Experiment: monoculture; Experiment A, time point 4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00044 and MIT1002_00045 are separated by 38 nucleotides MIT1002_00045 and MIT1002_00046 are separated by 218 nucleotides MIT1002_00046 and MIT1002_00047 are separated by 256 nucleotides MIT1002_00047 and MIT1002_00048 are separated by 115 nucleotides
MIT1002_00044: MIT1002_00044 - hypothetical protein, at 49,386 to 51,137
_00044
MIT1002_00045: MIT1002_00045 - hypothetical protein, at 51,176 to 51,511
_00045
MIT1002_00046: MIT1002_00046 - Bacterial leucyl aminopeptidase precursor, at 51,730 to 53,337
_00046
MIT1002_00047: MIT1002_00047 - Synthetically lethal with a defective Min system protein A, at 53,594 to 54,181
_00047
MIT1002_00048: MIT1002_00048 - DNA/pantothenate metabolism flavoprotein, at 54,297 to 55,526
_00048
Position (kb)
51
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 50.758 kb on - strand, within MIT1002_00044 at 50.766 kb on + strand, within MIT1002_00044 at 50.865 kb on - strand, within MIT1002_00044 at 50.865 kb on - strand, within MIT1002_00044 at 50.869 kb on + strand, within MIT1002_00044 at 50.902 kb on - strand, within MIT1002_00044 at 50.903 kb on - strand, within MIT1002_00044 at 50.904 kb on + strand, within MIT1002_00044 at 50.990 kb on - strand at 51.040 kb on - strand at 51.048 kb on - strand at 51.078 kb on - strand at 51.088 kb on + strand at 51.134 kb on + strand at 51.134 kb on + strand at 51.142 kb on - strand at 51.142 kb on - strand at 51.206 kb on + strand at 51.430 kb on + strand, within MIT1002_00045 at 51.430 kb on + strand, within MIT1002_00045 at 51.438 kb on - strand, within MIT1002_00045 at 51.438 kb on - strand, within MIT1002_00045 at 51.545 kb on + strand at 51.631 kb on - strand at 51.641 kb on - strand at 51.641 kb on - strand at 51.669 kb on + strand at 51.679 kb on + strand at 51.743 kb on + strand at 51.757 kb on + strand at 51.757 kb on + strand at 51.765 kb on - strand at 51.765 kb on - strand at 51.765 kb on - strand at 51.765 kb on - strand at 51.896 kb on - strand, within MIT1002_00046 at 52.048 kb on - strand, within MIT1002_00046 at 52.058 kb on - strand, within MIT1002_00046 at 52.069 kb on - strand, within MIT1002_00046 at 52.122 kb on - strand, within MIT1002_00046 at 52.225 kb on - strand, within MIT1002_00046 at 52.242 kb on - strand, within MIT1002_00046 at 52.322 kb on + strand, within MIT1002_00046 at 52.323 kb on + strand, within MIT1002_00046 at 52.328 kb on + strand, within MIT1002_00046 at 52.333 kb on + strand, within MIT1002_00046 at 52.341 kb on - strand, within MIT1002_00046 at 52.356 kb on - strand, within MIT1002_00046 at 52.376 kb on + strand, within MIT1002_00046 at 52.384 kb on - strand, within MIT1002_00046 at 52.384 kb on - strand, within MIT1002_00046 at 52.393 kb on - strand, within MIT1002_00046 at 52.393 kb on - strand, within MIT1002_00046 at 52.424 kb on - strand, within MIT1002_00046 at 52.424 kb on - strand, within MIT1002_00046 at 52.424 kb on - strand, within MIT1002_00046 at 52.450 kb on + strand, within MIT1002_00046 at 52.501 kb on - strand, within MIT1002_00046 at 52.522 kb on + strand, within MIT1002_00046 at 52.527 kb on - strand, within MIT1002_00046 at 52.527 kb on - strand, within MIT1002_00046 at 52.534 kb on + strand, within MIT1002_00046 at 52.537 kb on - strand, within MIT1002_00046 at 52.589 kb on + strand, within MIT1002_00046 at 52.624 kb on - strand, within MIT1002_00046 at 52.660 kb on + strand, within MIT1002_00046 at 52.680 kb on + strand, within MIT1002_00046 at 52.749 kb on + strand, within MIT1002_00046 at 52.770 kb on - strand, within MIT1002_00046 at 52.802 kb on - strand, within MIT1002_00046 at 52.883 kb on + strand, within MIT1002_00046 at 52.883 kb on + strand, within MIT1002_00046 at 52.883 kb on + strand, within MIT1002_00046 at 52.891 kb on - strand, within MIT1002_00046 at 52.977 kb on - strand, within MIT1002_00046 at 53.017 kb on - strand, within MIT1002_00046 at 53.021 kb on + strand, within MIT1002_00046 at 53.028 kb on + strand, within MIT1002_00046 at 53.028 kb on + strand, within MIT1002_00046 at 53.028 kb on + strand, within MIT1002_00046 at 53.028 kb on + strand, within MIT1002_00046 at 53.029 kb on - strand, within MIT1002_00046 at 53.029 kb on - strand, within MIT1002_00046 at 53.029 kb on - strand, within MIT1002_00046 at 53.036 kb on - strand, within MIT1002_00046 at 53.038 kb on + strand, within MIT1002_00046 at 53.041 kb on - strand, within MIT1002_00046 at 53.052 kb on - strand, within MIT1002_00046 at 53.065 kb on + strand, within MIT1002_00046 at 53.138 kb on - strand, within MIT1002_00046 at 53.144 kb on + strand, within MIT1002_00046 at 53.144 kb on + strand, within MIT1002_00046 at 53.152 kb on - strand, within MIT1002_00046 at 53.163 kb on + strand, within MIT1002_00046 at 53.163 kb on + strand, within MIT1002_00046 at 53.163 kb on + strand, within MIT1002_00046 at 53.171 kb on - strand, within MIT1002_00046 at 53.171 kb on - strand, within MIT1002_00046 at 53.171 kb on - strand, within MIT1002_00046 at 53.171 kb on - strand, within MIT1002_00046 at 53.171 kb on - strand, within MIT1002_00046 at 53.180 kb on + strand at 53.180 kb on + strand at 53.181 kb on + strand at 53.188 kb on - strand at 53.188 kb on - strand at 53.188 kb on - strand at 53.188 kb on - strand at 53.232 kb on + strand at 53.241 kb on - strand at 53.261 kb on + strand at 53.297 kb on - strand at 53.434 kb on + strand at 53.570 kb on + strand at 53.570 kb on - strand at 54.246 kb on - strand at 54.246 kb on - strand at 54.324 kb on - strand at 54.324 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 4 remove 50,758 - MIT1002_00044 0.78 -0.4 50,766 + MIT1002_00044 0.79 -0.8 50,865 - MIT1002_00044 0.84 +2.3 50,865 - MIT1002_00044 0.84 +0.7 50,869 + MIT1002_00044 0.85 -1.6 50,902 - MIT1002_00044 0.87 -1.6 50,903 - MIT1002_00044 0.87 +2.1 50,904 + MIT1002_00044 0.87 +1.5 50,990 - +0.2 51,040 - +1.0 51,048 - +1.4 51,078 - -0.4 51,088 + +0.9 51,134 + -0.1 51,134 + +0.9 51,142 - -0.6 51,142 - -0.5 51,206 + -0.4 51,430 + MIT1002_00045 0.76 +0.8 51,430 + MIT1002_00045 0.76 +1.0 51,438 - MIT1002_00045 0.78 -0.1 51,438 - MIT1002_00045 0.78 -1.1 51,545 + +1.5 51,631 - -2.3 51,641 - +1.4 51,641 - +1.4 51,669 + -0.7 51,679 + -1.1 51,743 + +0.6 51,757 + -0.1 51,757 + -1.9 51,765 - +0.3 51,765 - +1.3 51,765 - +1.2 51,765 - -0.0 51,896 - MIT1002_00046 0.10 -1.1 52,048 - MIT1002_00046 0.20 +0.6 52,058 - MIT1002_00046 0.20 -0.3 52,069 - MIT1002_00046 0.21 -0.5 52,122 - MIT1002_00046 0.24 -0.8 52,225 - MIT1002_00046 0.31 -1.4 52,242 - MIT1002_00046 0.32 -0.1 52,322 + MIT1002_00046 0.37 -1.1 52,323 + MIT1002_00046 0.37 -1.3 52,328 + MIT1002_00046 0.37 +1.0 52,333 + MIT1002_00046 0.38 -1.0 52,341 - MIT1002_00046 0.38 -0.3 52,356 - MIT1002_00046 0.39 +0.1 52,376 + MIT1002_00046 0.40 +0.6 52,384 - MIT1002_00046 0.41 +0.7 52,384 - MIT1002_00046 0.41 -0.7 52,393 - MIT1002_00046 0.41 -0.2 52,393 - MIT1002_00046 0.41 +0.5 52,424 - MIT1002_00046 0.43 +0.5 52,424 - MIT1002_00046 0.43 +2.5 52,424 - MIT1002_00046 0.43 +0.9 52,450 + MIT1002_00046 0.45 -1.0 52,501 - MIT1002_00046 0.48 -1.5 52,522 + MIT1002_00046 0.49 -0.3 52,527 - MIT1002_00046 0.50 +0.1 52,527 - MIT1002_00046 0.50 -0.3 52,534 + MIT1002_00046 0.50 +1.6 52,537 - MIT1002_00046 0.50 +0.7 52,589 + MIT1002_00046 0.53 +0.5 52,624 - MIT1002_00046 0.56 +0.8 52,660 + MIT1002_00046 0.58 -0.3 52,680 + MIT1002_00046 0.59 -1.0 52,749 + MIT1002_00046 0.63 -0.3 52,770 - MIT1002_00046 0.65 -0.1 52,802 - MIT1002_00046 0.67 +0.1 52,883 + MIT1002_00046 0.72 +0.7 52,883 + MIT1002_00046 0.72 -0.8 52,883 + MIT1002_00046 0.72 -0.2 52,891 - MIT1002_00046 0.72 +0.5 52,977 - MIT1002_00046 0.78 +0.7 53,017 - MIT1002_00046 0.80 +1.3 53,021 + MIT1002_00046 0.80 +1.2 53,028 + MIT1002_00046 0.81 +1.1 53,028 + MIT1002_00046 0.81 +0.9 53,028 + MIT1002_00046 0.81 +0.6 53,028 + MIT1002_00046 0.81 +2.7 53,029 - MIT1002_00046 0.81 +1.1 53,029 - MIT1002_00046 0.81 +0.5 53,029 - MIT1002_00046 0.81 -0.1 53,036 - MIT1002_00046 0.81 +0.4 53,038 + MIT1002_00046 0.81 +1.0 53,041 - MIT1002_00046 0.82 +0.7 53,052 - MIT1002_00046 0.82 +2.3 53,065 + MIT1002_00046 0.83 -0.1 53,138 - MIT1002_00046 0.88 -0.4 53,144 + MIT1002_00046 0.88 +0.0 53,144 + MIT1002_00046 0.88 +1.1 53,152 - MIT1002_00046 0.88 -0.5 53,163 + MIT1002_00046 0.89 -1.1 53,163 + MIT1002_00046 0.89 -1.1 53,163 + MIT1002_00046 0.89 +0.2 53,171 - MIT1002_00046 0.90 +0.4 53,171 - MIT1002_00046 0.90 +0.9 53,171 - MIT1002_00046 0.90 +1.0 53,171 - MIT1002_00046 0.90 -2.4 53,171 - MIT1002_00046 0.90 +1.3 53,180 + -0.8 53,180 + -1.7 53,181 + +0.0 53,188 - -0.6 53,188 - +0.5 53,188 - -1.2 53,188 - -0.1 53,232 + +0.5 53,241 - +2.4 53,261 + +0.1 53,297 - -0.0 53,434 + +0.1 53,570 + -0.3 53,570 - +0.9 54,246 - -1.6 54,246 - +0.5 54,324 - -0.1 54,324 - +1.0
Or see this region's nucleotide sequence