Experiment: monoculture; Experiment A, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00122 and MIT1002_00123 are separated by 256 nucleotides MIT1002_00123 and MIT1002_00124 are separated by 235 nucleotides MIT1002_00124 and MIT1002_00125 are separated by 137 nucleotides MIT1002_00125 and MIT1002_00126 are separated by 224 nucleotides
MIT1002_00122: MIT1002_00122 - UvsW helicase, at 131,341 to 133,731
_00122
MIT1002_00123: MIT1002_00123 - hypothetical protein, at 133,988 to 134,191
_00123
MIT1002_00124: MIT1002_00124 - Outer membrane protein W precursor, at 134,427 to 135,098
_00124
MIT1002_00125: MIT1002_00125 - hypothetical protein, at 135,236 to 135,703
_00125
MIT1002_00126: MIT1002_00126 - hypothetical protein, at 135,928 to 136,236
_00126
Position (kb)
134
135
136 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 133.430 kb on + strand, within MIT1002_00122 at 133.438 kb on - strand, within MIT1002_00122 at 133.491 kb on - strand, within MIT1002_00122 at 133.498 kb on - strand at 133.503 kb on - strand at 133.519 kb on + strand at 133.521 kb on - strand at 133.522 kb on + strand at 133.522 kb on + strand at 133.527 kb on - strand at 133.565 kb on + strand at 133.565 kb on + strand at 133.611 kb on + strand at 133.716 kb on - strand at 133.789 kb on - strand at 133.791 kb on + strand at 133.791 kb on + strand at 133.799 kb on - strand at 133.811 kb on + strand at 133.818 kb on - strand at 133.819 kb on - strand at 133.890 kb on - strand at 134.052 kb on - strand, within MIT1002_00123 at 134.052 kb on - strand, within MIT1002_00123 at 134.054 kb on + strand, within MIT1002_00123 at 134.054 kb on + strand, within MIT1002_00123 at 134.062 kb on + strand, within MIT1002_00123 at 134.062 kb on - strand, within MIT1002_00123 at 134.062 kb on - strand, within MIT1002_00123 at 134.069 kb on + strand, within MIT1002_00123 at 134.070 kb on - strand, within MIT1002_00123 at 134.111 kb on - strand, within MIT1002_00123 at 134.116 kb on - strand, within MIT1002_00123 at 134.116 kb on - strand, within MIT1002_00123 at 134.153 kb on - strand, within MIT1002_00123 at 134.180 kb on + strand at 134.188 kb on - strand at 134.233 kb on - strand at 134.304 kb on + strand at 134.370 kb on + strand at 134.387 kb on - strand at 134.387 kb on - strand at 134.389 kb on + strand at 134.389 kb on + strand at 134.397 kb on - strand at 134.397 kb on - strand at 134.456 kb on + strand at 134.490 kb on + strand at 134.507 kb on + strand, within MIT1002_00124 at 134.526 kb on + strand, within MIT1002_00124 at 134.587 kb on + strand, within MIT1002_00124 at 134.595 kb on - strand, within MIT1002_00124 at 134.691 kb on + strand, within MIT1002_00124 at 134.696 kb on + strand, within MIT1002_00124 at 134.702 kb on + strand, within MIT1002_00124 at 134.725 kb on - strand, within MIT1002_00124 at 134.861 kb on + strand, within MIT1002_00124 at 134.869 kb on - strand, within MIT1002_00124 at 134.874 kb on - strand, within MIT1002_00124 at 134.948 kb on - strand, within MIT1002_00124 at 135.133 kb on + strand at 135.170 kb on + strand at 135.222 kb on + strand at 135.334 kb on + strand, within MIT1002_00125 at 135.334 kb on - strand, within MIT1002_00125 at 135.432 kb on + strand, within MIT1002_00125 at 135.447 kb on - strand, within MIT1002_00125 at 135.459 kb on - strand, within MIT1002_00125 at 135.465 kb on + strand, within MIT1002_00125 at 135.473 kb on - strand, within MIT1002_00125 at 135.557 kb on - strand, within MIT1002_00125 at 135.573 kb on + strand, within MIT1002_00125 at 135.595 kb on - strand, within MIT1002_00125 at 135.597 kb on + strand, within MIT1002_00125 at 135.680 kb on + strand at 135.688 kb on - strand at 135.688 kb on - strand at 135.820 kb on + strand at 135.829 kb on - strand at 135.861 kb on - strand at 135.867 kb on - strand at 135.867 kb on - strand at 135.896 kb on + strand at 135.918 kb on + strand at 136.046 kb on + strand, within MIT1002_00126 at 136.054 kb on - strand, within MIT1002_00126 at 136.074 kb on - strand, within MIT1002_00126 at 136.092 kb on + strand, within MIT1002_00126 at 136.095 kb on - strand, within MIT1002_00126
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 4 remove 133,430 + MIT1002_00122 0.87 -0.4 133,438 - MIT1002_00122 0.88 -1.4 133,491 - MIT1002_00122 0.90 -0.6 133,498 - -1.1 133,503 - -0.2 133,519 + +0.4 133,521 - -0.9 133,522 + +0.0 133,522 + -1.0 133,527 - +0.0 133,565 + -0.6 133,565 + -0.3 133,611 + +0.5 133,716 - +0.4 133,789 - -0.2 133,791 + -0.2 133,791 + +2.3 133,799 - +1.4 133,811 + +0.6 133,818 - -1.1 133,819 - +1.8 133,890 - +0.1 134,052 - MIT1002_00123 0.31 -2.4 134,052 - MIT1002_00123 0.31 +2.1 134,054 + MIT1002_00123 0.32 -0.7 134,054 + MIT1002_00123 0.32 +0.1 134,062 + MIT1002_00123 0.36 +0.2 134,062 - MIT1002_00123 0.36 +1.3 134,062 - MIT1002_00123 0.36 -1.7 134,069 + MIT1002_00123 0.40 +1.0 134,070 - MIT1002_00123 0.40 -0.0 134,111 - MIT1002_00123 0.60 +0.5 134,116 - MIT1002_00123 0.63 -1.1 134,116 - MIT1002_00123 0.63 +1.5 134,153 - MIT1002_00123 0.81 -0.8 134,180 + +0.5 134,188 - -1.7 134,233 - -0.4 134,304 + -0.6 134,370 + -1.0 134,387 - +1.2 134,387 - +1.5 134,389 + -0.7 134,389 + +1.0 134,397 - +0.3 134,397 - +1.4 134,456 + +0.4 134,490 + +0.2 134,507 + MIT1002_00124 0.12 -0.8 134,526 + MIT1002_00124 0.15 +1.0 134,587 + MIT1002_00124 0.24 +0.5 134,595 - MIT1002_00124 0.25 -0.2 134,691 + MIT1002_00124 0.39 -0.4 134,696 + MIT1002_00124 0.40 +0.3 134,702 + MIT1002_00124 0.41 +0.6 134,725 - MIT1002_00124 0.44 -0.7 134,861 + MIT1002_00124 0.65 -0.7 134,869 - MIT1002_00124 0.66 -2.0 134,874 - MIT1002_00124 0.67 -2.3 134,948 - MIT1002_00124 0.78 -2.2 135,133 + +1.9 135,170 + -0.5 135,222 + -0.5 135,334 + MIT1002_00125 0.21 -1.1 135,334 - MIT1002_00125 0.21 -0.2 135,432 + MIT1002_00125 0.42 +0.2 135,447 - MIT1002_00125 0.45 +0.2 135,459 - MIT1002_00125 0.48 +0.9 135,465 + MIT1002_00125 0.49 +0.5 135,473 - MIT1002_00125 0.51 -0.5 135,557 - MIT1002_00125 0.69 -1.1 135,573 + MIT1002_00125 0.72 -0.3 135,595 - MIT1002_00125 0.77 -0.0 135,597 + MIT1002_00125 0.77 -0.8 135,680 + +0.8 135,688 - -1.9 135,688 - -2.3 135,820 + -0.7 135,829 - -1.5 135,861 - -3.3 135,867 - -1.1 135,867 - +1.2 135,896 + -1.2 135,918 + +0.3 136,046 + MIT1002_00126 0.38 +1.4 136,054 - MIT1002_00126 0.41 -0.3 136,074 - MIT1002_00126 0.47 -1.5 136,092 + MIT1002_00126 0.53 -0.3 136,095 - MIT1002_00126 0.54 -1.0
Or see this region's nucleotide sequence