Experiment: monoculture; Experiment A, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03331 and MIT1002_03332 are separated by 274 nucleotides MIT1002_03332 and MIT1002_03333 are separated by 35 nucleotides MIT1002_03333 and MIT1002_03334 are separated by 8 nucleotides MIT1002_03334 and MIT1002_03335 overlap by 7 nucleotides MIT1002_03335 and MIT1002_03336 are separated by 54 nucleotides
MIT1002_03331: MIT1002_03331 - D-lactate dehydrogenase, at 3,721,763 to 3,723,496
_03331
MIT1002_03332: MIT1002_03332 - CsgBAC operon transcriptional regulatory protein, at 3,723,771 to 3,724,448
_03332
MIT1002_03333: MIT1002_03333 - curli production assembly/transport protein CsgG, at 3,724,484 to 3,725,245
_03333
MIT1002_03334: MIT1002_03334 - curli assembly protein CsgF, at 3,725,254 to 3,725,709
_03334
MIT1002_03335: MIT1002_03335 - curli assembly protein CsgE, at 3,725,703 to 3,726,095
_03335
MIT1002_03336: MIT1002_03336 - curlin minor subunit CsgB, at 3,726,150 to 3,727,595
_03336
Position (kb)
3724
3725
3726 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9 at 3723.485 kb on - strand at 3723.492 kb on - strand at 3723.537 kb on + strand at 3723.545 kb on - strand at 3723.545 kb on - strand at 3723.625 kb on + strand at 3723.700 kb on + strand at 3723.703 kb on - strand at 3723.703 kb on - strand at 3723.708 kb on - strand at 3723.708 kb on - strand at 3723.710 kb on + strand at 3723.817 kb on + strand at 3723.835 kb on - strand at 3723.904 kb on + strand, within MIT1002_03332 at 3723.925 kb on + strand, within MIT1002_03332 at 3723.931 kb on - strand, within MIT1002_03332 at 3723.931 kb on - strand, within MIT1002_03332 at 3723.933 kb on - strand, within MIT1002_03332 at 3723.958 kb on - strand, within MIT1002_03332 at 3723.959 kb on - strand, within MIT1002_03332 at 3724.007 kb on + strand, within MIT1002_03332 at 3724.034 kb on - strand, within MIT1002_03332 at 3724.059 kb on + strand, within MIT1002_03332 at 3724.081 kb on + strand, within MIT1002_03332 at 3724.110 kb on + strand, within MIT1002_03332 at 3724.142 kb on - strand, within MIT1002_03332 at 3724.199 kb on + strand, within MIT1002_03332 at 3724.232 kb on + strand, within MIT1002_03332 at 3724.250 kb on - strand, within MIT1002_03332 at 3724.255 kb on - strand, within MIT1002_03332 at 3724.315 kb on + strand, within MIT1002_03332 at 3724.396 kb on - strand at 3724.450 kb on - strand at 3724.507 kb on - strand at 3724.510 kb on + strand at 3724.632 kb on + strand, within MIT1002_03333 at 3724.642 kb on + strand, within MIT1002_03333 at 3724.647 kb on + strand, within MIT1002_03333 at 3724.650 kb on - strand, within MIT1002_03333 at 3724.650 kb on - strand, within MIT1002_03333 at 3724.655 kb on - strand, within MIT1002_03333 at 3724.661 kb on + strand, within MIT1002_03333 at 3724.662 kb on - strand, within MIT1002_03333 at 3724.669 kb on - strand, within MIT1002_03333 at 3724.669 kb on - strand, within MIT1002_03333 at 3724.697 kb on + strand, within MIT1002_03333 at 3724.697 kb on + strand, within MIT1002_03333 at 3724.707 kb on + strand, within MIT1002_03333 at 3724.712 kb on - strand, within MIT1002_03333 at 3724.714 kb on + strand, within MIT1002_03333 at 3724.749 kb on + strand, within MIT1002_03333 at 3724.749 kb on + strand, within MIT1002_03333 at 3724.757 kb on - strand, within MIT1002_03333 at 3724.757 kb on - strand, within MIT1002_03333 at 3724.783 kb on + strand, within MIT1002_03333 at 3724.784 kb on + strand, within MIT1002_03333 at 3724.792 kb on - strand, within MIT1002_03333 at 3724.857 kb on + strand, within MIT1002_03333 at 3724.943 kb on - strand, within MIT1002_03333 at 3724.971 kb on + strand, within MIT1002_03333 at 3724.977 kb on - strand, within MIT1002_03333 at 3725.003 kb on - strand, within MIT1002_03333 at 3725.043 kb on + strand, within MIT1002_03333 at 3725.051 kb on - strand, within MIT1002_03333 at 3725.051 kb on - strand, within MIT1002_03333 at 3725.053 kb on + strand, within MIT1002_03333 at 3725.074 kb on + strand, within MIT1002_03333 at 3725.122 kb on + strand, within MIT1002_03333 at 3725.179 kb on + strand at 3725.240 kb on + strand at 3725.279 kb on + strand at 3725.310 kb on - strand, within MIT1002_03334 at 3725.319 kb on - strand, within MIT1002_03334 at 3725.338 kb on - strand, within MIT1002_03334 at 3725.370 kb on + strand, within MIT1002_03334 at 3725.494 kb on + strand, within MIT1002_03334 at 3725.503 kb on + strand, within MIT1002_03334 at 3725.508 kb on + strand, within MIT1002_03334 at 3725.533 kb on - strand, within MIT1002_03334 at 3725.545 kb on - strand, within MIT1002_03334 at 3725.623 kb on + strand, within MIT1002_03334 at 3725.689 kb on - strand at 3725.729 kb on + strand at 3725.739 kb on - strand at 3725.830 kb on - strand, within MIT1002_03335 at 3725.843 kb on + strand, within MIT1002_03335 at 3725.925 kb on + strand, within MIT1002_03335 at 3725.925 kb on + strand, within MIT1002_03335 at 3725.933 kb on - strand, within MIT1002_03335 at 3725.933 kb on - strand, within MIT1002_03335 at 3725.933 kb on - strand, within MIT1002_03335 at 3725.933 kb on - strand, within MIT1002_03335 at 3725.943 kb on + strand, within MIT1002_03335 at 3725.943 kb on + strand, within MIT1002_03335 at 3725.943 kb on + strand, within MIT1002_03335 at 3725.943 kb on + strand, within MIT1002_03335 at 3726.107 kb on - strand at 3726.148 kb on + strand at 3726.197 kb on + strand at 3726.199 kb on - strand at 3726.199 kb on - strand at 3726.205 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 4 remove 3,723,485 - -5.0 3,723,492 - -0.5 3,723,537 + -3.5 3,723,545 - -2.6 3,723,545 - -1.0 3,723,625 + -2.5 3,723,700 + +4.1 3,723,703 - +3.4 3,723,703 - +3.3 3,723,708 - +4.7 3,723,708 - +4.9 3,723,710 + +7.2 3,723,817 + +8.1 3,723,835 - +7.4 3,723,904 + MIT1002_03332 0.20 +7.6 3,723,925 + MIT1002_03332 0.23 +8.7 3,723,931 - MIT1002_03332 0.24 +8.4 3,723,931 - MIT1002_03332 0.24 +8.7 3,723,933 - MIT1002_03332 0.24 +9.2 3,723,958 - MIT1002_03332 0.28 +8.3 3,723,959 - MIT1002_03332 0.28 +8.2 3,724,007 + MIT1002_03332 0.35 +7.7 3,724,034 - MIT1002_03332 0.39 +8.1 3,724,059 + MIT1002_03332 0.42 +8.1 3,724,081 + MIT1002_03332 0.46 +8.0 3,724,110 + MIT1002_03332 0.50 +7.2 3,724,142 - MIT1002_03332 0.55 +9.2 3,724,199 + MIT1002_03332 0.63 +5.7 3,724,232 + MIT1002_03332 0.68 -1.0 3,724,250 - MIT1002_03332 0.71 +8.1 3,724,255 - MIT1002_03332 0.71 +7.9 3,724,315 + MIT1002_03332 0.80 +7.8 3,724,396 - +8.7 3,724,450 - +1.4 3,724,507 - +8.3 3,724,510 + +7.8 3,724,632 + MIT1002_03333 0.19 +6.6 3,724,642 + MIT1002_03333 0.21 +8.0 3,724,647 + MIT1002_03333 0.21 +7.2 3,724,650 - MIT1002_03333 0.22 +8.1 3,724,650 - MIT1002_03333 0.22 +8.4 3,724,655 - MIT1002_03333 0.22 +8.8 3,724,661 + MIT1002_03333 0.23 +8.2 3,724,662 - MIT1002_03333 0.23 +7.8 3,724,669 - MIT1002_03333 0.24 +8.7 3,724,669 - MIT1002_03333 0.24 +7.8 3,724,697 + MIT1002_03333 0.28 +6.3 3,724,697 + MIT1002_03333 0.28 +7.8 3,724,707 + MIT1002_03333 0.29 +8.0 3,724,712 - MIT1002_03333 0.30 +7.8 3,724,714 + MIT1002_03333 0.30 +7.5 3,724,749 + MIT1002_03333 0.35 +7.5 3,724,749 + MIT1002_03333 0.35 +7.7 3,724,757 - MIT1002_03333 0.36 +8.3 3,724,757 - MIT1002_03333 0.36 +7.9 3,724,783 + MIT1002_03333 0.39 +7.7 3,724,784 + MIT1002_03333 0.39 +8.8 3,724,792 - MIT1002_03333 0.40 +8.0 3,724,857 + MIT1002_03333 0.49 +8.6 3,724,943 - MIT1002_03333 0.60 +8.3 3,724,971 + MIT1002_03333 0.64 +7.8 3,724,977 - MIT1002_03333 0.65 +7.6 3,725,003 - MIT1002_03333 0.68 +8.4 3,725,043 + MIT1002_03333 0.73 +7.7 3,725,051 - MIT1002_03333 0.74 +7.8 3,725,051 - MIT1002_03333 0.74 +7.7 3,725,053 + MIT1002_03333 0.75 +7.2 3,725,074 + MIT1002_03333 0.77 +8.1 3,725,122 + MIT1002_03333 0.84 +7.6 3,725,179 + +7.9 3,725,240 + +8.2 3,725,279 + +8.6 3,725,310 - MIT1002_03334 0.12 +9.1 3,725,319 - MIT1002_03334 0.14 +7.9 3,725,338 - MIT1002_03334 0.18 +7.3 3,725,370 + MIT1002_03334 0.25 +8.3 3,725,494 + MIT1002_03334 0.53 +7.5 3,725,503 + MIT1002_03334 0.55 +9.0 3,725,508 + MIT1002_03334 0.56 +7.5 3,725,533 - MIT1002_03334 0.61 +7.8 3,725,545 - MIT1002_03334 0.64 +6.0 3,725,623 + MIT1002_03334 0.81 +8.9 3,725,689 - +7.9 3,725,729 + +8.3 3,725,739 - +1.3 3,725,830 - MIT1002_03335 0.32 +4.8 3,725,843 + MIT1002_03335 0.36 +7.1 3,725,925 + MIT1002_03335 0.56 +6.6 3,725,925 + MIT1002_03335 0.56 +8.3 3,725,933 - MIT1002_03335 0.59 +4.3 3,725,933 - MIT1002_03335 0.59 +4.6 3,725,933 - MIT1002_03335 0.59 +4.5 3,725,933 - MIT1002_03335 0.59 +5.2 3,725,943 + MIT1002_03335 0.61 +9.0 3,725,943 + MIT1002_03335 0.61 +6.7 3,725,943 + MIT1002_03335 0.61 +7.4 3,725,943 + MIT1002_03335 0.61 +6.7 3,726,107 - -0.3 3,726,148 + +9.2 3,726,197 + +8.1 3,726,199 - +8.6 3,726,199 - +7.8 3,726,205 + +8.6
Or see this region's nucleotide sequence