on - strand, within MIT1002_00761 at 837.113 kb on - strand at 837.174 kb on - strand at 837.195 kb on + strand at 837.201 kb on + strand at 837.249 kb on + strand at 837.270 kb on - strand at 837.274 kb on - strand at 837.306 kb on + strand, within MIT1002_00762 at 837.343 kb on - strand, within MIT1002_00762 at 837.352 kb on + strand, within MIT1002_00762 at 837.352 kb on + strand, within MIT1002_00762 at 837.418 kb on + strand, within MIT1002_00762 at 837.485 kb on + strand, within MIT1002_00762 at 837.485 kb on + strand, within MIT1002_00762 at 837.503 kb on + strand, within MIT1002_00762 at 837.503 kb on + strand, within MIT1002_00762 at 837.517 kb on + strand, within MIT1002_00762
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 835,117 - MIT1002_00758 0.67 -4.0 835,134 + MIT1002_00758 0.69 -3.0 835,316 - MIT1002_00758 0.82 -0.7 835,341 + MIT1002_00758 0.84 -3.5 835,341 + MIT1002_00758 0.84 -4.4 835,349 - MIT1002_00758 0.84 -2.5 835,349 - MIT1002_00758 0.84 -2.0 835,376 - MIT1002_00758 0.86 -2.8 835,376 - MIT1002_00758 0.86 -2.8 835,437 - -4.4 835,464 + -2.0 835,472 - -3.4 835,472 - -0.5 835,472 - -2.2 835,472 - +0.6 835,476 + -3.7 835,477 - -4.4 835,477 - -1.6 835,485 - -2.7 835,509 - -2.7 835,555 + -2.5 835,562 - -3.5 835,614 + -6.5 835,630 - MIT1002_00759 0.13 -3.4 835,659 + MIT1002_00759 0.18 -1.8 835,734 - MIT1002_00759 0.33 -2.8 835,865 - MIT1002_00759 0.58 -4.1 835,922 - MIT1002_00759 0.69 -2.3 835,928 - MIT1002_00759 0.70 -1.8 835,937 + MIT1002_00759 0.72 -2.1 836,057 - -3.0 836,057 - -2.2 836,084 - -1.8 836,092 + -0.5 836,100 - -3.2 836,249 - MIT1002_00760 0.33 -3.1 836,256 + MIT1002_00760 0.35 -2.6 836,363 + MIT1002_00760 0.61 -2.1 836,382 - MIT1002_00760 0.66 -3.0 836,404 + MIT1002_00760 0.71 +0.2 836,515 - -2.8 836,576 - -4.1 836,632 + MIT1002_00761 0.18 -2.9 836,661 + MIT1002_00761 0.22 -3.3 836,669 - MIT1002_00761 0.24 -2.7 836,726 - MIT1002_00761 0.33 -1.2 836,749 + MIT1002_00761 0.37 -0.7 836,869 - MIT1002_00761 0.56 -3.3 836,930 - MIT1002_00761 0.67 -1.4 836,930 - MIT1002_00761 0.67 -3.6 837,113 - -2.3 837,174 - -1.7 837,195 + -1.7 837,201 + -2.7 837,249 + -2.5 837,270 - -1.0 837,274 - -1.8 837,306 + MIT1002_00762 0.12 -3.7 837,343 - MIT1002_00762 0.15 -4.1 837,352 + MIT1002_00762 0.16 -3.0 837,352 + MIT1002_00762 0.16 -1.8 837,418 + MIT1002_00762 0.20 -1.8 837,485 + MIT1002_00762 0.25 -1.2 837,485 + MIT1002_00762 0.25 -2.0 837,503 + MIT1002_00762 0.27 -0.4 837,503 + MIT1002_00762 0.27 -1.0 837,517 + MIT1002_00762 0.28 -1.1
Or see this region's nucleotide sequence