Experiment: monoculture; Experiment A, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02180 and MIT1002_02181 are separated by 284 nucleotides MIT1002_02181 and MIT1002_02182 are separated by 36 nucleotides MIT1002_02182 and MIT1002_02183 overlap by 7 nucleotides MIT1002_02183 and MIT1002_02184 are separated by 115 nucleotides MIT1002_02184 and MIT1002_02185 are separated by 6 nucleotides
MIT1002_02180: MIT1002_02180 - DNA translocase FtsK, at 2,435,183 to 2,437,978
_02180
MIT1002_02181: MIT1002_02181 - Leucine-responsive regulatory protein, at 2,438,263 to 2,438,748
_02181
MIT1002_02182: MIT1002_02182 - hypothetical protein, at 2,438,785 to 2,438,940
_02182
MIT1002_02183: MIT1002_02183 - Alanine dehydrogenase, at 2,438,934 to 2,440,067
_02183
MIT1002_02184: MIT1002_02184 - Orotidine 5'-phosphate decarboxylase, at 2,440,183 to 2,440,881
_02184
MIT1002_02185: MIT1002_02185 - tetratricopeptide repeat protein, at 2,440,888 to 2,442,057
_02185
Position (kb)
2438
2439
2440
2441 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2438.059 kb on - strand at 2438.059 kb on - strand at 2438.059 kb on - strand at 2438.071 kb on - strand at 2438.168 kb on - strand at 2438.168 kb on - strand at 2438.168 kb on - strand at 2438.168 kb on - strand at 2438.234 kb on - strand at 2438.252 kb on - strand at 2438.342 kb on + strand, within MIT1002_02181 at 2438.347 kb on + strand, within MIT1002_02181 at 2438.450 kb on + strand, within MIT1002_02181 at 2438.499 kb on - strand, within MIT1002_02181 at 2438.558 kb on + strand, within MIT1002_02181 at 2438.590 kb on + strand, within MIT1002_02181 at 2438.590 kb on + strand, within MIT1002_02181 at 2438.598 kb on - strand, within MIT1002_02181 at 2438.627 kb on + strand, within MIT1002_02181 at 2438.913 kb on + strand, within MIT1002_02182 at 2438.928 kb on + strand at 2438.928 kb on + strand at 2438.928 kb on + strand at 2438.933 kb on - strand at 2439.161 kb on + strand, within MIT1002_02183 at 2439.195 kb on + strand, within MIT1002_02183 at 2439.204 kb on + strand, within MIT1002_02183 at 2439.204 kb on + strand, within MIT1002_02183 at 2439.204 kb on + strand, within MIT1002_02183 at 2439.211 kb on - strand, within MIT1002_02183 at 2439.212 kb on - strand, within MIT1002_02183 at 2439.212 kb on - strand, within MIT1002_02183 at 2439.212 kb on - strand, within MIT1002_02183 at 2439.212 kb on - strand, within MIT1002_02183 at 2439.212 kb on - strand, within MIT1002_02183 at 2439.239 kb on - strand, within MIT1002_02183 at 2439.272 kb on - strand, within MIT1002_02183 at 2439.357 kb on + strand, within MIT1002_02183 at 2439.385 kb on - strand, within MIT1002_02183 at 2439.435 kb on - strand, within MIT1002_02183 at 2439.465 kb on + strand, within MIT1002_02183 at 2439.606 kb on + strand, within MIT1002_02183 at 2439.619 kb on - strand, within MIT1002_02183 at 2439.648 kb on + strand, within MIT1002_02183 at 2439.656 kb on - strand, within MIT1002_02183 at 2439.686 kb on - strand, within MIT1002_02183 at 2439.703 kb on - strand, within MIT1002_02183 at 2439.763 kb on + strand, within MIT1002_02183 at 2439.780 kb on + strand, within MIT1002_02183 at 2439.783 kb on - strand, within MIT1002_02183 at 2439.788 kb on - strand, within MIT1002_02183 at 2439.788 kb on - strand, within MIT1002_02183 at 2439.788 kb on - strand, within MIT1002_02183 at 2439.818 kb on + strand, within MIT1002_02183 at 2439.958 kb on + strand at 2439.958 kb on + strand at 2439.989 kb on - strand at 2439.994 kb on - strand at 2439.994 kb on - strand at 2439.996 kb on + strand at 2440.038 kb on - strand at 2440.039 kb on - strand at 2440.059 kb on + strand at 2440.151 kb on - strand at 2440.181 kb on + strand at 2440.228 kb on + strand at 2440.228 kb on - strand at 2440.423 kb on + strand, within MIT1002_02184 at 2440.423 kb on + strand, within MIT1002_02184 at 2440.476 kb on + strand, within MIT1002_02184 at 2440.790 kb on - strand, within MIT1002_02184 at 2440.849 kb on + strand at 2440.880 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 6 remove 2,438,059 - -1.7 2,438,059 - -0.4 2,438,059 - -0.2 2,438,071 - +3.7 2,438,168 - -2.3 2,438,168 - +0.9 2,438,168 - -0.4 2,438,168 - +1.1 2,438,234 - -0.6 2,438,252 - -1.7 2,438,342 + MIT1002_02181 0.16 +1.4 2,438,347 + MIT1002_02181 0.17 +0.6 2,438,450 + MIT1002_02181 0.38 -1.0 2,438,499 - MIT1002_02181 0.49 +0.2 2,438,558 + MIT1002_02181 0.61 -1.4 2,438,590 + MIT1002_02181 0.67 -0.5 2,438,590 + MIT1002_02181 0.67 +1.5 2,438,598 - MIT1002_02181 0.69 +1.1 2,438,627 + MIT1002_02181 0.75 -1.2 2,438,913 + MIT1002_02182 0.82 -1.0 2,438,928 + -0.6 2,438,928 + +0.5 2,438,928 + +0.3 2,438,933 - +0.2 2,439,161 + MIT1002_02183 0.20 -1.6 2,439,195 + MIT1002_02183 0.23 +0.7 2,439,204 + MIT1002_02183 0.24 -0.6 2,439,204 + MIT1002_02183 0.24 -0.8 2,439,204 + MIT1002_02183 0.24 -1.1 2,439,211 - MIT1002_02183 0.24 -0.8 2,439,212 - MIT1002_02183 0.25 -2.1 2,439,212 - MIT1002_02183 0.25 +0.4 2,439,212 - MIT1002_02183 0.25 -2.0 2,439,212 - MIT1002_02183 0.25 -2.1 2,439,212 - MIT1002_02183 0.25 -1.5 2,439,239 - MIT1002_02183 0.27 +0.1 2,439,272 - MIT1002_02183 0.30 +0.0 2,439,357 + MIT1002_02183 0.37 -0.0 2,439,385 - MIT1002_02183 0.40 -2.2 2,439,435 - MIT1002_02183 0.44 -0.0 2,439,465 + MIT1002_02183 0.47 +0.4 2,439,606 + MIT1002_02183 0.59 +1.7 2,439,619 - MIT1002_02183 0.60 -1.8 2,439,648 + MIT1002_02183 0.63 +0.2 2,439,656 - MIT1002_02183 0.64 -2.1 2,439,686 - MIT1002_02183 0.66 -0.5 2,439,703 - MIT1002_02183 0.68 +0.3 2,439,763 + MIT1002_02183 0.73 +0.1 2,439,780 + MIT1002_02183 0.75 -0.7 2,439,783 - MIT1002_02183 0.75 -0.6 2,439,788 - MIT1002_02183 0.75 +0.4 2,439,788 - MIT1002_02183 0.75 -2.0 2,439,788 - MIT1002_02183 0.75 -1.7 2,439,818 + MIT1002_02183 0.78 -0.9 2,439,958 + +0.6 2,439,958 + -0.3 2,439,989 - +0.5 2,439,994 - -1.3 2,439,994 - -1.7 2,439,996 + +0.9 2,440,038 - -1.2 2,440,039 - -0.2 2,440,059 + -0.1 2,440,151 - -1.1 2,440,181 + +0.0 2,440,228 + +0.3 2,440,228 - -2.6 2,440,423 + MIT1002_02184 0.34 -1.6 2,440,423 + MIT1002_02184 0.34 -1.3 2,440,476 + MIT1002_02184 0.42 -0.7 2,440,790 - MIT1002_02184 0.87 -0.6 2,440,849 + +0.6 2,440,880 - -0.4
Or see this region's nucleotide sequence