Experiment: D-Sorbitol (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt QEN71_RS12390 and QEN71_RS12395 are separated by 610 nucleotides QEN71_RS12395 and QEN71_RS12400 are separated by 47 nucleotides
QEN71_RS12390: QEN71_RS12390 - 3-hydroxyisobutyrate dehydrogenase, at 2,776,372 to 2,777,265
_RS12390
QEN71_RS12395: QEN71_RS12395 - CoA-acylating methylmalonate-semialdehyde dehydrogenase, at 2,777,876 to 2,779,405
_RS12395
QEN71_RS12400: QEN71_RS12400 - AMP-binding protein, at 2,779,453 to 2,781,162
_RS12400
Position (kb)
2777
2778
2779
2780 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2776.993 kb on + strand, within QEN71_RS12390 at 2776.996 kb on + strand, within QEN71_RS12390 at 2776.996 kb on + strand, within QEN71_RS12390 at 2776.997 kb on - strand, within QEN71_RS12390 at 2776.997 kb on - strand, within QEN71_RS12390 at 2776.997 kb on - strand, within QEN71_RS12390 at 2777.015 kb on - strand, within QEN71_RS12390 at 2777.057 kb on - strand, within QEN71_RS12390 at 2777.057 kb on - strand, within QEN71_RS12390 at 2777.095 kb on + strand, within QEN71_RS12390 at 2777.095 kb on + strand, within QEN71_RS12390 at 2777.095 kb on + strand, within QEN71_RS12390 at 2777.248 kb on + strand at 2777.248 kb on + strand at 2777.249 kb on - strand at 2777.249 kb on - strand at 2777.249 kb on - strand at 2777.560 kb on + strand at 2777.560 kb on + strand at 2777.560 kb on + strand at 2777.561 kb on - strand at 2777.561 kb on - strand at 2777.561 kb on - strand at 2777.561 kb on - strand at 2777.561 kb on - strand at 2777.561 kb on - strand at 2777.561 kb on - strand at 2777.582 kb on + strand at 2777.649 kb on + strand at 2777.690 kb on - strand at 2777.856 kb on - strand at 2777.856 kb on - strand at 2777.893 kb on + strand at 2777.893 kb on + strand at 2777.893 kb on + strand at 2777.894 kb on - strand at 2777.894 kb on - strand at 2777.951 kb on - strand at 2777.951 kb on - strand at 2778.026 kb on - strand at 2778.055 kb on + strand, within QEN71_RS12395 at 2778.056 kb on - strand, within QEN71_RS12395 at 2778.056 kb on - strand, within QEN71_RS12395 at 2778.061 kb on + strand, within QEN71_RS12395 at 2778.062 kb on - strand, within QEN71_RS12395 at 2778.103 kb on + strand, within QEN71_RS12395 at 2778.340 kb on + strand, within QEN71_RS12395 at 2778.340 kb on + strand, within QEN71_RS12395 at 2778.380 kb on - strand, within QEN71_RS12395 at 2778.380 kb on - strand, within QEN71_RS12395 at 2778.425 kb on - strand, within QEN71_RS12395 at 2778.526 kb on + strand, within QEN71_RS12395 at 2778.526 kb on + strand, within QEN71_RS12395 at 2778.526 kb on + strand, within QEN71_RS12395 at 2778.669 kb on + strand, within QEN71_RS12395 at 2778.669 kb on + strand, within QEN71_RS12395 at 2778.669 kb on + strand, within QEN71_RS12395 at 2778.670 kb on - strand, within QEN71_RS12395 at 2778.670 kb on - strand, within QEN71_RS12395 at 2778.670 kb on - strand, within QEN71_RS12395 at 2778.670 kb on - strand, within QEN71_RS12395 at 2778.670 kb on - strand, within QEN71_RS12395 at 2778.719 kb on + strand, within QEN71_RS12395 at 2778.720 kb on - strand, within QEN71_RS12395 at 2778.720 kb on - strand, within QEN71_RS12395 at 2778.720 kb on - strand, within QEN71_RS12395 at 2778.720 kb on - strand, within QEN71_RS12395 at 2778.876 kb on - strand, within QEN71_RS12395 at 2778.876 kb on - strand, within QEN71_RS12395 at 2778.876 kb on - strand, within QEN71_RS12395 at 2778.969 kb on + strand, within QEN71_RS12395 at 2778.969 kb on + strand, within QEN71_RS12395 at 2778.969 kb on + strand, within QEN71_RS12395 at 2778.970 kb on - strand, within QEN71_RS12395 at 2779.066 kb on + strand, within QEN71_RS12395 at 2779.155 kb on + strand, within QEN71_RS12395 at 2779.155 kb on + strand, within QEN71_RS12395 at 2779.155 kb on + strand, within QEN71_RS12395 at 2779.156 kb on - strand, within QEN71_RS12395 at 2779.241 kb on + strand, within QEN71_RS12395 at 2779.242 kb on - strand, within QEN71_RS12395 at 2779.242 kb on - strand, within QEN71_RS12395 at 2779.454 kb on + strand at 2779.454 kb on + strand at 2779.454 kb on + strand at 2779.455 kb on - strand at 2779.455 kb on - strand at 2779.455 kb on - strand at 2779.499 kb on + strand at 2779.625 kb on + strand, within QEN71_RS12400 at 2779.626 kb on - strand, within QEN71_RS12400 at 2779.626 kb on - strand, within QEN71_RS12400 at 2779.626 kb on - strand, within QEN71_RS12400 at 2779.626 kb on - strand, within QEN71_RS12400 at 2779.859 kb on + strand, within QEN71_RS12400 at 2779.860 kb on - strand, within QEN71_RS12400 at 2779.868 kb on + strand, within QEN71_RS12400 at 2779.868 kb on + strand, within QEN71_RS12400 at 2779.868 kb on + strand, within QEN71_RS12400 at 2779.907 kb on + strand, within QEN71_RS12400 at 2779.907 kb on + strand, within QEN71_RS12400 at 2779.908 kb on - strand, within QEN71_RS12400 at 2779.908 kb on - strand, within QEN71_RS12400 at 2779.911 kb on - strand, within QEN71_RS12400 at 2779.956 kb on - strand, within QEN71_RS12400
Per-strain Table
Position Strand Gene LocusTag Fraction D-Sorbitol (C) remove 2,776,993 + QEN71_RS12390 0.69 -0.8 2,776,996 + QEN71_RS12390 0.70 +0.3 2,776,996 + QEN71_RS12390 0.70 -0.2 2,776,997 - QEN71_RS12390 0.70 -1.5 2,776,997 - QEN71_RS12390 0.70 -0.1 2,776,997 - QEN71_RS12390 0.70 +0.2 2,777,015 - QEN71_RS12390 0.72 -0.2 2,777,057 - QEN71_RS12390 0.77 -0.7 2,777,057 - QEN71_RS12390 0.77 -0.7 2,777,095 + QEN71_RS12390 0.81 -0.2 2,777,095 + QEN71_RS12390 0.81 -1.4 2,777,095 + QEN71_RS12390 0.81 +0.5 2,777,248 + +0.7 2,777,248 + +0.1 2,777,249 - -0.8 2,777,249 - -1.0 2,777,249 - -1.8 2,777,560 + -0.1 2,777,560 + -0.0 2,777,560 + +0.8 2,777,561 - +0.7 2,777,561 - -0.7 2,777,561 - +0.6 2,777,561 - +0.9 2,777,561 - +0.3 2,777,561 - +0.1 2,777,561 - -1.5 2,777,582 + +0.4 2,777,649 + +0.1 2,777,690 - +0.4 2,777,856 - -0.7 2,777,856 - +0.9 2,777,893 + -1.8 2,777,893 + +0.8 2,777,893 + +0.1 2,777,894 - -2.0 2,777,894 - -0.1 2,777,951 - -0.1 2,777,951 - -0.7 2,778,026 - +0.6 2,778,055 + QEN71_RS12395 0.12 +0.3 2,778,056 - QEN71_RS12395 0.12 -1.9 2,778,056 - QEN71_RS12395 0.12 +0.8 2,778,061 + QEN71_RS12395 0.12 +0.0 2,778,062 - QEN71_RS12395 0.12 -0.0 2,778,103 + QEN71_RS12395 0.15 -2.3 2,778,340 + QEN71_RS12395 0.30 +0.7 2,778,340 + QEN71_RS12395 0.30 -0.6 2,778,380 - QEN71_RS12395 0.33 -0.8 2,778,380 - QEN71_RS12395 0.33 -1.1 2,778,425 - QEN71_RS12395 0.36 -0.7 2,778,526 + QEN71_RS12395 0.42 -1.5 2,778,526 + QEN71_RS12395 0.42 -1.6 2,778,526 + QEN71_RS12395 0.42 +1.3 2,778,669 + QEN71_RS12395 0.52 -0.6 2,778,669 + QEN71_RS12395 0.52 +0.3 2,778,669 + QEN71_RS12395 0.52 -3.4 2,778,670 - QEN71_RS12395 0.52 -0.1 2,778,670 - QEN71_RS12395 0.52 -1.0 2,778,670 - QEN71_RS12395 0.52 +0.0 2,778,670 - QEN71_RS12395 0.52 +1.0 2,778,670 - QEN71_RS12395 0.52 +0.6 2,778,719 + QEN71_RS12395 0.55 +0.3 2,778,720 - QEN71_RS12395 0.55 -0.4 2,778,720 - QEN71_RS12395 0.55 -1.8 2,778,720 - QEN71_RS12395 0.55 +0.0 2,778,720 - QEN71_RS12395 0.55 +0.7 2,778,876 - QEN71_RS12395 0.65 -0.0 2,778,876 - QEN71_RS12395 0.65 -1.9 2,778,876 - QEN71_RS12395 0.65 -0.5 2,778,969 + QEN71_RS12395 0.71 +0.8 2,778,969 + QEN71_RS12395 0.71 -0.0 2,778,969 + QEN71_RS12395 0.71 +0.4 2,778,970 - QEN71_RS12395 0.72 +0.6 2,779,066 + QEN71_RS12395 0.78 -0.4 2,779,155 + QEN71_RS12395 0.84 +0.6 2,779,155 + QEN71_RS12395 0.84 +0.2 2,779,155 + QEN71_RS12395 0.84 +0.0 2,779,156 - QEN71_RS12395 0.84 +0.6 2,779,241 + QEN71_RS12395 0.89 -1.0 2,779,242 - QEN71_RS12395 0.89 -0.1 2,779,242 - QEN71_RS12395 0.89 -0.1 2,779,454 + -1.3 2,779,454 + -0.9 2,779,454 + -0.8 2,779,455 - +0.3 2,779,455 - +0.3 2,779,455 - +1.1 2,779,499 + -0.4 2,779,625 + QEN71_RS12400 0.10 -0.4 2,779,626 - QEN71_RS12400 0.10 -0.2 2,779,626 - QEN71_RS12400 0.10 -0.3 2,779,626 - QEN71_RS12400 0.10 -0.2 2,779,626 - QEN71_RS12400 0.10 +0.6 2,779,859 + QEN71_RS12400 0.24 -0.7 2,779,860 - QEN71_RS12400 0.24 -1.1 2,779,868 + QEN71_RS12400 0.24 -0.1 2,779,868 + QEN71_RS12400 0.24 -1.7 2,779,868 + QEN71_RS12400 0.24 -0.1 2,779,907 + QEN71_RS12400 0.27 -0.2 2,779,907 + QEN71_RS12400 0.27 +1.6 2,779,908 - QEN71_RS12400 0.27 -0.4 2,779,908 - QEN71_RS12400 0.27 -0.8 2,779,911 - QEN71_RS12400 0.27 -0.1 2,779,956 - QEN71_RS12400 0.29 +0.2
Or see this region's nucleotide sequence