Experiment: monoculture; Experiment A, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03084 and MIT1002_03085 are separated by 135 nucleotides MIT1002_03085 and MIT1002_03086 are separated by 170 nucleotides
MIT1002_03084: MIT1002_03084 - Threonine synthase, at 3,435,433 to 3,436,716
_03084
MIT1002_03085: MIT1002_03085 - Homoserine kinase, at 3,436,852 to 3,437,796
_03085
MIT1002_03086: MIT1002_03086 - Aspartokinase I/homoserine dehydrogenase I, at 3,437,967 to 3,440,432
_03086
Position (kb)
3436
3437
3438 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 3435.967 kb on - strand, within MIT1002_03084 at 3435.967 kb on - strand, within MIT1002_03084 at 3436.094 kb on + strand, within MIT1002_03084 at 3436.096 kb on + strand, within MIT1002_03084 at 3436.104 kb on - strand, within MIT1002_03084 at 3436.104 kb on - strand, within MIT1002_03084 at 3436.104 kb on - strand, within MIT1002_03084 at 3436.116 kb on - strand, within MIT1002_03084 at 3436.119 kb on + strand, within MIT1002_03084 at 3436.119 kb on + strand, within MIT1002_03084 at 3436.127 kb on - strand, within MIT1002_03084 at 3436.195 kb on - strand, within MIT1002_03084 at 3436.240 kb on - strand, within MIT1002_03084 at 3436.246 kb on - strand, within MIT1002_03084 at 3436.290 kb on + strand, within MIT1002_03084 at 3436.431 kb on + strand, within MIT1002_03084 at 3436.439 kb on - strand, within MIT1002_03084 at 3436.514 kb on + strand, within MIT1002_03084 at 3436.517 kb on + strand, within MIT1002_03084 at 3436.519 kb on + strand, within MIT1002_03084 at 3436.537 kb on - strand, within MIT1002_03084 at 3436.551 kb on + strand, within MIT1002_03084 at 3436.556 kb on + strand, within MIT1002_03084 at 3436.559 kb on - strand, within MIT1002_03084 at 3436.654 kb on + strand at 3436.657 kb on + strand at 3436.738 kb on + strand at 3436.746 kb on - strand at 3436.746 kb on - strand at 3436.748 kb on - strand at 3436.753 kb on + strand at 3436.761 kb on - strand at 3436.868 kb on - strand at 3436.873 kb on + strand at 3436.888 kb on - strand at 3436.901 kb on - strand at 3437.133 kb on + strand, within MIT1002_03085 at 3437.181 kb on - strand, within MIT1002_03085 at 3437.197 kb on - strand, within MIT1002_03085 at 3437.199 kb on + strand, within MIT1002_03085 at 3437.216 kb on - strand, within MIT1002_03085 at 3437.236 kb on + strand, within MIT1002_03085 at 3437.258 kb on + strand, within MIT1002_03085 at 3437.266 kb on - strand, within MIT1002_03085 at 3437.266 kb on - strand, within MIT1002_03085 at 3437.266 kb on - strand, within MIT1002_03085 at 3437.266 kb on - strand, within MIT1002_03085 at 3437.317 kb on - strand, within MIT1002_03085 at 3437.531 kb on - strand, within MIT1002_03085 at 3437.540 kb on - strand, within MIT1002_03085 at 3437.608 kb on - strand, within MIT1002_03085 at 3437.705 kb on - strand at 3437.734 kb on - strand at 3437.932 kb on + strand at 3437.966 kb on - strand at 3438.017 kb on + strand at 3438.033 kb on - strand at 3438.068 kb on + strand at 3438.179 kb on + strand at 3438.234 kb on - strand, within MIT1002_03086 at 3438.272 kb on + strand, within MIT1002_03086 at 3438.280 kb on - strand, within MIT1002_03086 at 3438.294 kb on + strand, within MIT1002_03086 at 3438.294 kb on + strand, within MIT1002_03086 at 3438.302 kb on - strand, within MIT1002_03086 at 3438.381 kb on - strand, within MIT1002_03086 at 3438.405 kb on + strand, within MIT1002_03086 at 3438.413 kb on - strand, within MIT1002_03086 at 3438.475 kb on + strand, within MIT1002_03086 at 3438.483 kb on + strand, within MIT1002_03086 at 3438.485 kb on + strand, within MIT1002_03086 at 3438.538 kb on - strand, within MIT1002_03086 at 3438.540 kb on + strand, within MIT1002_03086 at 3438.548 kb on - strand, within MIT1002_03086 at 3438.596 kb on - strand, within MIT1002_03086 at 3438.596 kb on - strand, within MIT1002_03086 at 3438.601 kb on - strand, within MIT1002_03086 at 3438.629 kb on - strand, within MIT1002_03086 at 3438.646 kb on + strand, within MIT1002_03086 at 3438.656 kb on + strand, within MIT1002_03086 at 3438.664 kb on - strand, within MIT1002_03086 at 3438.664 kb on - strand, within MIT1002_03086 at 3438.664 kb on - strand, within MIT1002_03086 at 3438.672 kb on - strand, within MIT1002_03086 at 3438.679 kb on - strand, within MIT1002_03086
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 3 remove 3,435,967 - MIT1002_03084 0.42 -1.1 3,435,967 - MIT1002_03084 0.42 -2.9 3,436,094 + MIT1002_03084 0.51 +0.1 3,436,096 + MIT1002_03084 0.52 -3.8 3,436,104 - MIT1002_03084 0.52 -2.2 3,436,104 - MIT1002_03084 0.52 -0.5 3,436,104 - MIT1002_03084 0.52 -2.2 3,436,116 - MIT1002_03084 0.53 -0.4 3,436,119 + MIT1002_03084 0.53 -3.5 3,436,119 + MIT1002_03084 0.53 -3.5 3,436,127 - MIT1002_03084 0.54 -2.2 3,436,195 - MIT1002_03084 0.59 -0.8 3,436,240 - MIT1002_03084 0.63 -7.8 3,436,246 - MIT1002_03084 0.63 -2.4 3,436,290 + MIT1002_03084 0.67 -8.6 3,436,431 + MIT1002_03084 0.78 -2.1 3,436,439 - MIT1002_03084 0.78 -3.5 3,436,514 + MIT1002_03084 0.84 -2.2 3,436,517 + MIT1002_03084 0.84 -2.3 3,436,519 + MIT1002_03084 0.85 -1.5 3,436,537 - MIT1002_03084 0.86 -0.7 3,436,551 + MIT1002_03084 0.87 -2.6 3,436,556 + MIT1002_03084 0.87 -0.9 3,436,559 - MIT1002_03084 0.88 -3.7 3,436,654 + -0.7 3,436,657 + -2.1 3,436,738 + -1.7 3,436,746 - -3.2 3,436,746 - -1.6 3,436,748 - -1.8 3,436,753 + -3.8 3,436,761 - -3.5 3,436,868 - +0.1 3,436,873 + -3.1 3,436,888 - -2.6 3,436,901 - -2.9 3,437,133 + MIT1002_03085 0.30 -2.0 3,437,181 - MIT1002_03085 0.35 -5.9 3,437,197 - MIT1002_03085 0.37 -1.3 3,437,199 + MIT1002_03085 0.37 -1.9 3,437,216 - MIT1002_03085 0.39 -1.3 3,437,236 + MIT1002_03085 0.41 +0.6 3,437,258 + MIT1002_03085 0.43 -2.2 3,437,266 - MIT1002_03085 0.44 -1.4 3,437,266 - MIT1002_03085 0.44 -1.6 3,437,266 - MIT1002_03085 0.44 -4.3 3,437,266 - MIT1002_03085 0.44 -1.8 3,437,317 - MIT1002_03085 0.49 -3.3 3,437,531 - MIT1002_03085 0.72 -2.7 3,437,540 - MIT1002_03085 0.73 -2.9 3,437,608 - MIT1002_03085 0.80 -2.1 3,437,705 - -3.8 3,437,734 - -0.8 3,437,932 + -2.9 3,437,966 - +0.1 3,438,017 + -3.5 3,438,033 - -1.9 3,438,068 + -0.9 3,438,179 + -1.2 3,438,234 - MIT1002_03086 0.11 -2.1 3,438,272 + MIT1002_03086 0.12 -1.9 3,438,280 - MIT1002_03086 0.13 -3.1 3,438,294 + MIT1002_03086 0.13 -3.9 3,438,294 + MIT1002_03086 0.13 -3.9 3,438,302 - MIT1002_03086 0.14 -4.0 3,438,381 - MIT1002_03086 0.17 -2.4 3,438,405 + MIT1002_03086 0.18 -1.5 3,438,413 - MIT1002_03086 0.18 -4.2 3,438,475 + MIT1002_03086 0.21 -1.5 3,438,483 + MIT1002_03086 0.21 -5.6 3,438,485 + MIT1002_03086 0.21 -2.4 3,438,538 - MIT1002_03086 0.23 -1.8 3,438,540 + MIT1002_03086 0.23 -0.8 3,438,548 - MIT1002_03086 0.24 -5.3 3,438,596 - MIT1002_03086 0.26 -4.9 3,438,596 - MIT1002_03086 0.26 -3.7 3,438,601 - MIT1002_03086 0.26 -1.8 3,438,629 - MIT1002_03086 0.27 -5.0 3,438,646 + MIT1002_03086 0.28 -3.0 3,438,656 + MIT1002_03086 0.28 -0.9 3,438,664 - MIT1002_03086 0.28 -3.4 3,438,664 - MIT1002_03086 0.28 -3.6 3,438,664 - MIT1002_03086 0.28 -3.5 3,438,672 - MIT1002_03086 0.29 -1.7 3,438,679 - MIT1002_03086 0.29 -3.3
Or see this region's nucleotide sequence