Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS32805 and QEN71_RS32810 are separated by 139 nucleotides QEN71_RS32810 and QEN71_RS32815 are separated by 134 nucleotides QEN71_RS32815 and QEN71_RS32820 overlap by 14 nucleotides QEN71_RS32820 and QEN71_RS32825 overlap by 4 nucleotides
QEN71_RS32805: QEN71_RS32805 - hypothetical protein, at 692,403 to 692,819
_RS32805
QEN71_RS32810: QEN71_RS32810 - formaldehyde-activating enzyme, at 692,959 to 693,468
_RS32810
QEN71_RS32815: QEN71_RS32815 - triphosphoribosyl-dephospho-CoA synthase, at 693,603 to 694,478
_RS32815
QEN71_RS32820: QEN71_RS32820 - RimK family alpha-L-glutamate ligase, at 694,465 to 695,448
_RS32820
QEN71_RS32825: QEN71_RS32825 - methenyltetrahydromethanopterin cyclohydrolase, at 695,445 to 696,548
_RS32825
Position (kb) 693
694
695 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 692.624 kb on - strand, within QEN71_RS32805 at 692.624 kb on - strand, within QEN71_RS32805 at 692.671 kb on + strand, within QEN71_RS32805 at 692.672 kb on - strand, within QEN71_RS32805 at 692.701 kb on + strand, within QEN71_RS32805 at 692.702 kb on - strand, within QEN71_RS32805 at 692.831 kb on + strand at 692.836 kb on + strand at 692.949 kb on + strand at 692.949 kb on + strand at 692.949 kb on + strand at 692.949 kb on + strand at 692.950 kb on - strand at 692.950 kb on - strand at 692.950 kb on - strand at 692.950 kb on - strand at 692.950 kb on - strand at 693.065 kb on + strand, within QEN71_RS32810 at 693.065 kb on + strand, within QEN71_RS32810 at 693.065 kb on + strand, within QEN71_RS32810 at 693.066 kb on - strand, within QEN71_RS32810 at 693.066 kb on - strand, within QEN71_RS32810 at 693.066 kb on - strand, within QEN71_RS32810 at 693.442 kb on + strand at 693.569 kb on - strand at 693.614 kb on + strand at 693.614 kb on + strand at 693.614 kb on + strand at 693.834 kb on + strand, within QEN71_RS32815 at 693.834 kb on + strand, within QEN71_RS32815 at 693.834 kb on + strand, within QEN71_RS32815 at 693.835 kb on - strand, within QEN71_RS32815 at 693.945 kb on - strand, within QEN71_RS32815 at 694.096 kb on + strand, within QEN71_RS32815 at 694.679 kb on + strand, within QEN71_RS32820 at 694.850 kb on + strand, within QEN71_RS32820 at 694.850 kb on + strand, within QEN71_RS32820 at 694.851 kb on - strand, within QEN71_RS32820 at 694.851 kb on - strand, within QEN71_RS32820 at 694.883 kb on + strand, within QEN71_RS32820 at 694.895 kb on + strand, within QEN71_RS32820 at 694.896 kb on - strand, within QEN71_RS32820 at 694.908 kb on + strand, within QEN71_RS32820 at 694.911 kb on - strand, within QEN71_RS32820 at 695.168 kb on + strand, within QEN71_RS32820 at 695.169 kb on - strand, within QEN71_RS32820 at 695.322 kb on + strand, within QEN71_RS32820 at 695.322 kb on + strand, within QEN71_RS32820 at 695.322 kb on + strand, within QEN71_RS32820 at 695.323 kb on - strand, within QEN71_RS32820 at 695.323 kb on - strand, within QEN71_RS32820 at 695.323 kb on - strand, within QEN71_RS32820 at 695.323 kb on - strand, within QEN71_RS32820 at 695.323 kb on - strand, within QEN71_RS32820
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 692,624 - QEN71_RS32805 0.53 +0.7 692,624 - QEN71_RS32805 0.53 -1.1 692,671 + QEN71_RS32805 0.64 -0.0 692,672 - QEN71_RS32805 0.65 -2.1 692,701 + QEN71_RS32805 0.71 -0.5 692,702 - QEN71_RS32805 0.72 -0.0 692,831 + +0.1 692,836 + +0.6 692,949 + -0.4 692,949 + -0.6 692,949 + -2.3 692,949 + +0.7 692,950 - -0.3 692,950 - -0.3 692,950 - +0.6 692,950 - +0.2 692,950 - -0.0 693,065 + QEN71_RS32810 0.21 +0.5 693,065 + QEN71_RS32810 0.21 +0.3 693,065 + QEN71_RS32810 0.21 +2.0 693,066 - QEN71_RS32810 0.21 -0.3 693,066 - QEN71_RS32810 0.21 -0.3 693,066 - QEN71_RS32810 0.21 -0.0 693,442 + -0.2 693,569 - -0.3 693,614 + -0.8 693,614 + -0.3 693,614 + +0.2 693,834 + QEN71_RS32815 0.26 -0.4 693,834 + QEN71_RS32815 0.26 +0.4 693,834 + QEN71_RS32815 0.26 -0.1 693,835 - QEN71_RS32815 0.26 -0.6 693,945 - QEN71_RS32815 0.39 -0.2 694,096 + QEN71_RS32815 0.56 -0.8 694,679 + QEN71_RS32820 0.22 -0.3 694,850 + QEN71_RS32820 0.39 -2.9 694,850 + QEN71_RS32820 0.39 -0.2 694,851 - QEN71_RS32820 0.39 -0.6 694,851 - QEN71_RS32820 0.39 +0.4 694,883 + QEN71_RS32820 0.42 +0.2 694,895 + QEN71_RS32820 0.44 -3.2 694,896 - QEN71_RS32820 0.44 -1.4 694,908 + QEN71_RS32820 0.45 -0.1 694,911 - QEN71_RS32820 0.45 -1.2 695,168 + QEN71_RS32820 0.71 -0.2 695,169 - QEN71_RS32820 0.72 +0.1 695,322 + QEN71_RS32820 0.87 -2.3 695,322 + QEN71_RS32820 0.87 -1.2 695,322 + QEN71_RS32820 0.87 -0.9 695,323 - QEN71_RS32820 0.87 -0.3 695,323 - QEN71_RS32820 0.87 -0.7 695,323 - QEN71_RS32820 0.87 -1.7 695,323 - QEN71_RS32820 0.87 +0.6 695,323 - QEN71_RS32820 0.87 -0.4
Or see this region's nucleotide sequence