Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00467 and MIT1002_00468 are separated by 53 nucleotides MIT1002_00468 and MIT1002_00469 are separated by 37 nucleotides MIT1002_00469 and MIT1002_00470 overlap by 4 nucleotides
MIT1002_00467: MIT1002_00467 - Argininosuccinate lyase, at 510,499 to 511,881
_00467
MIT1002_00468: MIT1002_00468 - Argininosuccinate synthase, at 511,935 to 513,146
_00468
MIT1002_00469: MIT1002_00469 - Ornithine carbamoyltransferase, at 513,184 to 514,095
_00469
MIT1002_00470: MIT1002_00470 - Acetylglutamate kinase, at 514,092 to 514,937
_00470
Position (kb)
511
512
513
514 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 510.946 kb on + strand, within MIT1002_00467 at 510.995 kb on + strand, within MIT1002_00467 at 511.052 kb on + strand, within MIT1002_00467 at 511.084 kb on + strand, within MIT1002_00467 at 511.092 kb on - strand, within MIT1002_00467 at 511.154 kb on - strand, within MIT1002_00467 at 511.183 kb on + strand, within MIT1002_00467 at 511.189 kb on + strand, within MIT1002_00467 at 511.189 kb on + strand, within MIT1002_00467 at 511.191 kb on - strand, within MIT1002_00467 at 511.197 kb on - strand, within MIT1002_00467 at 511.197 kb on - strand, within MIT1002_00467 at 511.200 kb on + strand, within MIT1002_00467 at 511.216 kb on - strand, within MIT1002_00467 at 511.218 kb on - strand, within MIT1002_00467 at 511.236 kb on + strand, within MIT1002_00467 at 511.293 kb on + strand, within MIT1002_00467 at 511.294 kb on + strand, within MIT1002_00467 at 511.294 kb on + strand, within MIT1002_00467 at 511.294 kb on + strand, within MIT1002_00467 at 511.302 kb on - strand, within MIT1002_00467 at 511.311 kb on - strand, within MIT1002_00467 at 511.338 kb on + strand, within MIT1002_00467 at 511.347 kb on - strand, within MIT1002_00467 at 511.372 kb on - strand, within MIT1002_00467 at 511.378 kb on + strand, within MIT1002_00467 at 511.378 kb on - strand, within MIT1002_00467 at 511.383 kb on - strand, within MIT1002_00467 at 511.398 kb on + strand, within MIT1002_00467 at 511.408 kb on + strand, within MIT1002_00467 at 511.408 kb on + strand, within MIT1002_00467 at 511.416 kb on - strand, within MIT1002_00467 at 511.424 kb on + strand, within MIT1002_00467 at 511.425 kb on - strand, within MIT1002_00467 at 511.498 kb on + strand, within MIT1002_00467 at 511.512 kb on - strand, within MIT1002_00467 at 511.566 kb on - strand, within MIT1002_00467 at 511.583 kb on - strand, within MIT1002_00467 at 511.693 kb on + strand, within MIT1002_00467 at 511.698 kb on + strand, within MIT1002_00467 at 511.698 kb on + strand, within MIT1002_00467 at 511.737 kb on - strand, within MIT1002_00467 at 511.782 kb on - strand at 511.828 kb on - strand at 511.870 kb on + strand at 511.878 kb on - strand at 511.880 kb on + strand at 511.890 kb on - strand at 511.901 kb on + strand at 511.906 kb on + strand at 511.906 kb on + strand at 511.909 kb on - strand at 511.909 kb on - strand at 511.914 kb on - strand at 511.921 kb on + strand at 511.921 kb on + strand at 511.940 kb on - strand at 511.986 kb on - strand at 512.000 kb on - strand at 512.084 kb on + strand, within MIT1002_00468 at 512.104 kb on + strand, within MIT1002_00468 at 512.128 kb on - strand, within MIT1002_00468 at 512.160 kb on + strand, within MIT1002_00468 at 512.207 kb on - strand, within MIT1002_00468 at 512.207 kb on - strand, within MIT1002_00468 at 512.289 kb on - strand, within MIT1002_00468 at 512.355 kb on + strand, within MIT1002_00468 at 512.375 kb on + strand, within MIT1002_00468 at 512.375 kb on + strand, within MIT1002_00468 at 512.383 kb on - strand, within MIT1002_00468 at 512.383 kb on - strand, within MIT1002_00468 at 512.383 kb on - strand, within MIT1002_00468 at 512.383 kb on - strand, within MIT1002_00468 at 512.383 kb on - strand, within MIT1002_00468 at 512.489 kb on + strand, within MIT1002_00468 at 512.490 kb on - strand, within MIT1002_00468 at 512.494 kb on + strand, within MIT1002_00468 at 512.497 kb on - strand, within MIT1002_00468 at 512.499 kb on + strand, within MIT1002_00468 at 512.502 kb on - strand, within MIT1002_00468 at 512.529 kb on + strand, within MIT1002_00468 at 512.532 kb on - strand, within MIT1002_00468 at 512.537 kb on - strand, within MIT1002_00468 at 512.599 kb on - strand, within MIT1002_00468 at 512.710 kb on + strand, within MIT1002_00468 at 512.726 kb on + strand, within MIT1002_00468 at 512.726 kb on + strand, within MIT1002_00468 at 512.777 kb on - strand, within MIT1002_00468 at 512.835 kb on - strand, within MIT1002_00468 at 512.866 kb on + strand, within MIT1002_00468 at 512.879 kb on + strand, within MIT1002_00468 at 512.888 kb on - strand, within MIT1002_00468 at 512.888 kb on - strand, within MIT1002_00468 at 512.912 kb on - strand, within MIT1002_00468 at 512.922 kb on + strand, within MIT1002_00468 at 512.930 kb on - strand, within MIT1002_00468 at 513.016 kb on + strand, within MIT1002_00468 at 513.029 kb on - strand at 513.107 kb on + strand at 513.112 kb on + strand at 513.112 kb on + strand at 513.120 kb on - strand at 513.232 kb on + strand at 513.308 kb on + strand, within MIT1002_00469 at 513.326 kb on - strand, within MIT1002_00469 at 513.356 kb on + strand, within MIT1002_00469 at 513.356 kb on + strand, within MIT1002_00469 at 513.393 kb on + strand, within MIT1002_00469 at 513.401 kb on - strand, within MIT1002_00469 at 513.439 kb on + strand, within MIT1002_00469 at 513.439 kb on + strand, within MIT1002_00469 at 513.447 kb on - strand, within MIT1002_00469 at 513.447 kb on - strand, within MIT1002_00469 at 513.461 kb on + strand, within MIT1002_00469 at 513.461 kb on + strand, within MIT1002_00469 at 513.461 kb on + strand, within MIT1002_00469 at 513.469 kb on - strand, within MIT1002_00469 at 513.469 kb on - strand, within MIT1002_00469 at 513.552 kb on + strand, within MIT1002_00469 at 513.552 kb on + strand, within MIT1002_00469 at 513.552 kb on + strand, within MIT1002_00469 at 513.552 kb on + strand, within MIT1002_00469 at 513.560 kb on - strand, within MIT1002_00469 at 513.580 kb on + strand, within MIT1002_00469 at 513.620 kb on - strand, within MIT1002_00469 at 513.635 kb on + strand, within MIT1002_00469 at 513.646 kb on + strand, within MIT1002_00469 at 513.653 kb on - strand, within MIT1002_00469 at 513.654 kb on - strand, within MIT1002_00469 at 513.700 kb on + strand, within MIT1002_00469 at 513.806 kb on + strand, within MIT1002_00469 at 513.833 kb on - strand, within MIT1002_00469 at 513.881 kb on + strand, within MIT1002_00469 at 513.887 kb on - strand, within MIT1002_00469 at 513.887 kb on - strand, within MIT1002_00469 at 513.887 kb on - strand, within MIT1002_00469 at 513.928 kb on + strand, within MIT1002_00469 at 513.963 kb on + strand, within MIT1002_00469 at 514.068 kb on + strand at 514.075 kb on + strand at 514.075 kb on + strand at 514.083 kb on - strand at 514.083 kb on - strand at 514.083 kb on - strand at 514.083 kb on - strand at 514.083 kb on - strand at 514.083 kb on - strand at 514.083 kb on - strand at 514.089 kb on + strand at 514.134 kb on + strand at 514.134 kb on + strand at 514.142 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 510,946 + MIT1002_00467 0.32 -5.5 510,995 + MIT1002_00467 0.36 -3.8 511,052 + MIT1002_00467 0.40 -3.9 511,084 + MIT1002_00467 0.42 -2.5 511,092 - MIT1002_00467 0.43 +0.1 511,154 - MIT1002_00467 0.47 -1.9 511,183 + MIT1002_00467 0.49 -1.9 511,189 + MIT1002_00467 0.50 -3.2 511,189 + MIT1002_00467 0.50 -1.7 511,191 - MIT1002_00467 0.50 -3.5 511,197 - MIT1002_00467 0.50 -0.7 511,197 - MIT1002_00467 0.50 -1.8 511,200 + MIT1002_00467 0.51 -3.2 511,216 - MIT1002_00467 0.52 -0.8 511,218 - MIT1002_00467 0.52 -3.3 511,236 + MIT1002_00467 0.53 -1.5 511,293 + MIT1002_00467 0.57 -1.9 511,294 + MIT1002_00467 0.57 -3.8 511,294 + MIT1002_00467 0.57 -1.5 511,294 + MIT1002_00467 0.57 -1.3 511,302 - MIT1002_00467 0.58 -4.8 511,311 - MIT1002_00467 0.59 -0.5 511,338 + MIT1002_00467 0.61 -2.5 511,347 - MIT1002_00467 0.61 -3.6 511,372 - MIT1002_00467 0.63 -3.4 511,378 + MIT1002_00467 0.64 -3.3 511,378 - MIT1002_00467 0.64 -1.6 511,383 - MIT1002_00467 0.64 +0.1 511,398 + MIT1002_00467 0.65 -3.4 511,408 + MIT1002_00467 0.66 -2.2 511,408 + MIT1002_00467 0.66 -1.5 511,416 - MIT1002_00467 0.66 -1.9 511,424 + MIT1002_00467 0.67 -3.2 511,425 - MIT1002_00467 0.67 -4.4 511,498 + MIT1002_00467 0.72 -4.1 511,512 - MIT1002_00467 0.73 -3.3 511,566 - MIT1002_00467 0.77 -6.8 511,583 - MIT1002_00467 0.78 -3.2 511,693 + MIT1002_00467 0.86 -4.0 511,698 + MIT1002_00467 0.87 -2.9 511,698 + MIT1002_00467 0.87 -2.7 511,737 - MIT1002_00467 0.90 -6.2 511,782 - -1.6 511,828 - -4.9 511,870 + -4.1 511,878 - -2.9 511,880 + -1.5 511,890 - -0.6 511,901 + -2.9 511,906 + -1.2 511,906 + -1.2 511,909 - -0.7 511,909 - +0.5 511,914 - -0.2 511,921 + -1.8 511,921 + -4.0 511,940 - -1.1 511,986 - -3.2 512,000 - -2.4 512,084 + MIT1002_00468 0.12 -3.0 512,104 + MIT1002_00468 0.14 -3.0 512,128 - MIT1002_00468 0.16 -2.8 512,160 + MIT1002_00468 0.19 -3.5 512,207 - MIT1002_00468 0.22 -2.9 512,207 - MIT1002_00468 0.22 -4.8 512,289 - MIT1002_00468 0.29 -1.1 512,355 + MIT1002_00468 0.35 -1.1 512,375 + MIT1002_00468 0.36 -1.9 512,375 + MIT1002_00468 0.36 -1.4 512,383 - MIT1002_00468 0.37 -5.8 512,383 - MIT1002_00468 0.37 -0.4 512,383 - MIT1002_00468 0.37 -2.5 512,383 - MIT1002_00468 0.37 -3.3 512,383 - MIT1002_00468 0.37 -4.5 512,489 + MIT1002_00468 0.46 -2.8 512,490 - MIT1002_00468 0.46 -2.6 512,494 + MIT1002_00468 0.46 +0.1 512,497 - MIT1002_00468 0.46 -1.5 512,499 + MIT1002_00468 0.47 -2.8 512,502 - MIT1002_00468 0.47 -4.6 512,529 + MIT1002_00468 0.49 -2.4 512,532 - MIT1002_00468 0.49 -3.6 512,537 - MIT1002_00468 0.50 -1.2 512,599 - MIT1002_00468 0.55 -4.4 512,710 + MIT1002_00468 0.64 -2.4 512,726 + MIT1002_00468 0.65 -1.4 512,726 + MIT1002_00468 0.65 -2.6 512,777 - MIT1002_00468 0.69 -0.8 512,835 - MIT1002_00468 0.74 -3.4 512,866 + MIT1002_00468 0.77 -4.5 512,879 + MIT1002_00468 0.78 -2.5 512,888 - MIT1002_00468 0.79 -8.1 512,888 - MIT1002_00468 0.79 -3.2 512,912 - MIT1002_00468 0.81 -1.2 512,922 + MIT1002_00468 0.81 -2.2 512,930 - MIT1002_00468 0.82 -3.4 513,016 + MIT1002_00468 0.89 -1.8 513,029 - -1.4 513,107 + -4.3 513,112 + -2.5 513,112 + -2.6 513,120 - -4.0 513,232 + -4.4 513,308 + MIT1002_00469 0.14 -5.4 513,326 - MIT1002_00469 0.16 -3.2 513,356 + MIT1002_00469 0.19 -2.6 513,356 + MIT1002_00469 0.19 -4.6 513,393 + MIT1002_00469 0.23 -1.6 513,401 - MIT1002_00469 0.24 -2.4 513,439 + MIT1002_00469 0.28 -0.2 513,439 + MIT1002_00469 0.28 -3.2 513,447 - MIT1002_00469 0.29 -2.2 513,447 - MIT1002_00469 0.29 -5.1 513,461 + MIT1002_00469 0.30 -3.5 513,461 + MIT1002_00469 0.30 -4.2 513,461 + MIT1002_00469 0.30 -1.2 513,469 - MIT1002_00469 0.31 -2.3 513,469 - MIT1002_00469 0.31 -4.3 513,552 + MIT1002_00469 0.40 -3.0 513,552 + MIT1002_00469 0.40 -3.6 513,552 + MIT1002_00469 0.40 -3.3 513,552 + MIT1002_00469 0.40 -4.5 513,560 - MIT1002_00469 0.41 -2.8 513,580 + MIT1002_00469 0.43 -2.2 513,620 - MIT1002_00469 0.48 -3.3 513,635 + MIT1002_00469 0.49 -3.0 513,646 + MIT1002_00469 0.51 -3.1 513,653 - MIT1002_00469 0.51 -1.9 513,654 - MIT1002_00469 0.52 -1.3 513,700 + MIT1002_00469 0.57 -4.6 513,806 + MIT1002_00469 0.68 -3.0 513,833 - MIT1002_00469 0.71 -2.2 513,881 + MIT1002_00469 0.76 -3.6 513,887 - MIT1002_00469 0.77 -3.1 513,887 - MIT1002_00469 0.77 -3.2 513,887 - MIT1002_00469 0.77 -3.3 513,928 + MIT1002_00469 0.82 -1.3 513,963 + MIT1002_00469 0.85 -3.4 514,068 + -2.6 514,075 + -2.5 514,075 + +0.3 514,083 - -3.4 514,083 - -0.4 514,083 - -2.8 514,083 - -2.4 514,083 - -0.4 514,083 - -4.3 514,083 - -5.1 514,089 + -6.3 514,134 + -3.7 514,134 + -5.2 514,142 - -0.2
Or see this region's nucleotide sequence