Experiment: planktonic; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK53_RS04565 and LRK53_RS04570 are separated by 74 nucleotides LRK53_RS04570 and LRK53_RS04575 are separated by 17 nucleotides LRK53_RS04575 and LRK53_RS04580 overlap by 4 nucleotides LRK53_RS04580 and LRK53_RS04585 are separated by 130 nucleotides
LRK53_RS04565: LRK53_RS04565 - hypothetical protein, at 1,062,366 to 1,063,274
_RS04565
LRK53_RS04570: LRK53_RS04570 - DUF4097 family beta strand repeat-containing protein, at 1,063,349 to 1,064,251
_RS04570
LRK53_RS04575: LRK53_RS04575 - hypothetical protein, at 1,064,269 to 1,064,727
_RS04575
LRK53_RS04580: LRK53_RS04580 - sigma-70 family RNA polymerase sigma factor, at 1,064,724 to 1,065,269
_RS04580
LRK53_RS04585: LRK53_RS04585 - phosphoenolpyruvate carboxykinase (GTP), at 1,065,400 to 1,067,184
_RS04585
Position (kb)
1064
1065 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1065.031 kb on + strand, within LRK53_RS04580 at 1065.185 kb on - strand, within LRK53_RS04580 at 1065.515 kb on + strand at 1065.515 kb on + strand at 1065.515 kb on + strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand at 1065.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction planktonic; Day1; NLDM; Manganese (II) chloride tetrahydrate 10mM remove 1,065,031 + LRK53_RS04580 0.56 -2.2 1,065,185 - LRK53_RS04580 0.84 -0.0 1,065,515 + -0.8 1,065,515 + -1.8 1,065,515 + -1.1 1,065,516 - -0.8 1,065,516 - -3.1 1,065,516 - +1.4 1,065,516 - -1.4 1,065,516 - -0.9 1,065,516 - -2.3 1,065,516 - -1.0
Or see this region's nucleotide sequence