Experiment: monoculture; Experiment A, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02720 and MIT1002_02721 are separated by 112 nucleotides MIT1002_02721 and MIT1002_02722 are separated by 13 nucleotides MIT1002_02722 and MIT1002_02723 are separated by 121 nucleotides MIT1002_02723 and MIT1002_02724 are separated by 187 nucleotides
MIT1002_02720: MIT1002_02720 - L-aspartate oxidase, at 3,022,925 to 3,024,610
_02720
MIT1002_02721: MIT1002_02721 - (3R)-hydroxyacyl-ACP dehydratase subunit HadB, at 3,024,723 to 3,025,595
_02721
MIT1002_02722: MIT1002_02722 - hypothetical protein, at 3,025,609 to 3,026,004
_02722
MIT1002_02723: MIT1002_02723 - Antitoxin CptB, at 3,026,126 to 3,026,377
_02723
MIT1002_02724: MIT1002_02724 - tRNA-modifying protein YgfZ, at 3,026,565 to 3,027,611
_02724
Position (kb)
3024
3025
3026 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3024.581 kb on + strand at 3024.624 kb on + strand at 3024.632 kb on - strand at 3024.721 kb on + strand at 3024.758 kb on + strand at 3024.758 kb on + strand at 3024.807 kb on - strand at 3024.886 kb on + strand, within MIT1002_02721 at 3024.921 kb on + strand, within MIT1002_02721 at 3024.939 kb on - strand, within MIT1002_02721 at 3024.946 kb on - strand, within MIT1002_02721 at 3024.976 kb on - strand, within MIT1002_02721 at 3025.010 kb on - strand, within MIT1002_02721 at 3025.012 kb on + strand, within MIT1002_02721 at 3025.012 kb on + strand, within MIT1002_02721 at 3025.012 kb on + strand, within MIT1002_02721 at 3025.012 kb on + strand, within MIT1002_02721 at 3025.020 kb on - strand, within MIT1002_02721 at 3025.031 kb on + strand, within MIT1002_02721 at 3025.032 kb on - strand, within MIT1002_02721 at 3025.032 kb on - strand, within MIT1002_02721 at 3025.041 kb on + strand, within MIT1002_02721 at 3025.051 kb on + strand, within MIT1002_02721 at 3025.059 kb on - strand, within MIT1002_02721 at 3025.059 kb on - strand, within MIT1002_02721 at 3025.142 kb on + strand, within MIT1002_02721 at 3025.150 kb on + strand, within MIT1002_02721 at 3025.150 kb on + strand, within MIT1002_02721 at 3025.158 kb on - strand, within MIT1002_02721 at 3025.165 kb on - strand, within MIT1002_02721 at 3025.181 kb on - strand, within MIT1002_02721 at 3025.277 kb on + strand, within MIT1002_02721 at 3025.418 kb on + strand, within MIT1002_02721 at 3025.509 kb on - strand at 3025.529 kb on + strand at 3025.536 kb on - strand at 3025.544 kb on + strand at 3025.560 kb on - strand at 3025.581 kb on - strand at 3025.606 kb on + strand at 3025.635 kb on - strand at 3025.662 kb on - strand, within MIT1002_02722 at 3025.687 kb on - strand, within MIT1002_02722 at 3025.753 kb on - strand, within MIT1002_02722 at 3025.755 kb on + strand, within MIT1002_02722 at 3025.763 kb on + strand, within MIT1002_02722 at 3025.815 kb on - strand, within MIT1002_02722 at 3025.870 kb on + strand, within MIT1002_02722 at 3025.915 kb on + strand, within MIT1002_02722 at 3025.923 kb on - strand, within MIT1002_02722 at 3026.022 kb on - strand at 3026.024 kb on + strand at 3026.042 kb on + strand at 3026.050 kb on - strand at 3026.050 kb on - strand at 3026.055 kb on - strand at 3026.131 kb on - strand at 3026.141 kb on + strand at 3026.149 kb on - strand at 3026.194 kb on - strand, within MIT1002_02723 at 3026.210 kb on + strand, within MIT1002_02723 at 3026.444 kb on + strand at 3026.471 kb on + strand at 3026.472 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 3 remove 3,024,581 + +1.9 3,024,624 + +1.7 3,024,632 - -1.0 3,024,721 + +0.4 3,024,758 + -0.3 3,024,758 + +0.9 3,024,807 - +0.0 3,024,886 + MIT1002_02721 0.19 +0.6 3,024,921 + MIT1002_02721 0.23 -1.1 3,024,939 - MIT1002_02721 0.25 -0.4 3,024,946 - MIT1002_02721 0.26 +0.3 3,024,976 - MIT1002_02721 0.29 +0.6 3,025,010 - MIT1002_02721 0.33 -1.7 3,025,012 + MIT1002_02721 0.33 -0.1 3,025,012 + MIT1002_02721 0.33 +1.8 3,025,012 + MIT1002_02721 0.33 +0.3 3,025,012 + MIT1002_02721 0.33 +1.6 3,025,020 - MIT1002_02721 0.34 +0.6 3,025,031 + MIT1002_02721 0.35 +0.7 3,025,032 - MIT1002_02721 0.35 +0.6 3,025,032 - MIT1002_02721 0.35 +2.1 3,025,041 + MIT1002_02721 0.36 +1.0 3,025,051 + MIT1002_02721 0.38 +0.2 3,025,059 - MIT1002_02721 0.38 -2.3 3,025,059 - MIT1002_02721 0.38 +0.9 3,025,142 + MIT1002_02721 0.48 +1.5 3,025,150 + MIT1002_02721 0.49 -2.0 3,025,150 + MIT1002_02721 0.49 -0.8 3,025,158 - MIT1002_02721 0.50 +0.7 3,025,165 - MIT1002_02721 0.51 +0.2 3,025,181 - MIT1002_02721 0.52 +0.3 3,025,277 + MIT1002_02721 0.63 +1.7 3,025,418 + MIT1002_02721 0.80 +2.4 3,025,509 - -1.3 3,025,529 + -0.2 3,025,536 - -1.3 3,025,544 + +0.3 3,025,560 - +3.2 3,025,581 - -0.5 3,025,606 + -1.5 3,025,635 - -0.0 3,025,662 - MIT1002_02722 0.13 +0.4 3,025,687 - MIT1002_02722 0.20 -0.8 3,025,753 - MIT1002_02722 0.36 -2.3 3,025,755 + MIT1002_02722 0.37 -1.8 3,025,763 + MIT1002_02722 0.39 +0.7 3,025,815 - MIT1002_02722 0.52 -0.4 3,025,870 + MIT1002_02722 0.66 -0.3 3,025,915 + MIT1002_02722 0.77 -0.4 3,025,923 - MIT1002_02722 0.79 +0.3 3,026,022 - +0.1 3,026,024 + -2.0 3,026,042 + -0.5 3,026,050 - +0.7 3,026,050 - +0.9 3,026,055 - -0.8 3,026,131 - +0.7 3,026,141 + +1.0 3,026,149 - +2.0 3,026,194 - MIT1002_02723 0.27 -1.1 3,026,210 + MIT1002_02723 0.33 +0.6 3,026,444 + -1.4 3,026,471 + -0.4 3,026,472 + -0.2
Or see this region's nucleotide sequence