Experiment: monoculture; Experiment A, time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02172 and MIT1002_02173 are separated by 76 nucleotides MIT1002_02173 and MIT1002_02174 are separated by 110 nucleotides MIT1002_02174 and MIT1002_02175 are separated by 296 nucleotides
MIT1002_02172: MIT1002_02172 - Protein required for attachment to host cells, at 2,425,420 to 2,425,857
_02172
MIT1002_02173: MIT1002_02173 - hypothetical protein, at 2,425,934 to 2,426,647
_02173
MIT1002_02174: MIT1002_02174 - Colicin I receptor precursor, at 2,426,758 to 2,429,664
_02174
MIT1002_02175: MIT1002_02175 - Transposase, at 2,429,961 to 2,430,476
_02175
Position (kb)
2426
2427
2428
2429
2430 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2425.786 kb on - strand, within MIT1002_02172 at 2425.791 kb on - strand, within MIT1002_02172 at 2425.958 kb on - strand at 2425.959 kb on + strand at 2425.959 kb on + strand at 2425.995 kb on + strand at 2426.011 kb on + strand, within MIT1002_02173 at 2426.165 kb on + strand, within MIT1002_02173 at 2426.165 kb on + strand, within MIT1002_02173 at 2426.188 kb on - strand, within MIT1002_02173 at 2426.201 kb on - strand, within MIT1002_02173 at 2426.219 kb on + strand, within MIT1002_02173 at 2426.273 kb on + strand, within MIT1002_02173 at 2426.364 kb on - strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.404 kb on + strand, within MIT1002_02173 at 2426.411 kb on + strand, within MIT1002_02173 at 2426.412 kb on - strand, within MIT1002_02173 at 2426.412 kb on - strand, within MIT1002_02173 at 2426.569 kb on - strand, within MIT1002_02173 at 2426.583 kb on - strand at 2426.583 kb on - strand at 2426.583 kb on - strand at 2426.589 kb on + strand at 2426.597 kb on - strand at 2426.608 kb on + strand at 2426.616 kb on - strand at 2426.630 kb on - strand at 2426.632 kb on + strand at 2426.656 kb on + strand at 2426.683 kb on - strand at 2426.780 kb on - strand at 2426.872 kb on - strand at 2426.961 kb on + strand at 2427.033 kb on + strand at 2427.064 kb on - strand, within MIT1002_02174 at 2427.069 kb on + strand, within MIT1002_02174 at 2427.084 kb on - strand, within MIT1002_02174 at 2427.093 kb on + strand, within MIT1002_02174 at 2427.118 kb on + strand, within MIT1002_02174 at 2427.126 kb on - strand, within MIT1002_02174 at 2427.126 kb on - strand, within MIT1002_02174 at 2427.135 kb on + strand, within MIT1002_02174 at 2427.212 kb on - strand, within MIT1002_02174 at 2427.212 kb on - strand, within MIT1002_02174 at 2427.212 kb on - strand, within MIT1002_02174 at 2427.212 kb on - strand, within MIT1002_02174 at 2427.215 kb on - strand, within MIT1002_02174 at 2427.249 kb on + strand, within MIT1002_02174 at 2427.250 kb on - strand, within MIT1002_02174 at 2427.262 kb on + strand, within MIT1002_02174 at 2427.270 kb on - strand, within MIT1002_02174 at 2427.339 kb on + strand, within MIT1002_02174 at 2427.340 kb on + strand, within MIT1002_02174 at 2427.347 kb on - strand, within MIT1002_02174 at 2427.348 kb on + strand, within MIT1002_02174 at 2427.358 kb on + strand, within MIT1002_02174 at 2427.456 kb on - strand, within MIT1002_02174 at 2427.584 kb on - strand, within MIT1002_02174 at 2427.584 kb on - strand, within MIT1002_02174 at 2427.584 kb on - strand, within MIT1002_02174 at 2427.633 kb on + strand, within MIT1002_02174 at 2427.633 kb on + strand, within MIT1002_02174 at 2427.635 kb on + strand, within MIT1002_02174 at 2427.641 kb on - strand, within MIT1002_02174 at 2427.641 kb on - strand, within MIT1002_02174 at 2427.758 kb on + strand, within MIT1002_02174 at 2427.773 kb on + strand, within MIT1002_02174 at 2427.783 kb on + strand, within MIT1002_02174 at 2427.791 kb on - strand, within MIT1002_02174 at 2427.791 kb on - strand, within MIT1002_02174 at 2427.797 kb on + strand, within MIT1002_02174 at 2427.905 kb on - strand, within MIT1002_02174 at 2427.925 kb on + strand, within MIT1002_02174 at 2427.925 kb on + strand, within MIT1002_02174 at 2427.991 kb on + strand, within MIT1002_02174 at 2427.992 kb on - strand, within MIT1002_02174 at 2428.009 kb on - strand, within MIT1002_02174 at 2428.030 kb on + strand, within MIT1002_02174 at 2428.030 kb on + strand, within MIT1002_02174 at 2428.072 kb on - strand, within MIT1002_02174 at 2428.114 kb on - strand, within MIT1002_02174 at 2428.132 kb on + strand, within MIT1002_02174 at 2428.132 kb on + strand, within MIT1002_02174 at 2428.151 kb on + strand, within MIT1002_02174 at 2428.159 kb on - strand, within MIT1002_02174 at 2428.258 kb on - strand, within MIT1002_02174 at 2428.331 kb on - strand, within MIT1002_02174 at 2428.345 kb on + strand, within MIT1002_02174 at 2428.345 kb on + strand, within MIT1002_02174 at 2428.345 kb on + strand, within MIT1002_02174 at 2428.380 kb on + strand, within MIT1002_02174 at 2428.388 kb on - strand, within MIT1002_02174 at 2428.503 kb on + strand, within MIT1002_02174 at 2428.503 kb on + strand, within MIT1002_02174 at 2428.547 kb on - strand, within MIT1002_02174 at 2428.699 kb on - strand, within MIT1002_02174 at 2428.814 kb on + strand, within MIT1002_02174 at 2428.890 kb on + strand, within MIT1002_02174 at 2428.910 kb on - strand, within MIT1002_02174 at 2428.926 kb on + strand, within MIT1002_02174 at 2428.944 kb on - strand, within MIT1002_02174 at 2428.944 kb on - strand, within MIT1002_02174 at 2428.965 kb on + strand, within MIT1002_02174 at 2428.965 kb on + strand, within MIT1002_02174 at 2429.061 kb on - strand, within MIT1002_02174 at 2429.080 kb on + strand, within MIT1002_02174 at 2429.090 kb on + strand, within MIT1002_02174 at 2429.090 kb on + strand, within MIT1002_02174 at 2429.097 kb on + strand, within MIT1002_02174 at 2429.156 kb on - strand, within MIT1002_02174 at 2429.241 kb on + strand, within MIT1002_02174 at 2429.272 kb on + strand, within MIT1002_02174 at 2429.315 kb on + strand, within MIT1002_02174 at 2429.333 kb on - strand, within MIT1002_02174 at 2429.343 kb on - strand, within MIT1002_02174 at 2429.343 kb on - strand, within MIT1002_02174 at 2429.343 kb on - strand, within MIT1002_02174 at 2429.350 kb on + strand, within MIT1002_02174 at 2429.350 kb on + strand, within MIT1002_02174 at 2429.350 kb on + strand, within MIT1002_02174 at 2429.358 kb on - strand, within MIT1002_02174 at 2429.358 kb on - strand, within MIT1002_02174 at 2429.358 kb on - strand, within MIT1002_02174 at 2429.442 kb on - strand at 2429.495 kb on + strand at 2429.513 kb on - strand at 2429.596 kb on - strand at 2429.614 kb on + strand at 2429.614 kb on + strand at 2429.635 kb on + strand at 2429.664 kb on + strand at 2429.672 kb on - strand at 2429.815 kb on + strand at 2429.853 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 3 remove 2,425,786 - MIT1002_02172 0.84 -0.6 2,425,791 - MIT1002_02172 0.85 +1.8 2,425,958 - -0.6 2,425,959 + -1.9 2,425,959 + -0.1 2,425,995 + +1.0 2,426,011 + MIT1002_02173 0.11 -0.4 2,426,165 + MIT1002_02173 0.32 -0.4 2,426,165 + MIT1002_02173 0.32 +0.3 2,426,188 - MIT1002_02173 0.36 -0.6 2,426,201 - MIT1002_02173 0.37 -0.9 2,426,219 + MIT1002_02173 0.40 -1.3 2,426,273 + MIT1002_02173 0.47 +1.7 2,426,364 - MIT1002_02173 0.60 -0.3 2,426,404 + MIT1002_02173 0.66 +1.6 2,426,404 + MIT1002_02173 0.66 -0.3 2,426,404 + MIT1002_02173 0.66 -0.1 2,426,404 + MIT1002_02173 0.66 +0.4 2,426,404 + MIT1002_02173 0.66 -0.1 2,426,404 + MIT1002_02173 0.66 +1.1 2,426,404 + MIT1002_02173 0.66 +0.0 2,426,404 + MIT1002_02173 0.66 -1.5 2,426,404 + MIT1002_02173 0.66 +0.3 2,426,411 + MIT1002_02173 0.67 +0.9 2,426,412 - MIT1002_02173 0.67 +0.9 2,426,412 - MIT1002_02173 0.67 +0.5 2,426,569 - MIT1002_02173 0.89 -0.1 2,426,583 - +1.2 2,426,583 - -1.4 2,426,583 - -0.8 2,426,589 + -0.2 2,426,597 - -0.0 2,426,608 + -0.1 2,426,616 - -0.6 2,426,630 - -0.2 2,426,632 + -0.4 2,426,656 + +0.5 2,426,683 - +0.9 2,426,780 - -1.2 2,426,872 - -0.1 2,426,961 + +0.2 2,427,033 + +0.3 2,427,064 - MIT1002_02174 0.11 +1.0 2,427,069 + MIT1002_02174 0.11 +0.8 2,427,084 - MIT1002_02174 0.11 -0.3 2,427,093 + MIT1002_02174 0.12 -0.1 2,427,118 + MIT1002_02174 0.12 +0.4 2,427,126 - MIT1002_02174 0.13 +0.1 2,427,126 - MIT1002_02174 0.13 -0.3 2,427,135 + MIT1002_02174 0.13 +1.6 2,427,212 - MIT1002_02174 0.16 -0.2 2,427,212 - MIT1002_02174 0.16 -0.5 2,427,212 - MIT1002_02174 0.16 +0.4 2,427,212 - MIT1002_02174 0.16 -0.9 2,427,215 - MIT1002_02174 0.16 -0.3 2,427,249 + MIT1002_02174 0.17 +0.7 2,427,250 - MIT1002_02174 0.17 +0.9 2,427,262 + MIT1002_02174 0.17 -0.6 2,427,270 - MIT1002_02174 0.18 -0.7 2,427,339 + MIT1002_02174 0.20 -0.4 2,427,340 + MIT1002_02174 0.20 +0.9 2,427,347 - MIT1002_02174 0.20 +1.3 2,427,348 + MIT1002_02174 0.20 -0.4 2,427,358 + MIT1002_02174 0.21 -0.5 2,427,456 - MIT1002_02174 0.24 -0.4 2,427,584 - MIT1002_02174 0.28 -0.2 2,427,584 - MIT1002_02174 0.28 -1.3 2,427,584 - MIT1002_02174 0.28 +0.5 2,427,633 + MIT1002_02174 0.30 -2.5 2,427,633 + MIT1002_02174 0.30 +0.0 2,427,635 + MIT1002_02174 0.30 -1.6 2,427,641 - MIT1002_02174 0.30 -1.3 2,427,641 - MIT1002_02174 0.30 +0.5 2,427,758 + MIT1002_02174 0.34 -1.1 2,427,773 + MIT1002_02174 0.35 -0.9 2,427,783 + MIT1002_02174 0.35 +0.4 2,427,791 - MIT1002_02174 0.36 -1.1 2,427,791 - MIT1002_02174 0.36 +0.1 2,427,797 + MIT1002_02174 0.36 -1.6 2,427,905 - MIT1002_02174 0.39 +1.6 2,427,925 + MIT1002_02174 0.40 +0.5 2,427,925 + MIT1002_02174 0.40 -0.7 2,427,991 + MIT1002_02174 0.42 -0.9 2,427,992 - MIT1002_02174 0.42 +0.9 2,428,009 - MIT1002_02174 0.43 -2.3 2,428,030 + MIT1002_02174 0.44 +1.5 2,428,030 + MIT1002_02174 0.44 -1.1 2,428,072 - MIT1002_02174 0.45 +1.2 2,428,114 - MIT1002_02174 0.47 +0.0 2,428,132 + MIT1002_02174 0.47 -1.0 2,428,132 + MIT1002_02174 0.47 +0.1 2,428,151 + MIT1002_02174 0.48 -0.1 2,428,159 - MIT1002_02174 0.48 +0.3 2,428,258 - MIT1002_02174 0.52 +0.6 2,428,331 - MIT1002_02174 0.54 +0.6 2,428,345 + MIT1002_02174 0.55 -1.6 2,428,345 + MIT1002_02174 0.55 +0.2 2,428,345 + MIT1002_02174 0.55 -1.0 2,428,380 + MIT1002_02174 0.56 -0.1 2,428,388 - MIT1002_02174 0.56 -0.9 2,428,503 + MIT1002_02174 0.60 -0.1 2,428,503 + MIT1002_02174 0.60 -1.0 2,428,547 - MIT1002_02174 0.62 +0.7 2,428,699 - MIT1002_02174 0.67 +0.5 2,428,814 + MIT1002_02174 0.71 -0.5 2,428,890 + MIT1002_02174 0.73 -0.4 2,428,910 - MIT1002_02174 0.74 -2.4 2,428,926 + MIT1002_02174 0.75 +0.5 2,428,944 - MIT1002_02174 0.75 +0.1 2,428,944 - MIT1002_02174 0.75 -1.4 2,428,965 + MIT1002_02174 0.76 -1.2 2,428,965 + MIT1002_02174 0.76 -0.3 2,429,061 - MIT1002_02174 0.79 -1.1 2,429,080 + MIT1002_02174 0.80 -0.5 2,429,090 + MIT1002_02174 0.80 +0.5 2,429,090 + MIT1002_02174 0.80 +1.5 2,429,097 + MIT1002_02174 0.80 -1.0 2,429,156 - MIT1002_02174 0.82 -1.0 2,429,241 + MIT1002_02174 0.85 -0.1 2,429,272 + MIT1002_02174 0.86 -0.3 2,429,315 + MIT1002_02174 0.88 +0.1 2,429,333 - MIT1002_02174 0.89 -0.8 2,429,343 - MIT1002_02174 0.89 -0.8 2,429,343 - MIT1002_02174 0.89 +0.9 2,429,343 - MIT1002_02174 0.89 -1.2 2,429,350 + MIT1002_02174 0.89 +0.8 2,429,350 + MIT1002_02174 0.89 -0.7 2,429,350 + MIT1002_02174 0.89 +1.7 2,429,358 - MIT1002_02174 0.89 +0.2 2,429,358 - MIT1002_02174 0.89 +0.2 2,429,358 - MIT1002_02174 0.89 +0.8 2,429,442 - +0.1 2,429,495 + -0.4 2,429,513 - +1.1 2,429,596 - +0.1 2,429,614 + +1.1 2,429,614 + -0.6 2,429,635 + +1.8 2,429,664 + +0.9 2,429,672 - -1.4 2,429,815 + +2.9 2,429,853 + -0.2
Or see this region's nucleotide sequence