Strain Fitness in Alteromonas macleodii MIT1002 around MIT1002_00107

Experiment: monoculture; Experiment A, time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMIT1002_00106 and MIT1002_00107 are separated by 169 nucleotidesMIT1002_00107 and MIT1002_00108 are separated by 142 nucleotides MIT1002_00106: MIT1002_00106 - Guanylate kinase, at 114,744 to 115,385 _00106 MIT1002_00107: MIT1002_00107 - DNA-directed RNA polymerase subunit omega, at 115,555 to 115,827 _00107 MIT1002_00108: MIT1002_00108 - Bifunctional (p)ppGpp synthase/hydrolase SpoT, at 115,970 to 118,081 _00108 Position (kb) 115 116Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 114.557 kb on + strandat 114.611 kb on + strandat 114.611 kb on + strandat 114.624 kb on + strandat 114.625 kb on + strandat 114.625 kb on + strandat 114.652 kb on - strandat 114.671 kb on + strandat 114.682 kb on - strandat 115.378 kb on - strandat 115.378 kb on - strandat 115.453 kb on - strandat 115.508 kb on - strandat 115.576 kb on - strandat 115.576 kb on - strandat 115.601 kb on + strand, within MIT1002_00107at 115.621 kb on + strand, within MIT1002_00107at 115.691 kb on - strand, within MIT1002_00107at 115.741 kb on - strand, within MIT1002_00107at 115.809 kb on + strandat 115.817 kb on - strandat 115.918 kb on + strandat 115.938 kb on + strandat 115.956 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 3
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114,557 + +1.8
114,611 + +0.0
114,611 + -0.1
114,624 + -0.1
114,625 + +0.0
114,625 + +0.6
114,652 - -1.0
114,671 + -0.7
114,682 - -0.1
115,378 - -1.4
115,378 - -3.3
115,453 - -0.2
115,508 - -2.0
115,576 - -1.1
115,576 - -1.3
115,601 + MIT1002_00107 0.17 +1.8
115,621 + MIT1002_00107 0.24 -1.3
115,691 - MIT1002_00107 0.50 -2.7
115,741 - MIT1002_00107 0.68 -1.0
115,809 + -0.7
115,817 - +0.0
115,918 + -0.6
115,938 + +1.6
115,956 + -0.9

Or see this region's nucleotide sequence