Experiment: monoculture; Experiment A, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01112 and MIT1002_01113 are separated by 3 nucleotides MIT1002_01113 and MIT1002_01114 are separated by 9 nucleotides
MIT1002_01112: MIT1002_01112 - Homoserine O-succinyltransferase, at 1,219,380 to 1,220,318
_01112
MIT1002_01113: MIT1002_01113 - Methionine synthase, at 1,220,322 to 1,221,389
_01113
MIT1002_01114: MIT1002_01114 - Methionine synthase, at 1,221,399 to 1,224,023
_01114
Position (kb)
1220
1221
1222 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1219.334 kb on + strand at 1219.334 kb on + strand at 1219.334 kb on + strand at 1219.334 kb on + strand at 1219.349 kb on - strand at 1219.368 kb on + strand at 1219.383 kb on - strand at 1219.384 kb on - strand at 1219.384 kb on - strand at 1219.408 kb on - strand at 1219.423 kb on - strand at 1219.449 kb on + strand at 1219.449 kb on + strand at 1219.454 kb on + strand at 1219.536 kb on + strand, within MIT1002_01112 at 1219.558 kb on - strand, within MIT1002_01112 at 1219.636 kb on - strand, within MIT1002_01112 at 1219.684 kb on + strand, within MIT1002_01112 at 1219.737 kb on - strand, within MIT1002_01112 at 1219.786 kb on + strand, within MIT1002_01112 at 1219.794 kb on - strand, within MIT1002_01112 at 1219.794 kb on - strand, within MIT1002_01112 at 1219.901 kb on - strand, within MIT1002_01112 at 1219.982 kb on + strand, within MIT1002_01112 at 1219.988 kb on - strand, within MIT1002_01112 at 1220.210 kb on - strand, within MIT1002_01112 at 1220.272 kb on - strand at 1220.286 kb on - strand at 1220.286 kb on - strand at 1220.314 kb on + strand at 1220.378 kb on - strand at 1220.401 kb on - strand at 1220.418 kb on - strand at 1220.489 kb on - strand, within MIT1002_01113 at 1220.593 kb on - strand, within MIT1002_01113 at 1220.628 kb on + strand, within MIT1002_01113 at 1220.635 kb on - strand, within MIT1002_01113 at 1220.704 kb on - strand, within MIT1002_01113 at 1220.727 kb on + strand, within MIT1002_01113 at 1220.759 kb on + strand, within MIT1002_01113 at 1220.778 kb on - strand, within MIT1002_01113 at 1220.838 kb on - strand, within MIT1002_01113 at 1220.950 kb on - strand, within MIT1002_01113 at 1221.042 kb on + strand, within MIT1002_01113 at 1221.090 kb on + strand, within MIT1002_01113 at 1221.090 kb on + strand, within MIT1002_01113 at 1221.152 kb on + strand, within MIT1002_01113 at 1221.152 kb on + strand, within MIT1002_01113 at 1221.160 kb on - strand, within MIT1002_01113 at 1221.160 kb on - strand, within MIT1002_01113 at 1221.160 kb on - strand, within MIT1002_01113 at 1221.230 kb on + strand, within MIT1002_01113 at 1221.236 kb on + strand, within MIT1002_01113 at 1221.244 kb on - strand, within MIT1002_01113 at 1221.244 kb on - strand, within MIT1002_01113 at 1221.277 kb on + strand, within MIT1002_01113 at 1221.324 kb on - strand at 1221.367 kb on + strand at 1221.384 kb on + strand at 1221.392 kb on - strand at 1221.525 kb on + strand at 1221.535 kb on + strand at 1221.577 kb on + strand at 1221.577 kb on + strand at 1221.585 kb on - strand at 1221.585 kb on - strand at 1221.588 kb on + strand at 1221.624 kb on - strand at 1221.630 kb on - strand at 1221.684 kb on + strand, within MIT1002_01114 at 1221.684 kb on + strand, within MIT1002_01114 at 1221.685 kb on + strand, within MIT1002_01114 at 1221.686 kb on + strand, within MIT1002_01114 at 1221.692 kb on - strand, within MIT1002_01114 at 1221.814 kb on + strand, within MIT1002_01114 at 1221.824 kb on + strand, within MIT1002_01114 at 1221.865 kb on + strand, within MIT1002_01114 at 1221.868 kb on - strand, within MIT1002_01114 at 1221.873 kb on - strand, within MIT1002_01114 at 1221.954 kb on - strand, within MIT1002_01114 at 1221.954 kb on - strand, within MIT1002_01114 at 1222.084 kb on + strand, within MIT1002_01114 at 1222.087 kb on - strand, within MIT1002_01114 at 1222.116 kb on + strand, within MIT1002_01114 at 1222.125 kb on + strand, within MIT1002_01114 at 1222.133 kb on - strand, within MIT1002_01114 at 1222.133 kb on - strand, within MIT1002_01114 at 1222.265 kb on + strand, within MIT1002_01114 at 1222.307 kb on - strand, within MIT1002_01114
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 6 remove 1,219,334 + -2.1 1,219,334 + -2.8 1,219,334 + -2.0 1,219,334 + -1.4 1,219,349 - -2.3 1,219,368 + -4.4 1,219,383 - -1.5 1,219,384 - -2.9 1,219,384 - -2.0 1,219,408 - -1.4 1,219,423 - -1.4 1,219,449 + -4.2 1,219,449 + -3.5 1,219,454 + +0.9 1,219,536 + MIT1002_01112 0.17 -0.7 1,219,558 - MIT1002_01112 0.19 -1.1 1,219,636 - MIT1002_01112 0.27 +0.9 1,219,684 + MIT1002_01112 0.32 -1.5 1,219,737 - MIT1002_01112 0.38 -2.3 1,219,786 + MIT1002_01112 0.43 -5.5 1,219,794 - MIT1002_01112 0.44 -2.4 1,219,794 - MIT1002_01112 0.44 -3.6 1,219,901 - MIT1002_01112 0.55 -2.1 1,219,982 + MIT1002_01112 0.64 -5.5 1,219,988 - MIT1002_01112 0.65 -1.7 1,220,210 - MIT1002_01112 0.88 -4.4 1,220,272 - +0.9 1,220,286 - -0.5 1,220,286 - -1.6 1,220,314 + +0.6 1,220,378 - -1.3 1,220,401 - -0.7 1,220,418 - -0.4 1,220,489 - MIT1002_01113 0.16 -0.2 1,220,593 - MIT1002_01113 0.25 +0.1 1,220,628 + MIT1002_01113 0.29 -0.7 1,220,635 - MIT1002_01113 0.29 -0.8 1,220,704 - MIT1002_01113 0.36 -1.1 1,220,727 + MIT1002_01113 0.38 -1.3 1,220,759 + MIT1002_01113 0.41 +2.3 1,220,778 - MIT1002_01113 0.43 +0.4 1,220,838 - MIT1002_01113 0.48 -1.1 1,220,950 - MIT1002_01113 0.59 -1.1 1,221,042 + MIT1002_01113 0.67 -0.2 1,221,090 + MIT1002_01113 0.72 -0.3 1,221,090 + MIT1002_01113 0.72 +0.3 1,221,152 + MIT1002_01113 0.78 -0.9 1,221,152 + MIT1002_01113 0.78 -1.1 1,221,160 - MIT1002_01113 0.78 -0.4 1,221,160 - MIT1002_01113 0.78 -1.9 1,221,160 - MIT1002_01113 0.78 -0.8 1,221,230 + MIT1002_01113 0.85 +0.5 1,221,236 + MIT1002_01113 0.86 -0.2 1,221,244 - MIT1002_01113 0.86 -0.7 1,221,244 - MIT1002_01113 0.86 -2.4 1,221,277 + MIT1002_01113 0.89 -1.1 1,221,324 - +0.9 1,221,367 + -0.6 1,221,384 + -0.1 1,221,392 - +0.9 1,221,525 + +0.5 1,221,535 + -0.8 1,221,577 + +0.3 1,221,577 + -1.1 1,221,585 - -0.1 1,221,585 - +0.7 1,221,588 + +0.2 1,221,624 - -0.5 1,221,630 - +1.5 1,221,684 + MIT1002_01114 0.11 +1.3 1,221,684 + MIT1002_01114 0.11 -1.0 1,221,685 + MIT1002_01114 0.11 -1.4 1,221,686 + MIT1002_01114 0.11 +0.1 1,221,692 - MIT1002_01114 0.11 +0.2 1,221,814 + MIT1002_01114 0.16 +0.6 1,221,824 + MIT1002_01114 0.16 -2.5 1,221,865 + MIT1002_01114 0.18 +1.0 1,221,868 - MIT1002_01114 0.18 +0.4 1,221,873 - MIT1002_01114 0.18 +1.5 1,221,954 - MIT1002_01114 0.21 -0.4 1,221,954 - MIT1002_01114 0.21 +1.2 1,222,084 + MIT1002_01114 0.26 -0.7 1,222,087 - MIT1002_01114 0.26 -0.8 1,222,116 + MIT1002_01114 0.27 -0.6 1,222,125 + MIT1002_01114 0.28 -0.3 1,222,133 - MIT1002_01114 0.28 -0.2 1,222,133 - MIT1002_01114 0.28 -0.5 1,222,265 + MIT1002_01114 0.33 -0.2 1,222,307 - MIT1002_01114 0.35 -0.9
Or see this region's nucleotide sequence