Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00222 and MIT1002_00223 are separated by 36 nucleotides MIT1002_00223 and MIT1002_00224 overlap by 4 nucleotides MIT1002_00224 and MIT1002_00225 are separated by 26 nucleotides
MIT1002_00222: MIT1002_00222 - Rod shape-determining protein MreD, at 245,777 to 246,253
_00222
MIT1002_00223: MIT1002_00223 - Maf-like protein YhdE, at 246,290 to 246,895
_00223
MIT1002_00224: MIT1002_00224 - Ribonuclease G, at 246,892 to 248,358
_00224
MIT1002_00225: MIT1002_00225 - putative protein involved in outer membrane biogenesis, at 248,385 to 252,380
_00225
Position (kb)
246
247
248
249 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 246.191 kb on - strand, within MIT1002_00222 at 246.313 kb on - strand at 246.363 kb on + strand, within MIT1002_00223 at 246.363 kb on + strand, within MIT1002_00223 at 246.395 kb on + strand, within MIT1002_00223 at 246.431 kb on + strand, within MIT1002_00223 at 246.469 kb on + strand, within MIT1002_00223 at 246.501 kb on + strand, within MIT1002_00223 at 246.509 kb on - strand, within MIT1002_00223 at 246.522 kb on + strand, within MIT1002_00223 at 246.665 kb on + strand, within MIT1002_00223 at 246.729 kb on + strand, within MIT1002_00223 at 246.749 kb on + strand, within MIT1002_00223 at 246.762 kb on - strand, within MIT1002_00223 at 246.772 kb on - strand, within MIT1002_00223 at 246.772 kb on - strand, within MIT1002_00223 at 246.776 kb on + strand, within MIT1002_00223 at 246.806 kb on + strand, within MIT1002_00223 at 246.807 kb on + strand, within MIT1002_00223 at 246.825 kb on + strand, within MIT1002_00223 at 246.861 kb on + strand at 246.869 kb on - strand at 246.869 kb on - strand at 246.896 kb on + strand at 246.904 kb on - strand at 246.954 kb on - strand at 247.203 kb on + strand, within MIT1002_00224 at 247.238 kb on + strand, within MIT1002_00224 at 247.245 kb on + strand, within MIT1002_00224 at 247.245 kb on + strand, within MIT1002_00224 at 247.253 kb on - strand, within MIT1002_00224 at 247.272 kb on - strand, within MIT1002_00224 at 247.292 kb on - strand, within MIT1002_00224 at 247.562 kb on + strand, within MIT1002_00224 at 247.800 kb on + strand, within MIT1002_00224 at 247.822 kb on - strand, within MIT1002_00224 at 247.966 kb on + strand, within MIT1002_00224 at 247.976 kb on + strand, within MIT1002_00224 at 247.976 kb on + strand, within MIT1002_00224 at 247.976 kb on + strand, within MIT1002_00224 at 247.984 kb on - strand, within MIT1002_00224 at 247.984 kb on - strand, within MIT1002_00224 at 247.984 kb on - strand, within MIT1002_00224 at 248.024 kb on + strand, within MIT1002_00224 at 248.076 kb on - strand, within MIT1002_00224 at 248.091 kb on + strand, within MIT1002_00224 at 248.116 kb on + strand, within MIT1002_00224 at 248.116 kb on + strand, within MIT1002_00224 at 248.136 kb on - strand, within MIT1002_00224 at 248.247 kb on - strand at 248.368 kb on - strand at 248.469 kb on + strand at 248.487 kb on - strand at 248.520 kb on - strand at 248.551 kb on + strand at 248.589 kb on - strand at 248.610 kb on + strand at 248.680 kb on - strand at 248.739 kb on - strand at 248.739 kb on - strand at 248.741 kb on + strand at 248.746 kb on + strand at 248.814 kb on + strand, within MIT1002_00225 at 248.815 kb on + strand, within MIT1002_00225 at 248.823 kb on - strand, within MIT1002_00225 at 248.840 kb on + strand, within MIT1002_00225 at 248.883 kb on - strand, within MIT1002_00225 at 248.951 kb on + strand, within MIT1002_00225 at 248.959 kb on - strand, within MIT1002_00225 at 248.963 kb on + strand, within MIT1002_00225 at 248.963 kb on + strand, within MIT1002_00225 at 248.963 kb on + strand, within MIT1002_00225 at 248.971 kb on - strand, within MIT1002_00225 at 248.971 kb on - strand, within MIT1002_00225 at 248.976 kb on + strand, within MIT1002_00225 at 249.040 kb on - strand, within MIT1002_00225 at 249.063 kb on - strand, within MIT1002_00225 at 249.065 kb on + strand, within MIT1002_00225 at 249.117 kb on + strand, within MIT1002_00225 at 249.172 kb on + strand, within MIT1002_00225 at 249.189 kb on - strand, within MIT1002_00225 at 249.284 kb on - strand, within MIT1002_00225 at 249.344 kb on + strand, within MIT1002_00225 at 249.344 kb on + strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225 at 249.352 kb on - strand, within MIT1002_00225
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 246,191 - MIT1002_00222 0.87 -1.8 246,313 - +0.1 246,363 + MIT1002_00223 0.12 -0.6 246,363 + MIT1002_00223 0.12 -1.1 246,395 + MIT1002_00223 0.17 -0.8 246,431 + MIT1002_00223 0.23 +0.2 246,469 + MIT1002_00223 0.30 -0.2 246,501 + MIT1002_00223 0.35 -1.5 246,509 - MIT1002_00223 0.36 -1.6 246,522 + MIT1002_00223 0.38 +0.0 246,665 + MIT1002_00223 0.62 -0.4 246,729 + MIT1002_00223 0.72 -2.2 246,749 + MIT1002_00223 0.76 +0.7 246,762 - MIT1002_00223 0.78 -2.3 246,772 - MIT1002_00223 0.80 -1.4 246,772 - MIT1002_00223 0.80 -0.3 246,776 + MIT1002_00223 0.80 -0.2 246,806 + MIT1002_00223 0.85 +0.5 246,807 + MIT1002_00223 0.85 -0.7 246,825 + MIT1002_00223 0.88 +1.4 246,861 + +2.1 246,869 - -1.0 246,869 - -1.2 246,896 + -1.3 246,904 - +0.7 246,954 - -0.1 247,203 + MIT1002_00224 0.21 -1.9 247,238 + MIT1002_00224 0.24 -0.1 247,245 + MIT1002_00224 0.24 +0.4 247,245 + MIT1002_00224 0.24 -1.1 247,253 - MIT1002_00224 0.25 +0.4 247,272 - MIT1002_00224 0.26 -1.2 247,292 - MIT1002_00224 0.27 -0.1 247,562 + MIT1002_00224 0.46 +0.8 247,800 + MIT1002_00224 0.62 -2.6 247,822 - MIT1002_00224 0.63 -1.6 247,966 + MIT1002_00224 0.73 -1.1 247,976 + MIT1002_00224 0.74 -1.3 247,976 + MIT1002_00224 0.74 +0.3 247,976 + MIT1002_00224 0.74 -1.3 247,984 - MIT1002_00224 0.74 -1.5 247,984 - MIT1002_00224 0.74 +1.1 247,984 - MIT1002_00224 0.74 -0.1 248,024 + MIT1002_00224 0.77 -0.3 248,076 - MIT1002_00224 0.81 -1.6 248,091 + MIT1002_00224 0.82 -2.1 248,116 + MIT1002_00224 0.83 -2.3 248,116 + MIT1002_00224 0.83 -1.1 248,136 - MIT1002_00224 0.85 +1.5 248,247 - -1.0 248,368 - -0.8 248,469 + +3.0 248,487 - -0.2 248,520 - -0.4 248,551 + +0.1 248,589 - -1.1 248,610 + -0.1 248,680 - -1.4 248,739 - +0.7 248,739 - +0.8 248,741 + +0.2 248,746 + +1.1 248,814 + MIT1002_00225 0.11 +0.7 248,815 + MIT1002_00225 0.11 -1.1 248,823 - MIT1002_00225 0.11 +0.1 248,840 + MIT1002_00225 0.11 -0.8 248,883 - MIT1002_00225 0.12 -1.5 248,951 + MIT1002_00225 0.14 -0.1 248,959 - MIT1002_00225 0.14 +0.5 248,963 + MIT1002_00225 0.14 +0.7 248,963 + MIT1002_00225 0.14 -0.8 248,963 + MIT1002_00225 0.14 +0.0 248,971 - MIT1002_00225 0.15 +0.0 248,971 - MIT1002_00225 0.15 -0.4 248,976 + MIT1002_00225 0.15 -0.9 249,040 - MIT1002_00225 0.16 +0.3 249,063 - MIT1002_00225 0.17 -0.8 249,065 + MIT1002_00225 0.17 -1.2 249,117 + MIT1002_00225 0.18 -0.7 249,172 + MIT1002_00225 0.20 -1.2 249,189 - MIT1002_00225 0.20 -0.3 249,284 - MIT1002_00225 0.22 -0.8 249,344 + MIT1002_00225 0.24 -0.0 249,344 + MIT1002_00225 0.24 -0.4 249,352 - MIT1002_00225 0.24 -0.6 249,352 - MIT1002_00225 0.24 -0.5 249,352 - MIT1002_00225 0.24 -0.8 249,352 - MIT1002_00225 0.24 -1.3 249,352 - MIT1002_00225 0.24 -1.1
Or see this region's nucleotide sequence