Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00740 and MIT1002_00741 are separated by 281 nucleotides MIT1002_00741 and MIT1002_00742 overlap by 1 nucleotides MIT1002_00742 and MIT1002_00743 are separated by 8 nucleotides MIT1002_00743 and MIT1002_00744 are separated by 176 nucleotides
MIT1002_00740: MIT1002_00740 - G3P acyltransferase, at 812,687 to 813,289
_00740
MIT1002_00741: MIT1002_00741 - Dihydroneopterin aldolase, at 813,571 to 813,924
_00741
MIT1002_00742: MIT1002_00742 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 813,924 to 814,418
_00742
MIT1002_00743: MIT1002_00743 - Undecaprenyl-diphosphatase, at 814,427 to 815,227
_00743
MIT1002_00744: MIT1002_00744 - HIT-like protein, at 815,404 to 815,775
_00744
Position (kb)
813
814
815 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 812.945 kb on - strand, within MIT1002_00740 at 812.952 kb on - strand, within MIT1002_00740 at 813.154 kb on + strand, within MIT1002_00740 at 813.209 kb on - strand, within MIT1002_00740 at 813.409 kb on + strand at 813.409 kb on + strand at 813.457 kb on + strand at 813.499 kb on + strand at 813.509 kb on + strand at 813.509 kb on + strand at 813.938 kb on - strand at 813.964 kb on - strand at 813.982 kb on - strand, within MIT1002_00742 at 814.013 kb on + strand, within MIT1002_00742 at 814.023 kb on - strand, within MIT1002_00742 at 814.031 kb on + strand, within MIT1002_00742 at 814.061 kb on - strand, within MIT1002_00742 at 814.122 kb on - strand, within MIT1002_00742 at 814.218 kb on + strand, within MIT1002_00742 at 814.237 kb on + strand, within MIT1002_00742 at 814.253 kb on - strand, within MIT1002_00742 at 814.282 kb on + strand, within MIT1002_00742 at 814.282 kb on + strand, within MIT1002_00742 at 814.396 kb on - strand at 815.209 kb on - strand at 815.273 kb on + strand at 815.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 812,945 - MIT1002_00740 0.43 +0.1 812,952 - MIT1002_00740 0.44 +1.2 813,154 + MIT1002_00740 0.77 +0.2 813,209 - MIT1002_00740 0.87 -0.8 813,409 + +0.1 813,409 + -1.5 813,457 + -1.3 813,499 + -1.5 813,509 + +1.6 813,509 + +0.5 813,938 - -2.8 813,964 - -1.2 813,982 - MIT1002_00742 0.12 -3.0 814,013 + MIT1002_00742 0.18 +0.3 814,023 - MIT1002_00742 0.20 -1.3 814,031 + MIT1002_00742 0.22 -3.8 814,061 - MIT1002_00742 0.28 -4.6 814,122 - MIT1002_00742 0.40 -3.4 814,218 + MIT1002_00742 0.59 -2.1 814,237 + MIT1002_00742 0.63 -0.5 814,253 - MIT1002_00742 0.66 -2.1 814,282 + MIT1002_00742 0.72 -3.4 814,282 + MIT1002_00742 0.72 -1.3 814,396 - -3.1 815,209 - +0.4 815,273 + +0.2 815,314 - -0.5
Or see this region's nucleotide sequence