Experiment: planktonic; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS05135 and LRK53_RS05140 are separated by 18 nucleotides LRK53_RS05140 and LRK53_RS05145 are separated by 13 nucleotides LRK53_RS05145 and LRK53_RS05150 overlap by 14 nucleotides LRK53_RS05150 and LRK53_RS05155 are separated by 8 nucleotides
LRK53_RS05135: LRK53_RS05135 - tol-pal system-associated acyl-CoA thioesterase, at 1,208,704 to 1,209,141
_RS05135
LRK53_RS05140: LRK53_RS05140 - protein TolQ, at 1,209,160 to 1,209,846
_RS05140
LRK53_RS05145: LRK53_RS05145 - protein TolR, at 1,209,860 to 1,210,324
_RS05145
LRK53_RS05150: LRK53_RS05150 - cell envelope integrity protein TolA, at 1,210,311 to 1,211,255
_RS05150
LRK53_RS05155: LRK53_RS05155 - Tol-Pal system beta propeller repeat protein TolB, at 1,211,264 to 1,212,586
_RS05155
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-2
-1
0
1 at 1208.985 kb on + strand, within LRK53_RS05135 at 1208.986 kb on - strand, within LRK53_RS05135 at 1208.986 kb on - strand, within LRK53_RS05135 at 1209.006 kb on + strand, within LRK53_RS05135 at 1209.139 kb on + strand at 1210.345 kb on + strand at 1210.345 kb on + strand at 1210.346 kb on - strand at 1210.346 kb on - strand at 1210.346 kb on - strand at 1210.346 kb on - strand at 1210.346 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction planktonic; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM remove 1,208,985 + LRK53_RS05135 0.64 -1.6 1,208,986 - LRK53_RS05135 0.64 -1.0 1,208,986 - LRK53_RS05135 0.64 -0.8 1,209,006 + LRK53_RS05135 0.69 +0.1 1,209,139 + -0.7 1,210,345 + -0.4 1,210,345 + -1.8 1,210,346 - -0.3 1,210,346 - -1.5 1,210,346 - -2.2 1,210,346 - +0.3 1,210,346 - -2.5
Or see this region's nucleotide sequence