Experiment: monoculture; Experiment A, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01333 and MIT1002_01334 are separated by 586 nucleotides MIT1002_01334 and MIT1002_01335 overlap by 11 nucleotides MIT1002_01335 and MIT1002_01336 are separated by 29 nucleotides
MIT1002_01333: MIT1002_01333 - Holliday junction ATP-dependent DNA helicase RuvB, at 1,479,174 to 1,480,178
_01333
MIT1002_01334: MIT1002_01334 - Acyl-CoA thioester hydrolase YbgC, at 1,480,765 to 1,481,163
_01334
MIT1002_01335: MIT1002_01335 - Biopolymer transport protein ExbB, at 1,481,153 to 1,481,842
_01335
MIT1002_01336: MIT1002_01336 - Biopolymer transport protein ExbD, at 1,481,872 to 1,482,291
_01336
Position (kb)
1480
1481
1482 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1480.228 kb on - strand at 1480.315 kb on + strand at 1480.343 kb on + strand at 1480.520 kb on + strand at 1480.538 kb on - strand at 1480.538 kb on - strand at 1480.548 kb on + strand at 1480.679 kb on - strand at 1480.702 kb on - strand at 1480.856 kb on + strand, within MIT1002_01334 at 1480.862 kb on - strand, within MIT1002_01334 at 1480.883 kb on + strand, within MIT1002_01334 at 1480.891 kb on - strand, within MIT1002_01334 at 1481.059 kb on + strand, within MIT1002_01334 at 1481.095 kb on + strand, within MIT1002_01334 at 1481.837 kb on + strand at 1481.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 4 remove 1,480,228 - -0.2 1,480,315 + +0.2 1,480,343 + -1.7 1,480,520 + +0.5 1,480,538 - -0.1 1,480,538 - -0.2 1,480,548 + -0.6 1,480,679 - +0.8 1,480,702 - -1.4 1,480,856 + MIT1002_01334 0.23 -0.3 1,480,862 - MIT1002_01334 0.24 +0.5 1,480,883 + MIT1002_01334 0.30 +0.1 1,480,891 - MIT1002_01334 0.32 +4.2 1,481,059 + MIT1002_01334 0.74 +1.7 1,481,095 + MIT1002_01334 0.83 +0.3 1,481,837 + +0.7 1,481,837 + +0.7
Or see this region's nucleotide sequence