Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00520 and MIT1002_00521 are separated by 278 nucleotides MIT1002_00521 and MIT1002_00522 overlap by 4 nucleotides MIT1002_00522 and MIT1002_00523 are separated by 60 nucleotides MIT1002_00523 and MIT1002_00524 are separated by 66 nucleotides MIT1002_00524 and MIT1002_00525 are separated by 186 nucleotides
MIT1002_00520: MIT1002_00520 - Sodium/proton antiporter NhaA, at 566,728 to 568,002
_00520
MIT1002_00521: MIT1002_00521 - N5-carboxyaminoimidazole ribonucleotide mutase, at 568,281 to 568,775
_00521
MIT1002_00522: MIT1002_00522 - N5-carboxyaminoimidazole ribonucleotide synthase, at 568,772 to 569,908
_00522
MIT1002_00523: MIT1002_00523 - tRNA-Thr, at 569,969 to 570,044
_00523
MIT1002_00524: MIT1002_00524 - hypothetical protein, at 570,111 to 570,656
_00524
MIT1002_00525: MIT1002_00525 - DNA protection during starvation protein 2, at 570,843 to 571,316
_00525
Position (kb) 568
569
570 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 567.837 kb on - strand, within MIT1002_00520 at 567.837 kb on - strand, within MIT1002_00520 at 567.954 kb on - strand at 567.954 kb on - strand at 568.030 kb on - strand at 568.150 kb on + strand at 568.158 kb on - strand at 568.165 kb on + strand at 568.195 kb on - strand at 568.319 kb on - strand at 568.337 kb on - strand, within MIT1002_00521 at 568.405 kb on - strand, within MIT1002_00521 at 568.405 kb on - strand, within MIT1002_00521 at 568.435 kb on + strand, within MIT1002_00521 at 568.440 kb on - strand, within MIT1002_00521 at 568.515 kb on + strand, within MIT1002_00521 at 568.551 kb on - strand, within MIT1002_00521 at 568.561 kb on + strand, within MIT1002_00521 at 568.574 kb on + strand, within MIT1002_00521 at 568.799 kb on - strand at 568.926 kb on + strand, within MIT1002_00522 at 568.943 kb on - strand, within MIT1002_00522 at 568.946 kb on - strand, within MIT1002_00522 at 568.978 kb on - strand, within MIT1002_00522 at 569.020 kb on - strand, within MIT1002_00522 at 569.157 kb on + strand, within MIT1002_00522 at 569.332 kb on + strand, within MIT1002_00522 at 569.364 kb on - strand, within MIT1002_00522 at 569.366 kb on - strand, within MIT1002_00522 at 569.366 kb on - strand, within MIT1002_00522 at 569.398 kb on - strand, within MIT1002_00522 at 569.449 kb on + strand, within MIT1002_00522 at 569.479 kb on + strand, within MIT1002_00522 at 569.480 kb on + strand, within MIT1002_00522 at 569.488 kb on - strand, within MIT1002_00522 at 569.515 kb on + strand, within MIT1002_00522 at 569.523 kb on - strand, within MIT1002_00522 at 569.576 kb on - strand, within MIT1002_00522 at 569.724 kb on + strand, within MIT1002_00522 at 569.732 kb on - strand, within MIT1002_00522 at 569.738 kb on + strand, within MIT1002_00522 at 569.738 kb on + strand, within MIT1002_00522 at 569.858 kb on + strand at 569.866 kb on - strand at 570.089 kb on - strand at 570.178 kb on - strand, within MIT1002_00524 at 570.279 kb on + strand, within MIT1002_00524 at 570.279 kb on + strand, within MIT1002_00524 at 570.293 kb on + strand, within MIT1002_00524 at 570.294 kb on + strand, within MIT1002_00524 at 570.323 kb on + strand, within MIT1002_00524 at 570.333 kb on - strand, within MIT1002_00524 at 570.338 kb on + strand, within MIT1002_00524 at 570.363 kb on - strand, within MIT1002_00524 at 570.493 kb on + strand, within MIT1002_00524 at 570.536 kb on - strand, within MIT1002_00524 at 570.549 kb on - strand, within MIT1002_00524 at 570.564 kb on + strand, within MIT1002_00524 at 570.589 kb on + strand, within MIT1002_00524 at 570.604 kb on - strand at 570.719 kb on + strand at 570.826 kb on + strand at 570.828 kb on + strand at 570.834 kb on + strand at 570.842 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 567,837 - MIT1002_00520 0.87 +0.8 567,837 - MIT1002_00520 0.87 +0.0 567,954 - +0.4 567,954 - -0.7 568,030 - +0.1 568,150 + +0.8 568,158 - +1.6 568,165 + -1.4 568,195 - -1.0 568,319 - -0.2 568,337 - MIT1002_00521 0.11 -0.4 568,405 - MIT1002_00521 0.25 -2.5 568,405 - MIT1002_00521 0.25 -1.4 568,435 + MIT1002_00521 0.31 +0.1 568,440 - MIT1002_00521 0.32 -2.0 568,515 + MIT1002_00521 0.47 -0.2 568,551 - MIT1002_00521 0.55 -4.6 568,561 + MIT1002_00521 0.57 -2.5 568,574 + MIT1002_00521 0.59 -1.3 568,799 - -1.3 568,926 + MIT1002_00522 0.14 -2.9 568,943 - MIT1002_00522 0.15 -0.9 568,946 - MIT1002_00522 0.15 -4.8 568,978 - MIT1002_00522 0.18 -3.0 569,020 - MIT1002_00522 0.22 -4.2 569,157 + MIT1002_00522 0.34 -3.2 569,332 + MIT1002_00522 0.49 -4.6 569,364 - MIT1002_00522 0.52 +0.3 569,366 - MIT1002_00522 0.52 -0.7 569,366 - MIT1002_00522 0.52 -2.2 569,398 - MIT1002_00522 0.55 -3.6 569,449 + MIT1002_00522 0.60 -3.8 569,479 + MIT1002_00522 0.62 -1.9 569,480 + MIT1002_00522 0.62 -1.4 569,488 - MIT1002_00522 0.63 -2.9 569,515 + MIT1002_00522 0.65 -5.6 569,523 - MIT1002_00522 0.66 -3.2 569,576 - MIT1002_00522 0.71 -2.7 569,724 + MIT1002_00522 0.84 -0.8 569,732 - MIT1002_00522 0.84 +0.1 569,738 + MIT1002_00522 0.85 -4.4 569,738 + MIT1002_00522 0.85 -5.8 569,858 + -6.3 569,866 - -1.3 570,089 - -0.0 570,178 - MIT1002_00524 0.12 -0.2 570,279 + MIT1002_00524 0.31 -1.4 570,279 + MIT1002_00524 0.31 -0.8 570,293 + MIT1002_00524 0.33 +0.4 570,294 + MIT1002_00524 0.34 -0.4 570,323 + MIT1002_00524 0.39 +2.2 570,333 - MIT1002_00524 0.41 -1.4 570,338 + MIT1002_00524 0.42 -0.0 570,363 - MIT1002_00524 0.46 +0.9 570,493 + MIT1002_00524 0.70 -0.2 570,536 - MIT1002_00524 0.78 -0.9 570,549 - MIT1002_00524 0.80 -1.2 570,564 + MIT1002_00524 0.83 +0.9 570,589 + MIT1002_00524 0.88 +1.1 570,604 - -2.1 570,719 + +0.7 570,826 + -0.6 570,828 + +0.8 570,834 + -1.2 570,842 - +0.1
Or see this region's nucleotide sequence