Experiment: monoculture; Experiment A, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01024 and MIT1002_01025 are separated by 101 nucleotides MIT1002_01025 and MIT1002_01026 are separated by 267 nucleotides MIT1002_01026 and MIT1002_01027 are separated by 176 nucleotides
MIT1002_01024: MIT1002_01024 - tRNA-specific adenosine deaminase, at 1,135,238 to 1,135,861
_01024
MIT1002_01025: MIT1002_01025 - hypothetical protein, at 1,135,963 to 1,136,118
_01025
MIT1002_01026: MIT1002_01026 - Membrane-bound lytic murein transglycosylase F precursor, at 1,136,386 to 1,137,789
_01026
MIT1002_01027: MIT1002_01027 - Phosphoribosylformylglycinamidine synthase, at 1,137,966 to 1,141,853
_01027
Position (kb)
1136
1137
1138 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1135.843 kb on - strand at 1135.883 kb on + strand at 1135.894 kb on - strand at 1135.896 kb on + strand at 1135.943 kb on + strand at 1135.943 kb on + strand at 1135.951 kb on - strand at 1135.971 kb on + strand at 1135.976 kb on + strand at 1135.978 kb on + strand at 1135.978 kb on + strand at 1136.066 kb on - strand, within MIT1002_01025 at 1136.066 kb on - strand, within MIT1002_01025 at 1136.204 kb on + strand at 1136.305 kb on - strand at 1136.331 kb on + strand at 1136.356 kb on + strand at 1136.411 kb on - strand at 1136.411 kb on - strand at 1136.452 kb on + strand at 1136.460 kb on - strand at 1136.460 kb on - strand at 1136.484 kb on + strand at 1136.519 kb on - strand at 1136.551 kb on - strand, within MIT1002_01026 at 1136.558 kb on - strand, within MIT1002_01026 at 1136.584 kb on - strand, within MIT1002_01026 at 1136.666 kb on + strand, within MIT1002_01026 at 1136.674 kb on - strand, within MIT1002_01026 at 1136.718 kb on + strand, within MIT1002_01026 at 1136.726 kb on - strand, within MIT1002_01026 at 1136.775 kb on + strand, within MIT1002_01026 at 1136.797 kb on + strand, within MIT1002_01026 at 1136.851 kb on - strand, within MIT1002_01026 at 1136.876 kb on - strand, within MIT1002_01026 at 1136.878 kb on - strand, within MIT1002_01026 at 1136.991 kb on + strand, within MIT1002_01026 at 1136.999 kb on - strand, within MIT1002_01026 at 1137.025 kb on - strand, within MIT1002_01026 at 1137.036 kb on + strand, within MIT1002_01026 at 1137.074 kb on + strand, within MIT1002_01026 at 1137.082 kb on - strand, within MIT1002_01026 at 1137.126 kb on + strand, within MIT1002_01026 at 1137.152 kb on - strand, within MIT1002_01026 at 1137.168 kb on + strand, within MIT1002_01026 at 1137.177 kb on - strand, within MIT1002_01026 at 1137.357 kb on + strand, within MIT1002_01026 at 1137.422 kb on - strand, within MIT1002_01026 at 1137.522 kb on + strand, within MIT1002_01026 at 1137.522 kb on + strand, within MIT1002_01026 at 1137.565 kb on - strand, within MIT1002_01026 at 1137.648 kb on + strand, within MIT1002_01026 at 1137.658 kb on - strand at 1137.711 kb on + strand at 1137.718 kb on + strand at 1137.786 kb on + strand at 1137.786 kb on + strand at 1137.799 kb on - strand at 1137.862 kb on - strand at 1137.864 kb on - strand at 1137.879 kb on - strand at 1137.921 kb on - strand at 1137.975 kb on - strand at 1137.999 kb on - strand at 1138.141 kb on - strand at 1138.161 kb on + strand at 1138.198 kb on - strand at 1138.226 kb on - strand at 1138.233 kb on - strand at 1138.281 kb on + strand at 1138.370 kb on + strand, within MIT1002_01027 at 1138.380 kb on + strand, within MIT1002_01027 at 1138.444 kb on + strand, within MIT1002_01027 at 1138.452 kb on - strand, within MIT1002_01027 at 1138.541 kb on - strand, within MIT1002_01027 at 1138.603 kb on + strand, within MIT1002_01027 at 1138.603 kb on + strand, within MIT1002_01027 at 1138.638 kb on + strand, within MIT1002_01027
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 6 remove 1,135,843 - -2.4 1,135,883 + -0.5 1,135,894 - +0.4 1,135,896 + -0.1 1,135,943 + -1.1 1,135,943 + +0.4 1,135,951 - +1.0 1,135,971 + -0.1 1,135,976 + +1.3 1,135,978 + +0.9 1,135,978 + -0.8 1,136,066 - MIT1002_01025 0.66 +0.9 1,136,066 - MIT1002_01025 0.66 +1.0 1,136,204 + +0.1 1,136,305 - +0.0 1,136,331 + +0.4 1,136,356 + +1.0 1,136,411 - -1.0 1,136,411 - +0.6 1,136,452 + -0.3 1,136,460 - -0.6 1,136,460 - +0.0 1,136,484 + -1.5 1,136,519 - -0.3 1,136,551 - MIT1002_01026 0.12 -0.1 1,136,558 - MIT1002_01026 0.12 +1.6 1,136,584 - MIT1002_01026 0.14 -0.6 1,136,666 + MIT1002_01026 0.20 +0.9 1,136,674 - MIT1002_01026 0.21 +0.4 1,136,718 + MIT1002_01026 0.24 -0.2 1,136,726 - MIT1002_01026 0.24 +0.7 1,136,775 + MIT1002_01026 0.28 -0.8 1,136,797 + MIT1002_01026 0.29 +0.9 1,136,851 - MIT1002_01026 0.33 -0.0 1,136,876 - MIT1002_01026 0.35 -0.7 1,136,878 - MIT1002_01026 0.35 -0.3 1,136,991 + MIT1002_01026 0.43 -2.1 1,136,999 - MIT1002_01026 0.44 +0.1 1,137,025 - MIT1002_01026 0.46 -0.1 1,137,036 + MIT1002_01026 0.46 -0.6 1,137,074 + MIT1002_01026 0.49 -0.3 1,137,082 - MIT1002_01026 0.50 -0.7 1,137,126 + MIT1002_01026 0.53 +0.0 1,137,152 - MIT1002_01026 0.55 -0.7 1,137,168 + MIT1002_01026 0.56 -2.2 1,137,177 - MIT1002_01026 0.56 +0.6 1,137,357 + MIT1002_01026 0.69 -2.3 1,137,422 - MIT1002_01026 0.74 -0.1 1,137,522 + MIT1002_01026 0.81 +0.4 1,137,522 + MIT1002_01026 0.81 +2.1 1,137,565 - MIT1002_01026 0.84 -0.2 1,137,648 + MIT1002_01026 0.90 -0.7 1,137,658 - -0.4 1,137,711 + +0.2 1,137,718 + +1.5 1,137,786 + +0.3 1,137,786 + +0.6 1,137,799 - -1.6 1,137,862 - -2.7 1,137,864 - -1.3 1,137,879 - +0.2 1,137,921 - -2.0 1,137,975 - -2.3 1,137,999 - -2.1 1,138,141 - +0.9 1,138,161 + -1.4 1,138,198 - -4.8 1,138,226 - -0.9 1,138,233 - -1.9 1,138,281 + -1.4 1,138,370 + MIT1002_01027 0.10 -0.5 1,138,380 + MIT1002_01027 0.11 -2.9 1,138,444 + MIT1002_01027 0.12 -3.5 1,138,452 - MIT1002_01027 0.12 -0.4 1,138,541 - MIT1002_01027 0.15 -2.7 1,138,603 + MIT1002_01027 0.16 +0.2 1,138,603 + MIT1002_01027 0.16 -2.0 1,138,638 + MIT1002_01027 0.17 -4.1
Or see this region's nucleotide sequence