Experiment: monoculture; Experiment A, time point 6
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00274 and MIT1002_00275 are separated by 17 nucleotides MIT1002_00275 and MIT1002_00276 are separated by 18 nucleotides MIT1002_00276 and MIT1002_00277 overlap by 1 nucleotides MIT1002_00277 and MIT1002_00278 overlap by 8 nucleotides MIT1002_00278 and MIT1002_00279 overlap by 11 nucleotides
MIT1002_00274: MIT1002_00274 - Uptake hydrogenase small subunit precursor, at 299,345 to 300,343
_00274
MIT1002_00275: MIT1002_00275 - Hydrogenase-1 large chain, at 300,361 to 302,238
_00275
MIT1002_00276: MIT1002_00276 - hypothetical protein, at 302,257 to 302,472
_00276
MIT1002_00277: MIT1002_00277 - Hydrogenase isoenzymes formation protein HypC, at 302,472 to 302,699
_00277
MIT1002_00278: MIT1002_00278 - hydrogenase nickel incorporation protein, at 302,692 to 303,078
_00278
MIT1002_00279: MIT1002_00279 - Hydrogenase isoenzymes nickel incorporation protein HypB, at 303,068 to 303,814
_00279
Position (kb)
300
301
302
303 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 299.482 kb on - strand, within MIT1002_00274 at 299.521 kb on + strand, within MIT1002_00274 at 299.531 kb on + strand, within MIT1002_00274 at 299.531 kb on + strand, within MIT1002_00274 at 299.531 kb on + strand, within MIT1002_00274 at 299.534 kb on - strand, within MIT1002_00274 at 299.554 kb on - strand, within MIT1002_00274 at 299.564 kb on - strand, within MIT1002_00274 at 299.658 kb on - strand, within MIT1002_00274 at 299.658 kb on - strand, within MIT1002_00274 at 299.724 kb on + strand, within MIT1002_00274 at 299.724 kb on + strand, within MIT1002_00274 at 299.727 kb on - strand, within MIT1002_00274 at 299.741 kb on - strand, within MIT1002_00274 at 299.809 kb on + strand, within MIT1002_00274 at 299.844 kb on - strand, within MIT1002_00274 at 299.917 kb on - strand, within MIT1002_00274 at 299.927 kb on - strand, within MIT1002_00274 at 300.034 kb on + strand, within MIT1002_00274 at 300.065 kb on - strand, within MIT1002_00274 at 300.198 kb on - strand, within MIT1002_00274 at 300.269 kb on + strand at 300.269 kb on + strand at 300.284 kb on - strand at 300.295 kb on + strand at 300.312 kb on - strand at 300.436 kb on + strand at 300.453 kb on - strand at 300.481 kb on - strand at 300.515 kb on + strand at 300.523 kb on - strand at 300.523 kb on - strand at 300.523 kb on - strand at 300.573 kb on + strand, within MIT1002_00275 at 300.573 kb on + strand, within MIT1002_00275 at 300.581 kb on + strand, within MIT1002_00275 at 300.609 kb on - strand, within MIT1002_00275 at 300.622 kb on + strand, within MIT1002_00275 at 300.664 kb on + strand, within MIT1002_00275 at 300.765 kb on - strand, within MIT1002_00275 at 300.776 kb on + strand, within MIT1002_00275 at 300.784 kb on - strand, within MIT1002_00275 at 300.817 kb on + strand, within MIT1002_00275 at 300.862 kb on - strand, within MIT1002_00275 at 300.897 kb on + strand, within MIT1002_00275 at 300.905 kb on + strand, within MIT1002_00275 at 300.990 kb on + strand, within MIT1002_00275 at 300.995 kb on + strand, within MIT1002_00275 at 300.995 kb on + strand, within MIT1002_00275 at 301.040 kb on - strand, within MIT1002_00275 at 301.088 kb on + strand, within MIT1002_00275 at 301.096 kb on - strand, within MIT1002_00275 at 301.109 kb on - strand, within MIT1002_00275 at 301.168 kb on + strand, within MIT1002_00275 at 301.216 kb on + strand, within MIT1002_00275 at 301.232 kb on - strand, within MIT1002_00275 at 301.292 kb on - strand, within MIT1002_00275 at 301.324 kb on + strand, within MIT1002_00275 at 301.340 kb on + strand, within MIT1002_00275 at 301.340 kb on + strand, within MIT1002_00275 at 301.340 kb on + strand, within MIT1002_00275 at 301.345 kb on + strand, within MIT1002_00275 at 301.348 kb on - strand, within MIT1002_00275 at 301.348 kb on - strand, within MIT1002_00275 at 301.348 kb on - strand, within MIT1002_00275 at 301.424 kb on + strand, within MIT1002_00275 at 301.424 kb on + strand, within MIT1002_00275 at 301.424 kb on + strand, within MIT1002_00275 at 301.424 kb on + strand, within MIT1002_00275 at 301.432 kb on - strand, within MIT1002_00275 at 301.432 kb on - strand, within MIT1002_00275 at 301.432 kb on - strand, within MIT1002_00275 at 301.432 kb on - strand, within MIT1002_00275 at 301.435 kb on + strand, within MIT1002_00275 at 301.436 kb on + strand, within MIT1002_00275 at 301.455 kb on + strand, within MIT1002_00275 at 301.460 kb on + strand, within MIT1002_00275 at 301.468 kb on - strand, within MIT1002_00275 at 301.468 kb on - strand, within MIT1002_00275 at 301.518 kb on + strand, within MIT1002_00275 at 301.541 kb on + strand, within MIT1002_00275 at 301.558 kb on - strand, within MIT1002_00275 at 301.591 kb on - strand, within MIT1002_00275 at 301.605 kb on + strand, within MIT1002_00275 at 301.618 kb on + strand, within MIT1002_00275 at 301.728 kb on - strand, within MIT1002_00275 at 301.728 kb on - strand, within MIT1002_00275 at 301.728 kb on - strand, within MIT1002_00275 at 301.728 kb on - strand, within MIT1002_00275 at 301.800 kb on + strand, within MIT1002_00275 at 301.842 kb on + strand, within MIT1002_00275 at 301.850 kb on - strand, within MIT1002_00275 at 301.850 kb on - strand, within MIT1002_00275 at 301.893 kb on + strand, within MIT1002_00275 at 301.893 kb on + strand, within MIT1002_00275 at 301.901 kb on - strand, within MIT1002_00275 at 301.901 kb on - strand, within MIT1002_00275 at 301.907 kb on + strand, within MIT1002_00275 at 301.943 kb on + strand, within MIT1002_00275 at 301.951 kb on - strand, within MIT1002_00275 at 302.103 kb on - strand at 302.159 kb on + strand at 302.166 kb on + strand at 302.171 kb on + strand at 302.182 kb on + strand at 302.513 kb on + strand, within MIT1002_00277 at 302.539 kb on - strand, within MIT1002_00277 at 302.575 kb on + strand, within MIT1002_00277 at 302.583 kb on - strand, within MIT1002_00277 at 302.659 kb on + strand, within MIT1002_00277 at 302.667 kb on - strand, within MIT1002_00277 at 302.678 kb on + strand at 302.678 kb on + strand at 302.686 kb on - strand at 302.737 kb on + strand, within MIT1002_00278 at 302.743 kb on - strand, within MIT1002_00278 at 302.779 kb on - strand, within MIT1002_00278 at 302.846 kb on - strand, within MIT1002_00278 at 302.882 kb on - strand, within MIT1002_00278 at 302.996 kb on - strand, within MIT1002_00278 at 303.027 kb on + strand, within MIT1002_00278 at 303.050 kb on + strand at 303.058 kb on - strand at 303.135 kb on + strand at 303.180 kb on - strand, within MIT1002_00279 at 303.213 kb on + strand, within MIT1002_00279
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 6 remove 299,482 - MIT1002_00274 0.14 +0.0 299,521 + MIT1002_00274 0.18 -0.7 299,531 + MIT1002_00274 0.19 +0.8 299,531 + MIT1002_00274 0.19 +1.1 299,531 + MIT1002_00274 0.19 -0.5 299,534 - MIT1002_00274 0.19 +0.0 299,554 - MIT1002_00274 0.21 -1.5 299,564 - MIT1002_00274 0.22 +0.4 299,658 - MIT1002_00274 0.31 -1.6 299,658 - MIT1002_00274 0.31 -0.3 299,724 + MIT1002_00274 0.38 -0.7 299,724 + MIT1002_00274 0.38 -1.4 299,727 - MIT1002_00274 0.38 +0.2 299,741 - MIT1002_00274 0.40 +1.5 299,809 + MIT1002_00274 0.46 -1.1 299,844 - MIT1002_00274 0.50 +0.0 299,917 - MIT1002_00274 0.57 -1.2 299,927 - MIT1002_00274 0.58 -3.0 300,034 + MIT1002_00274 0.69 -2.0 300,065 - MIT1002_00274 0.72 +0.3 300,198 - MIT1002_00274 0.85 +0.6 300,269 + -0.6 300,269 + -0.7 300,284 - +0.6 300,295 + -0.2 300,312 - -0.2 300,436 + +0.1 300,453 - +2.9 300,481 - +0.1 300,515 + -0.7 300,523 - +1.4 300,523 - +0.8 300,523 - +0.2 300,573 + MIT1002_00275 0.11 +2.0 300,573 + MIT1002_00275 0.11 -1.0 300,581 + MIT1002_00275 0.12 -0.4 300,609 - MIT1002_00275 0.13 -0.4 300,622 + MIT1002_00275 0.14 +1.4 300,664 + MIT1002_00275 0.16 +2.0 300,765 - MIT1002_00275 0.22 +0.8 300,776 + MIT1002_00275 0.22 +0.9 300,784 - MIT1002_00275 0.23 +0.5 300,817 + MIT1002_00275 0.24 -0.2 300,862 - MIT1002_00275 0.27 -2.7 300,897 + MIT1002_00275 0.29 -1.1 300,905 + MIT1002_00275 0.29 +0.5 300,990 + MIT1002_00275 0.33 -1.6 300,995 + MIT1002_00275 0.34 -0.6 300,995 + MIT1002_00275 0.34 -0.6 301,040 - MIT1002_00275 0.36 +1.8 301,088 + MIT1002_00275 0.39 +1.8 301,096 - MIT1002_00275 0.39 +2.8 301,109 - MIT1002_00275 0.40 -1.2 301,168 + MIT1002_00275 0.43 +0.9 301,216 + MIT1002_00275 0.46 -1.8 301,232 - MIT1002_00275 0.46 -0.6 301,292 - MIT1002_00275 0.50 -2.0 301,324 + MIT1002_00275 0.51 -1.0 301,340 + MIT1002_00275 0.52 +1.9 301,340 + MIT1002_00275 0.52 +0.8 301,340 + MIT1002_00275 0.52 -0.3 301,345 + MIT1002_00275 0.52 +0.9 301,348 - MIT1002_00275 0.53 -2.8 301,348 - MIT1002_00275 0.53 -2.0 301,348 - MIT1002_00275 0.53 -2.1 301,424 + MIT1002_00275 0.57 -2.4 301,424 + MIT1002_00275 0.57 +0.8 301,424 + MIT1002_00275 0.57 -0.2 301,424 + MIT1002_00275 0.57 +0.9 301,432 - MIT1002_00275 0.57 +0.5 301,432 - MIT1002_00275 0.57 +0.5 301,432 - MIT1002_00275 0.57 -1.0 301,432 - MIT1002_00275 0.57 +0.2 301,435 + MIT1002_00275 0.57 +1.0 301,436 + MIT1002_00275 0.57 +1.1 301,455 + MIT1002_00275 0.58 +0.6 301,460 + MIT1002_00275 0.59 +1.2 301,468 - MIT1002_00275 0.59 -1.5 301,468 - MIT1002_00275 0.59 -0.1 301,518 + MIT1002_00275 0.62 +0.6 301,541 + MIT1002_00275 0.63 -0.3 301,558 - MIT1002_00275 0.64 +0.9 301,591 - MIT1002_00275 0.65 -0.9 301,605 + MIT1002_00275 0.66 -0.5 301,618 + MIT1002_00275 0.67 -1.3 301,728 - MIT1002_00275 0.73 -0.1 301,728 - MIT1002_00275 0.73 +0.3 301,728 - MIT1002_00275 0.73 +0.8 301,728 - MIT1002_00275 0.73 +1.3 301,800 + MIT1002_00275 0.77 -0.3 301,842 + MIT1002_00275 0.79 +0.6 301,850 - MIT1002_00275 0.79 -1.2 301,850 - MIT1002_00275 0.79 +2.2 301,893 + MIT1002_00275 0.82 +0.1 301,893 + MIT1002_00275 0.82 +2.3 301,901 - MIT1002_00275 0.82 -0.5 301,901 - MIT1002_00275 0.82 -0.4 301,907 + MIT1002_00275 0.82 +0.4 301,943 + MIT1002_00275 0.84 +2.2 301,951 - MIT1002_00275 0.85 +0.7 302,103 - -0.7 302,159 + +1.8 302,166 + +0.7 302,171 + -0.3 302,182 + +0.8 302,513 + MIT1002_00277 0.18 -1.0 302,539 - MIT1002_00277 0.29 +0.2 302,575 + MIT1002_00277 0.45 -0.9 302,583 - MIT1002_00277 0.49 +0.4 302,659 + MIT1002_00277 0.82 +0.0 302,667 - MIT1002_00277 0.86 -0.2 302,678 + +0.4 302,678 + -0.9 302,686 - -0.2 302,737 + MIT1002_00278 0.12 +1.4 302,743 - MIT1002_00278 0.13 -0.6 302,779 - MIT1002_00278 0.22 -0.5 302,846 - MIT1002_00278 0.40 -2.2 302,882 - MIT1002_00278 0.49 -0.5 302,996 - MIT1002_00278 0.79 -2.2 303,027 + MIT1002_00278 0.87 -1.1 303,050 + -2.4 303,058 - -0.7 303,135 + +0.6 303,180 - MIT1002_00279 0.15 -0.3 303,213 + MIT1002_00279 0.19 +0.6
Or see this region's nucleotide sequence