Experiment: monoculture; Experiment A, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_04148 and MIT1002_04149 are separated by 703 nucleotides MIT1002_04149 and MIT1002_04150 are separated by 8 nucleotides MIT1002_04150 and MIT1002_04151 are separated by 38 nucleotides
MIT1002_04148: MIT1002_04148 - Transposase, TnpA family, at 11,665 to 14,403
_04148
MIT1002_04149: MIT1002_04149 - putative membrane protein, at 15,107 to 15,553
_04149
MIT1002_04150: MIT1002_04150 - putative membrane protein, at 15,562 to 16,032
_04150
MIT1002_04151: MIT1002_04151 - Cytochrome c oxidase subunit 2 precursor, at 16,071 to 16,769
_04151
Position (kb) 15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 14.139 kb on - strand at 14.139 kb on - strand at 14.148 kb on + strand at 14.199 kb on - strand at 14.220 kb on + strand at 14.220 kb on + strand at 14.228 kb on - strand at 14.239 kb on + strand at 14.241 kb on + strand at 14.252 kb on - strand at 14.252 kb on - strand at 14.293 kb on + strand at 14.307 kb on + strand at 14.308 kb on + strand at 14.308 kb on + strand at 14.316 kb on - strand at 14.385 kb on + strand at 14.393 kb on - strand at 14.496 kb on - strand at 14.548 kb on + strand at 14.548 kb on + strand at 14.593 kb on - strand at 14.637 kb on + strand at 14.642 kb on + strand at 14.642 kb on + strand at 14.642 kb on + strand at 14.642 kb on + strand at 14.642 kb on + strand at 14.642 kb on + strand at 14.650 kb on - strand at 14.650 kb on - strand at 14.786 kb on - strand at 14.795 kb on - strand at 14.887 kb on - strand at 14.887 kb on - strand at 14.899 kb on + strand at 14.931 kb on - strand at 14.973 kb on - strand at 15.070 kb on - strand at 15.130 kb on - strand at 15.140 kb on + strand at 15.140 kb on + strand at 15.140 kb on + strand at 15.148 kb on - strand at 15.148 kb on - strand at 15.148 kb on - strand at 15.161 kb on + strand, within MIT1002_04149 at 15.268 kb on + strand, within MIT1002_04149 at 15.330 kb on + strand, within MIT1002_04149 at 15.400 kb on - strand, within MIT1002_04149 at 15.454 kb on - strand, within MIT1002_04149 at 15.475 kb on + strand, within MIT1002_04149 at 15.491 kb on + strand, within MIT1002_04149 at 15.548 kb on + strand at 15.556 kb on - strand at 15.574 kb on + strand at 15.591 kb on + strand at 15.591 kb on + strand at 15.591 kb on + strand at 15.599 kb on - strand at 15.675 kb on - strand, within MIT1002_04150 at 15.701 kb on + strand, within MIT1002_04150 at 15.711 kb on - strand, within MIT1002_04150 at 15.711 kb on - strand, within MIT1002_04150 at 15.723 kb on - strand, within MIT1002_04150 at 15.726 kb on + strand, within MIT1002_04150 at 15.726 kb on + strand, within MIT1002_04150 at 15.726 kb on + strand, within MIT1002_04150 at 15.726 kb on + strand, within MIT1002_04150 at 15.726 kb on + strand, within MIT1002_04150 at 15.726 kb on + strand, within MIT1002_04150 at 15.734 kb on - strand, within MIT1002_04150 at 15.767 kb on + strand, within MIT1002_04150 at 15.772 kb on + strand, within MIT1002_04150 at 15.780 kb on - strand, within MIT1002_04150 at 15.836 kb on - strand, within MIT1002_04150 at 15.881 kb on - strand, within MIT1002_04150 at 15.881 kb on - strand, within MIT1002_04150 at 16.001 kb on - strand at 16.037 kb on - strand at 16.118 kb on - strand at 16.137 kb on - strand at 16.183 kb on + strand, within MIT1002_04151 at 16.215 kb on + strand, within MIT1002_04151 at 16.242 kb on + strand, within MIT1002_04151 at 16.242 kb on + strand, within MIT1002_04151 at 16.242 kb on + strand, within MIT1002_04151 at 16.250 kb on - strand, within MIT1002_04151 at 16.250 kb on - strand, within MIT1002_04151 at 16.250 kb on - strand, within MIT1002_04151 at 16.330 kb on + strand, within MIT1002_04151 at 16.330 kb on + strand, within MIT1002_04151 at 16.335 kb on + strand, within MIT1002_04151 at 16.338 kb on - strand, within MIT1002_04151 at 16.354 kb on + strand, within MIT1002_04151 at 16.398 kb on - strand, within MIT1002_04151 at 16.398 kb on - strand, within MIT1002_04151 at 16.453 kb on + strand, within MIT1002_04151 at 16.454 kb on - strand, within MIT1002_04151 at 16.465 kb on - strand, within MIT1002_04151 at 16.533 kb on + strand, within MIT1002_04151
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 4 remove 14,139 - +1.6 14,139 - +0.8 14,148 + +0.8 14,199 - +0.4 14,220 + +1.1 14,220 + -0.8 14,228 - +1.5 14,239 + +0.2 14,241 + +1.6 14,252 - -0.9 14,252 - +0.8 14,293 + +0.1 14,307 + -0.2 14,308 + -3.4 14,308 + +3.3 14,316 - +0.2 14,385 + +0.2 14,393 - +0.1 14,496 - +0.5 14,548 + +0.2 14,548 + +0.3 14,593 - +0.9 14,637 + +1.6 14,642 + +0.2 14,642 + +0.2 14,642 + -0.0 14,642 + +0.6 14,642 + +0.5 14,642 + +0.3 14,650 - +0.4 14,650 - -1.4 14,786 - -2.9 14,795 - -0.7 14,887 - -1.9 14,887 - -0.1 14,899 + -0.4 14,931 - -2.6 14,973 - -1.2 15,070 - -1.5 15,130 - -1.4 15,140 + -0.7 15,140 + +1.7 15,140 + -0.3 15,148 - -0.7 15,148 - +0.1 15,148 - +1.3 15,161 + MIT1002_04149 0.12 -2.1 15,268 + MIT1002_04149 0.36 -0.1 15,330 + MIT1002_04149 0.50 -1.1 15,400 - MIT1002_04149 0.66 +0.1 15,454 - MIT1002_04149 0.78 -1.4 15,475 + MIT1002_04149 0.82 -1.4 15,491 + MIT1002_04149 0.86 +0.4 15,548 + -0.2 15,556 - +0.1 15,574 + +1.6 15,591 + +0.7 15,591 + -1.2 15,591 + -0.4 15,599 - +0.2 15,675 - MIT1002_04150 0.24 +0.7 15,701 + MIT1002_04150 0.30 +0.2 15,711 - MIT1002_04150 0.32 -0.4 15,711 - MIT1002_04150 0.32 -1.5 15,723 - MIT1002_04150 0.34 +0.6 15,726 + MIT1002_04150 0.35 +0.1 15,726 + MIT1002_04150 0.35 -1.3 15,726 + MIT1002_04150 0.35 +0.0 15,726 + MIT1002_04150 0.35 -0.2 15,726 + MIT1002_04150 0.35 -0.4 15,726 + MIT1002_04150 0.35 +0.1 15,734 - MIT1002_04150 0.37 -0.6 15,767 + MIT1002_04150 0.44 +0.2 15,772 + MIT1002_04150 0.45 -2.1 15,780 - MIT1002_04150 0.46 -0.1 15,836 - MIT1002_04150 0.58 -0.9 15,881 - MIT1002_04150 0.68 -0.0 15,881 - MIT1002_04150 0.68 +0.9 16,001 - +0.7 16,037 - -0.5 16,118 - +0.2 16,137 - -0.9 16,183 + MIT1002_04151 0.16 +1.2 16,215 + MIT1002_04151 0.21 +0.2 16,242 + MIT1002_04151 0.24 -1.5 16,242 + MIT1002_04151 0.24 +0.6 16,242 + MIT1002_04151 0.24 -0.5 16,250 - MIT1002_04151 0.26 +1.3 16,250 - MIT1002_04151 0.26 +1.5 16,250 - MIT1002_04151 0.26 +0.2 16,330 + MIT1002_04151 0.37 +2.0 16,330 + MIT1002_04151 0.37 +0.3 16,335 + MIT1002_04151 0.38 +0.0 16,338 - MIT1002_04151 0.38 -1.4 16,354 + MIT1002_04151 0.40 +0.3 16,398 - MIT1002_04151 0.47 -0.4 16,398 - MIT1002_04151 0.47 -0.8 16,453 + MIT1002_04151 0.55 -0.6 16,454 - MIT1002_04151 0.55 -3.0 16,465 - MIT1002_04151 0.56 -0.5 16,533 + MIT1002_04151 0.66 +0.9
Or see this region's nucleotide sequence