Experiment: monoculture; Experiment A, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_03002 and MIT1002_03003 are separated by 21 nucleotides MIT1002_03003 and MIT1002_03004 are separated by 210 nucleotides
MIT1002_03002: MIT1002_03002 - Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, at 3,343,696 to 3,345,747
_03002
MIT1002_03003: MIT1002_03003 - Pyruvate dehydrogenase E1 component, at 3,345,769 to 3,348,444
_03003
MIT1002_03004: MIT1002_03004 - Pyruvate dehydrogenase complex repressor, at 3,348,655 to 3,349,401
_03004
Position (kb)
3345
3346
3347
3348
3349 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3345.078 kb on + strand, within MIT1002_03002 at 3345.254 kb on + strand, within MIT1002_03002 at 3345.409 kb on + strand, within MIT1002_03002 at 3346.079 kb on + strand, within MIT1002_03003 at 3346.271 kb on - strand, within MIT1002_03003 at 3346.342 kb on - strand, within MIT1002_03003 at 3346.599 kb on - strand, within MIT1002_03003 at 3346.685 kb on + strand, within MIT1002_03003 at 3346.716 kb on + strand, within MIT1002_03003 at 3346.959 kb on - strand, within MIT1002_03003 at 3347.043 kb on + strand, within MIT1002_03003 at 3347.068 kb on + strand, within MIT1002_03003 at 3347.076 kb on - strand, within MIT1002_03003 at 3347.473 kb on + strand, within MIT1002_03003 at 3347.700 kb on - strand, within MIT1002_03003 at 3347.781 kb on + strand, within MIT1002_03003 at 3347.799 kb on - strand, within MIT1002_03003 at 3347.804 kb on + strand, within MIT1002_03003 at 3347.834 kb on + strand, within MIT1002_03003 at 3348.087 kb on - strand, within MIT1002_03003 at 3348.105 kb on - strand, within MIT1002_03003 at 3348.138 kb on + strand, within MIT1002_03003 at 3348.202 kb on + strand at 3348.545 kb on + strand at 3348.593 kb on - strand at 3348.593 kb on - strand at 3348.628 kb on - strand at 3348.628 kb on - strand at 3348.710 kb on - strand at 3348.710 kb on - strand at 3348.733 kb on + strand, within MIT1002_03004 at 3348.772 kb on - strand, within MIT1002_03004 at 3348.828 kb on - strand, within MIT1002_03004 at 3348.838 kb on - strand, within MIT1002_03004 at 3348.838 kb on - strand, within MIT1002_03004 at 3348.838 kb on - strand, within MIT1002_03004 at 3348.877 kb on - strand, within MIT1002_03004 at 3348.880 kb on - strand, within MIT1002_03004 at 3348.923 kb on - strand, within MIT1002_03004 at 3348.940 kb on - strand, within MIT1002_03004 at 3348.971 kb on + strand, within MIT1002_03004 at 3349.004 kb on - strand, within MIT1002_03004 at 3349.013 kb on - strand, within MIT1002_03004 at 3349.015 kb on + strand, within MIT1002_03004 at 3349.023 kb on - strand, within MIT1002_03004 at 3349.044 kb on - strand, within MIT1002_03004 at 3349.044 kb on - strand, within MIT1002_03004 at 3349.062 kb on + strand, within MIT1002_03004 at 3349.070 kb on - strand, within MIT1002_03004 at 3349.137 kb on + strand, within MIT1002_03004 at 3349.167 kb on - strand, within MIT1002_03004 at 3349.180 kb on - strand, within MIT1002_03004 at 3349.209 kb on + strand, within MIT1002_03004 at 3349.233 kb on - strand, within MIT1002_03004 at 3349.273 kb on - strand, within MIT1002_03004 at 3349.320 kb on - strand, within MIT1002_03004 at 3349.330 kb on - strand at 3349.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 2 remove 3,345,078 + MIT1002_03002 0.67 -1.8 3,345,254 + MIT1002_03002 0.76 -0.9 3,345,409 + MIT1002_03002 0.83 -2.4 3,346,079 + MIT1002_03003 0.12 -0.6 3,346,271 - MIT1002_03003 0.19 -0.9 3,346,342 - MIT1002_03003 0.21 -1.3 3,346,599 - MIT1002_03003 0.31 -2.4 3,346,685 + MIT1002_03003 0.34 +0.2 3,346,716 + MIT1002_03003 0.35 -0.6 3,346,959 - MIT1002_03003 0.44 -0.8 3,347,043 + MIT1002_03003 0.48 -1.6 3,347,068 + MIT1002_03003 0.49 -2.2 3,347,076 - MIT1002_03003 0.49 -1.6 3,347,473 + MIT1002_03003 0.64 -1.6 3,347,700 - MIT1002_03003 0.72 -3.5 3,347,781 + MIT1002_03003 0.75 -1.2 3,347,799 - MIT1002_03003 0.76 -1.3 3,347,804 + MIT1002_03003 0.76 -2.2 3,347,834 + MIT1002_03003 0.77 -0.8 3,348,087 - MIT1002_03003 0.87 +1.2 3,348,105 - MIT1002_03003 0.87 +0.2 3,348,138 + MIT1002_03003 0.89 -0.3 3,348,202 + +0.5 3,348,545 + -2.0 3,348,593 - -0.0 3,348,593 - -2.1 3,348,628 - -1.4 3,348,628 - +0.4 3,348,710 - -0.4 3,348,710 - -1.8 3,348,733 + MIT1002_03004 0.10 -0.1 3,348,772 - MIT1002_03004 0.16 -1.7 3,348,828 - MIT1002_03004 0.23 +0.7 3,348,838 - MIT1002_03004 0.24 -0.1 3,348,838 - MIT1002_03004 0.24 -2.7 3,348,838 - MIT1002_03004 0.24 -0.4 3,348,877 - MIT1002_03004 0.30 -1.0 3,348,880 - MIT1002_03004 0.30 +0.2 3,348,923 - MIT1002_03004 0.36 +1.5 3,348,940 - MIT1002_03004 0.38 -1.7 3,348,971 + MIT1002_03004 0.42 -1.3 3,349,004 - MIT1002_03004 0.47 -1.3 3,349,013 - MIT1002_03004 0.48 -0.2 3,349,015 + MIT1002_03004 0.48 +0.2 3,349,023 - MIT1002_03004 0.49 -2.0 3,349,044 - MIT1002_03004 0.52 +0.7 3,349,044 - MIT1002_03004 0.52 +0.1 3,349,062 + MIT1002_03004 0.54 -1.2 3,349,070 - MIT1002_03004 0.56 -1.0 3,349,137 + MIT1002_03004 0.65 -0.1 3,349,167 - MIT1002_03004 0.69 -0.6 3,349,180 - MIT1002_03004 0.70 +0.4 3,349,209 + MIT1002_03004 0.74 +1.2 3,349,233 - MIT1002_03004 0.77 -0.9 3,349,273 - MIT1002_03004 0.83 -0.1 3,349,320 - MIT1002_03004 0.89 -0.5 3,349,330 - -0.7 3,349,405 - -1.8
Or see this region's nucleotide sequence