Experiment: monoculture; Experiment B, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00096 and MIT1002_00097 overlap by 4 nucleotides MIT1002_00097 and MIT1002_00099 are separated by 494 nucleotides
MIT1002_00096: MIT1002_00096 - Thiamine-phosphate synthase, at 102,988 to 104,589
_00096
MIT1002_00097: MIT1002_00097 - Phosphomethylpyrimidine synthase, at 104,586 to 106,496
_00097
MIT1002_00099: MIT1002_00099 - putative protoheme IX biogenesis protein, at 106,991 to 108,166
_00099
Position (kb) 104
105
106
107 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 103.604 kb on - strand, within MIT1002_00096 at 103.683 kb on + strand, within MIT1002_00096 at 103.691 kb on - strand, within MIT1002_00096 at 103.698 kb on + strand, within MIT1002_00096 at 103.713 kb on - strand, within MIT1002_00096 at 103.735 kb on - strand, within MIT1002_00096 at 103.745 kb on - strand, within MIT1002_00096 at 103.803 kb on + strand, within MIT1002_00096 at 103.811 kb on - strand, within MIT1002_00096 at 103.818 kb on - strand, within MIT1002_00096 at 103.850 kb on - strand, within MIT1002_00096 at 103.850 kb on - strand, within MIT1002_00096 at 103.850 kb on - strand, within MIT1002_00096 at 103.897 kb on + strand, within MIT1002_00096 at 103.897 kb on + strand, within MIT1002_00096 at 103.897 kb on + strand, within MIT1002_00096 at 103.897 kb on + strand, within MIT1002_00096 at 103.897 kb on + strand, within MIT1002_00096 at 103.903 kb on + strand, within MIT1002_00096 at 103.903 kb on + strand, within MIT1002_00096 at 103.905 kb on - strand, within MIT1002_00096 at 103.905 kb on - strand, within MIT1002_00096 at 103.911 kb on - strand, within MIT1002_00096 at 103.961 kb on - strand, within MIT1002_00096 at 104.043 kb on - strand, within MIT1002_00096 at 104.050 kb on - strand, within MIT1002_00096 at 104.120 kb on - strand, within MIT1002_00096 at 104.224 kb on + strand, within MIT1002_00096 at 104.224 kb on + strand, within MIT1002_00096 at 104.225 kb on - strand, within MIT1002_00096 at 104.225 kb on - strand, within MIT1002_00096 at 104.273 kb on + strand, within MIT1002_00096 at 104.275 kb on + strand, within MIT1002_00096 at 104.418 kb on + strand, within MIT1002_00096 at 104.418 kb on - strand, within MIT1002_00096 at 104.424 kb on + strand, within MIT1002_00096 at 104.424 kb on + strand, within MIT1002_00096 at 104.424 kb on + strand, within MIT1002_00096 at 104.424 kb on + strand, within MIT1002_00096 at 104.424 kb on + strand, within MIT1002_00096 at 104.424 kb on + strand, within MIT1002_00096 at 104.430 kb on - strand at 104.432 kb on - strand at 104.432 kb on - strand at 104.443 kb on + strand at 104.474 kb on - strand at 104.506 kb on - strand at 104.513 kb on - strand at 104.543 kb on + strand at 104.551 kb on - strand at 104.554 kb on + strand at 104.572 kb on - strand at 104.593 kb on + strand at 104.604 kb on + strand at 104.624 kb on + strand at 104.624 kb on + strand at 104.629 kb on + strand at 104.629 kb on + strand at 104.637 kb on - strand at 104.786 kb on - strand, within MIT1002_00097 at 104.833 kb on - strand, within MIT1002_00097 at 104.835 kb on - strand, within MIT1002_00097 at 104.845 kb on + strand, within MIT1002_00097 at 104.964 kb on + strand, within MIT1002_00097 at 105.009 kb on + strand, within MIT1002_00097 at 105.017 kb on + strand, within MIT1002_00097 at 105.017 kb on + strand, within MIT1002_00097 at 105.017 kb on + strand, within MIT1002_00097 at 105.017 kb on + strand, within MIT1002_00097 at 105.017 kb on - strand, within MIT1002_00097 at 105.020 kb on + strand, within MIT1002_00097 at 105.025 kb on - strand, within MIT1002_00097 at 105.025 kb on - strand, within MIT1002_00097 at 105.035 kb on - strand, within MIT1002_00097 at 105.050 kb on - strand, within MIT1002_00097 at 105.053 kb on - strand, within MIT1002_00097 at 105.066 kb on + strand, within MIT1002_00097 at 105.067 kb on - strand, within MIT1002_00097 at 105.074 kb on - strand, within MIT1002_00097 at 105.119 kb on + strand, within MIT1002_00097 at 105.119 kb on + strand, within MIT1002_00097 at 105.127 kb on + strand, within MIT1002_00097 at 105.151 kb on + strand, within MIT1002_00097 at 105.229 kb on + strand, within MIT1002_00097 at 105.265 kb on - strand, within MIT1002_00097 at 105.292 kb on + strand, within MIT1002_00097 at 105.295 kb on - strand, within MIT1002_00097 at 105.316 kb on + strand, within MIT1002_00097 at 105.331 kb on - strand, within MIT1002_00097 at 105.331 kb on - strand, within MIT1002_00097 at 105.366 kb on - strand, within MIT1002_00097 at 105.399 kb on + strand, within MIT1002_00097 at 105.413 kb on + strand, within MIT1002_00097 at 105.478 kb on - strand, within MIT1002_00097 at 105.483 kb on - strand, within MIT1002_00097 at 105.490 kb on - strand, within MIT1002_00097 at 105.490 kb on - strand, within MIT1002_00097 at 105.490 kb on - strand, within MIT1002_00097 at 105.524 kb on + strand, within MIT1002_00097 at 105.532 kb on - strand, within MIT1002_00097 at 105.533 kb on - strand, within MIT1002_00097 at 105.533 kb on - strand, within MIT1002_00097 at 105.534 kb on + strand, within MIT1002_00097 at 105.536 kb on + strand, within MIT1002_00097 at 105.548 kb on + strand, within MIT1002_00097 at 105.593 kb on + strand, within MIT1002_00097 at 105.641 kb on + strand, within MIT1002_00097 at 105.641 kb on + strand, within MIT1002_00097 at 105.697 kb on + strand, within MIT1002_00097 at 105.711 kb on + strand, within MIT1002_00097 at 105.736 kb on - strand, within MIT1002_00097 at 105.736 kb on - strand, within MIT1002_00097 at 105.784 kb on - strand, within MIT1002_00097 at 105.843 kb on - strand, within MIT1002_00097 at 105.874 kb on - strand, within MIT1002_00097 at 105.887 kb on - strand, within MIT1002_00097 at 105.898 kb on - strand, within MIT1002_00097 at 105.898 kb on - strand, within MIT1002_00097 at 105.934 kb on + strand, within MIT1002_00097 at 105.949 kb on + strand, within MIT1002_00097 at 105.949 kb on + strand, within MIT1002_00097 at 105.949 kb on + strand, within MIT1002_00097 at 105.950 kb on - strand, within MIT1002_00097 at 105.957 kb on - strand, within MIT1002_00097 at 105.957 kb on - strand, within MIT1002_00097 at 105.957 kb on - strand, within MIT1002_00097 at 105.957 kb on - strand, within MIT1002_00097 at 105.962 kb on - strand, within MIT1002_00097 at 105.976 kb on - strand, within MIT1002_00097 at 105.977 kb on + strand, within MIT1002_00097 at 106.041 kb on + strand, within MIT1002_00097 at 106.046 kb on + strand, within MIT1002_00097 at 106.046 kb on + strand, within MIT1002_00097 at 106.046 kb on + strand, within MIT1002_00097 at 106.054 kb on - strand, within MIT1002_00097 at 106.054 kb on - strand, within MIT1002_00097 at 106.054 kb on - strand, within MIT1002_00097 at 106.054 kb on - strand, within MIT1002_00097 at 106.054 kb on - strand, within MIT1002_00097 at 106.159 kb on - strand, within MIT1002_00097 at 106.184 kb on - strand, within MIT1002_00097 at 106.227 kb on + strand, within MIT1002_00097 at 106.227 kb on + strand, within MIT1002_00097 at 106.227 kb on + strand, within MIT1002_00097 at 106.234 kb on - strand, within MIT1002_00097 at 106.235 kb on - strand, within MIT1002_00097 at 106.235 kb on - strand, within MIT1002_00097 at 106.252 kb on - strand, within MIT1002_00097 at 106.274 kb on + strand, within MIT1002_00097 at 106.274 kb on + strand, within MIT1002_00097 at 106.274 kb on + strand, within MIT1002_00097 at 106.275 kb on - strand, within MIT1002_00097 at 106.282 kb on - strand, within MIT1002_00097 at 106.282 kb on - strand, within MIT1002_00097 at 106.284 kb on + strand, within MIT1002_00097 at 106.292 kb on - strand, within MIT1002_00097 at 106.340 kb on + strand at 106.439 kb on - strand at 106.476 kb on + strand at 106.486 kb on - strand at 106.542 kb on - strand at 106.547 kb on - strand at 106.548 kb on - strand at 106.559 kb on + strand at 106.567 kb on - strand at 106.573 kb on - strand at 106.575 kb on + strand at 106.575 kb on + strand at 106.575 kb on + strand at 106.583 kb on - strand at 106.583 kb on - strand at 106.583 kb on - strand at 106.619 kb on - strand at 106.628 kb on - strand at 106.629 kb on + strand at 106.635 kb on - strand at 106.637 kb on - strand at 106.677 kb on - strand at 106.702 kb on - strand at 106.757 kb on + strand at 106.757 kb on + strand at 106.807 kb on - strand at 106.807 kb on - strand at 106.807 kb on - strand at 106.809 kb on + strand at 107.003 kb on + strand at 107.109 kb on + strand, within MIT1002_00099 at 107.109 kb on + strand, within MIT1002_00099 at 107.117 kb on - strand, within MIT1002_00099 at 107.170 kb on + strand, within MIT1002_00099 at 107.224 kb on + strand, within MIT1002_00099 at 107.224 kb on + strand, within MIT1002_00099 at 107.224 kb on + strand, within MIT1002_00099 at 107.224 kb on + strand, within MIT1002_00099 at 107.224 kb on + strand, within MIT1002_00099 at 107.232 kb on - strand, within MIT1002_00099 at 107.232 kb on - strand, within MIT1002_00099 at 107.232 kb on - strand, within MIT1002_00099 at 107.232 kb on - strand, within MIT1002_00099 at 107.232 kb on - strand, within MIT1002_00099 at 107.250 kb on + strand, within MIT1002_00099 at 107.258 kb on - strand, within MIT1002_00099 at 107.360 kb on + strand, within MIT1002_00099 at 107.360 kb on + strand, within MIT1002_00099 at 107.377 kb on + strand, within MIT1002_00099 at 107.403 kb on - strand, within MIT1002_00099 at 107.426 kb on + strand, within MIT1002_00099 at 107.485 kb on + strand, within MIT1002_00099 at 107.486 kb on + strand, within MIT1002_00099
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment B, time point 2 remove 103,604 - MIT1002_00096 0.38 -2.0 103,683 + MIT1002_00096 0.43 -4.0 103,691 - MIT1002_00096 0.44 -0.8 103,698 + MIT1002_00096 0.44 -0.6 103,713 - MIT1002_00096 0.45 -1.9 103,735 - MIT1002_00096 0.47 -2.5 103,745 - MIT1002_00096 0.47 -2.5 103,803 + MIT1002_00096 0.51 -2.7 103,811 - MIT1002_00096 0.51 -3.6 103,818 - MIT1002_00096 0.52 -1.3 103,850 - MIT1002_00096 0.54 -0.1 103,850 - MIT1002_00096 0.54 -0.8 103,850 - MIT1002_00096 0.54 -2.3 103,897 + MIT1002_00096 0.57 -1.9 103,897 + MIT1002_00096 0.57 -2.2 103,897 + MIT1002_00096 0.57 -1.2 103,897 + MIT1002_00096 0.57 -2.7 103,897 + MIT1002_00096 0.57 -3.5 103,903 + MIT1002_00096 0.57 -3.2 103,903 + MIT1002_00096 0.57 -2.0 103,905 - MIT1002_00096 0.57 +1.7 103,905 - MIT1002_00096 0.57 -3.3 103,911 - MIT1002_00096 0.58 +0.4 103,961 - MIT1002_00096 0.61 -2.8 104,043 - MIT1002_00096 0.66 +0.1 104,050 - MIT1002_00096 0.66 -3.5 104,120 - MIT1002_00096 0.71 -1.8 104,224 + MIT1002_00096 0.77 -5.5 104,224 + MIT1002_00096 0.77 -1.2 104,225 - MIT1002_00096 0.77 -1.9 104,225 - MIT1002_00096 0.77 +0.1 104,273 + MIT1002_00096 0.80 -2.6 104,275 + MIT1002_00096 0.80 -3.2 104,418 + MIT1002_00096 0.89 -2.3 104,418 - MIT1002_00096 0.89 -2.4 104,424 + MIT1002_00096 0.90 -2.0 104,424 + MIT1002_00096 0.90 -2.9 104,424 + MIT1002_00096 0.90 -0.1 104,424 + MIT1002_00096 0.90 -1.7 104,424 + MIT1002_00096 0.90 -1.5 104,424 + MIT1002_00096 0.90 -3.7 104,430 - -2.2 104,432 - +0.4 104,432 - -3.7 104,443 + -0.1 104,474 - -2.2 104,506 - -2.1 104,513 - -2.5 104,543 + -3.0 104,551 - -4.4 104,554 + -1.0 104,572 - -2.9 104,593 + -2.7 104,604 + -1.6 104,624 + -3.7 104,624 + -3.1 104,629 + -3.4 104,629 + -1.6 104,637 - -1.9 104,786 - MIT1002_00097 0.10 -2.7 104,833 - MIT1002_00097 0.13 -2.5 104,835 - MIT1002_00097 0.13 -2.6 104,845 + MIT1002_00097 0.14 -1.5 104,964 + MIT1002_00097 0.20 -2.7 105,009 + MIT1002_00097 0.22 -2.5 105,017 + MIT1002_00097 0.23 -5.2 105,017 + MIT1002_00097 0.23 -2.7 105,017 + MIT1002_00097 0.23 -1.9 105,017 + MIT1002_00097 0.23 -5.1 105,017 - MIT1002_00097 0.23 -4.7 105,020 + MIT1002_00097 0.23 -1.3 105,025 - MIT1002_00097 0.23 -2.5 105,025 - MIT1002_00097 0.23 -2.7 105,035 - MIT1002_00097 0.23 -3.6 105,050 - MIT1002_00097 0.24 -0.2 105,053 - MIT1002_00097 0.24 -2.8 105,066 + MIT1002_00097 0.25 -1.3 105,067 - MIT1002_00097 0.25 -2.8 105,074 - MIT1002_00097 0.26 -4.5 105,119 + MIT1002_00097 0.28 -2.6 105,119 + MIT1002_00097 0.28 -1.6 105,127 + MIT1002_00097 0.28 +0.7 105,151 + MIT1002_00097 0.30 -0.6 105,229 + MIT1002_00097 0.34 -1.5 105,265 - MIT1002_00097 0.36 -2.7 105,292 + MIT1002_00097 0.37 -0.7 105,295 - MIT1002_00097 0.37 -2.0 105,316 + MIT1002_00097 0.38 -0.2 105,331 - MIT1002_00097 0.39 -3.2 105,331 - MIT1002_00097 0.39 -1.4 105,366 - MIT1002_00097 0.41 -2.6 105,399 + MIT1002_00097 0.43 -2.7 105,413 + MIT1002_00097 0.43 -2.2 105,478 - MIT1002_00097 0.47 -2.6 105,483 - MIT1002_00097 0.47 -3.3 105,490 - MIT1002_00097 0.47 -1.9 105,490 - MIT1002_00097 0.47 -3.5 105,490 - MIT1002_00097 0.47 -3.5 105,524 + MIT1002_00097 0.49 -3.6 105,532 - MIT1002_00097 0.50 -3.8 105,533 - MIT1002_00097 0.50 -1.9 105,533 - MIT1002_00097 0.50 -2.4 105,534 + MIT1002_00097 0.50 -0.3 105,536 + MIT1002_00097 0.50 -3.4 105,548 + MIT1002_00097 0.50 -4.2 105,593 + MIT1002_00097 0.53 -0.6 105,641 + MIT1002_00097 0.55 -2.8 105,641 + MIT1002_00097 0.55 -1.7 105,697 + MIT1002_00097 0.58 -2.5 105,711 + MIT1002_00097 0.59 -4.0 105,736 - MIT1002_00097 0.60 +0.4 105,736 - MIT1002_00097 0.60 -4.4 105,784 - MIT1002_00097 0.63 -1.9 105,843 - MIT1002_00097 0.66 -3.3 105,874 - MIT1002_00097 0.67 -1.7 105,887 - MIT1002_00097 0.68 -4.1 105,898 - MIT1002_00097 0.69 -3.6 105,898 - MIT1002_00097 0.69 -1.9 105,934 + MIT1002_00097 0.71 -3.3 105,949 + MIT1002_00097 0.71 -2.2 105,949 + MIT1002_00097 0.71 -0.1 105,949 + MIT1002_00097 0.71 -2.0 105,950 - MIT1002_00097 0.71 +0.1 105,957 - MIT1002_00097 0.72 -2.6 105,957 - MIT1002_00097 0.72 -0.3 105,957 - MIT1002_00097 0.72 -2.7 105,957 - MIT1002_00097 0.72 -2.1 105,962 - MIT1002_00097 0.72 -0.6 105,976 - MIT1002_00097 0.73 -1.5 105,977 + MIT1002_00097 0.73 -2.4 106,041 + MIT1002_00097 0.76 -1.4 106,046 + MIT1002_00097 0.76 -4.1 106,046 + MIT1002_00097 0.76 -2.0 106,046 + MIT1002_00097 0.76 -3.3 106,054 - MIT1002_00097 0.77 -0.8 106,054 - MIT1002_00097 0.77 -2.6 106,054 - MIT1002_00097 0.77 -5.6 106,054 - MIT1002_00097 0.77 -1.9 106,054 - MIT1002_00097 0.77 -2.2 106,159 - MIT1002_00097 0.82 -2.9 106,184 - MIT1002_00097 0.84 -2.7 106,227 + MIT1002_00097 0.86 -2.5 106,227 + MIT1002_00097 0.86 -2.9 106,227 + MIT1002_00097 0.86 -1.7 106,234 - MIT1002_00097 0.86 -2.7 106,235 - MIT1002_00097 0.86 -1.3 106,235 - MIT1002_00097 0.86 -2.1 106,252 - MIT1002_00097 0.87 -1.0 106,274 + MIT1002_00097 0.88 -1.2 106,274 + MIT1002_00097 0.88 -5.0 106,274 + MIT1002_00097 0.88 -1.5 106,275 - MIT1002_00097 0.88 -0.9 106,282 - MIT1002_00097 0.89 -2.3 106,282 - MIT1002_00097 0.89 -3.8 106,284 + MIT1002_00097 0.89 -2.3 106,292 - MIT1002_00097 0.89 -4.4 106,340 + -3.0 106,439 - -3.2 106,476 + -2.9 106,486 - -3.0 106,542 - -3.9 106,547 - -3.0 106,548 - -1.6 106,559 + -0.3 106,567 - -2.8 106,573 - -4.4 106,575 + -2.6 106,575 + -0.8 106,575 + -3.0 106,583 - -4.4 106,583 - -0.5 106,583 - -2.2 106,619 - -1.8 106,628 - -4.9 106,629 + -3.1 106,635 - -1.9 106,637 - -3.6 106,677 - -1.0 106,702 - -2.2 106,757 + -3.5 106,757 + -2.4 106,807 - -3.1 106,807 - -4.8 106,807 - -2.8 106,809 + +1.3 107,003 + +0.5 107,109 + MIT1002_00099 0.10 +0.7 107,109 + MIT1002_00099 0.10 +1.0 107,117 - MIT1002_00099 0.11 +1.3 107,170 + MIT1002_00099 0.15 +2.4 107,224 + MIT1002_00099 0.20 +1.2 107,224 + MIT1002_00099 0.20 -0.3 107,224 + MIT1002_00099 0.20 +0.1 107,224 + MIT1002_00099 0.20 -0.9 107,224 + MIT1002_00099 0.20 +1.5 107,232 - MIT1002_00099 0.20 -2.5 107,232 - MIT1002_00099 0.20 -2.0 107,232 - MIT1002_00099 0.20 +1.6 107,232 - MIT1002_00099 0.20 +0.2 107,232 - MIT1002_00099 0.20 -0.0 107,250 + MIT1002_00099 0.22 -2.8 107,258 - MIT1002_00099 0.23 -2.4 107,360 + MIT1002_00099 0.31 -0.1 107,360 + MIT1002_00099 0.31 -0.4 107,377 + MIT1002_00099 0.33 -2.2 107,403 - MIT1002_00099 0.35 +1.3 107,426 + MIT1002_00099 0.37 +1.3 107,485 + MIT1002_00099 0.42 +0.4 107,486 + MIT1002_00099 0.42 -0.1
Or see this region's nucleotide sequence