Experiment: monoculture; Experiment A, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02254 and MIT1002_02255 are separated by 63 nucleotides MIT1002_02255 and MIT1002_02256 are separated by 26 nucleotides MIT1002_02256 and MIT1002_02257 are separated by 13 nucleotides MIT1002_02257 and MIT1002_02258 overlap by 11 nucleotides
MIT1002_02254: MIT1002_02254 - hypothetical protein, at 2,509,988 to 2,510,221
_02254
MIT1002_02255: MIT1002_02255 - hypothetical protein, at 2,510,285 to 2,511,139
_02255
MIT1002_02256: MIT1002_02256 - hypothetical protein, at 2,511,166 to 2,511,534
_02256
MIT1002_02257: MIT1002_02257 - hypothetical protein, at 2,511,548 to 2,511,925
_02257
MIT1002_02258: MIT1002_02258 - putative isomerase YddE, at 2,511,915 to 2,512,706
_02258
Position (kb)
2511
2512 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2510.239 kb on + strand at 2510.239 kb on + strand at 2510.260 kb on - strand at 2510.260 kb on - strand at 2510.299 kb on - strand at 2510.342 kb on + strand at 2510.348 kb on + strand at 2510.348 kb on + strand at 2510.348 kb on + strand at 2510.348 kb on + strand at 2510.350 kb on + strand at 2510.350 kb on - strand at 2510.356 kb on - strand at 2510.356 kb on - strand at 2510.356 kb on - strand at 2510.357 kb on - strand at 2510.393 kb on + strand, within MIT1002_02255 at 2510.395 kb on + strand, within MIT1002_02255 at 2510.395 kb on + strand, within MIT1002_02255 at 2510.395 kb on + strand, within MIT1002_02255 at 2510.395 kb on + strand, within MIT1002_02255 at 2510.395 kb on + strand, within MIT1002_02255 at 2510.403 kb on + strand, within MIT1002_02255 at 2510.403 kb on - strand, within MIT1002_02255 at 2510.403 kb on - strand, within MIT1002_02255 at 2510.403 kb on - strand, within MIT1002_02255 at 2510.417 kb on - strand, within MIT1002_02255 at 2510.549 kb on + strand, within MIT1002_02255 at 2510.551 kb on - strand, within MIT1002_02255 at 2510.562 kb on - strand, within MIT1002_02255 at 2510.588 kb on + strand, within MIT1002_02255 at 2510.623 kb on + strand, within MIT1002_02255 at 2510.631 kb on + strand, within MIT1002_02255 at 2510.631 kb on - strand, within MIT1002_02255 at 2510.655 kb on - strand, within MIT1002_02255 at 2510.655 kb on - strand, within MIT1002_02255 at 2510.687 kb on - strand, within MIT1002_02255 at 2510.724 kb on + strand, within MIT1002_02255 at 2510.792 kb on + strand, within MIT1002_02255 at 2510.792 kb on + strand, within MIT1002_02255 at 2510.800 kb on - strand, within MIT1002_02255 at 2510.806 kb on - strand, within MIT1002_02255 at 2510.814 kb on + strand, within MIT1002_02255 at 2510.835 kb on - strand, within MIT1002_02255 at 2510.849 kb on - strand, within MIT1002_02255 at 2510.895 kb on - strand, within MIT1002_02255 at 2510.932 kb on + strand, within MIT1002_02255 at 2510.932 kb on + strand, within MIT1002_02255 at 2510.940 kb on - strand, within MIT1002_02255 at 2511.014 kb on + strand, within MIT1002_02255 at 2511.022 kb on - strand, within MIT1002_02255 at 2511.044 kb on + strand, within MIT1002_02255 at 2511.045 kb on + strand, within MIT1002_02255 at 2511.090 kb on - strand at 2511.141 kb on - strand at 2511.163 kb on + strand at 2511.163 kb on + strand at 2511.202 kb on + strand at 2511.204 kb on + strand, within MIT1002_02256 at 2511.207 kb on - strand, within MIT1002_02256 at 2511.209 kb on + strand, within MIT1002_02256 at 2511.209 kb on + strand, within MIT1002_02256 at 2511.212 kb on + strand, within MIT1002_02256 at 2511.212 kb on - strand, within MIT1002_02256 at 2511.244 kb on - strand, within MIT1002_02256 at 2511.360 kb on + strand, within MIT1002_02256 at 2511.366 kb on - strand, within MIT1002_02256 at 2511.366 kb on - strand, within MIT1002_02256 at 2511.368 kb on - strand, within MIT1002_02256 at 2511.368 kb on - strand, within MIT1002_02256 at 2511.368 kb on - strand, within MIT1002_02256 at 2511.368 kb on - strand, within MIT1002_02256 at 2511.409 kb on - strand, within MIT1002_02256 at 2511.523 kb on - strand at 2511.537 kb on - strand at 2511.537 kb on - strand at 2511.537 kb on - strand at 2511.537 kb on - strand at 2511.648 kb on - strand, within MIT1002_02257 at 2511.655 kb on - strand, within MIT1002_02257 at 2511.690 kb on + strand, within MIT1002_02257 at 2511.848 kb on - strand, within MIT1002_02257 at 2511.864 kb on - strand, within MIT1002_02257 at 2511.959 kb on + strand at 2512.312 kb on + strand, within MIT1002_02258 at 2512.388 kb on - strand, within MIT1002_02258 at 2512.404 kb on + strand, within MIT1002_02258 at 2512.404 kb on + strand, within MIT1002_02258 at 2512.404 kb on + strand, within MIT1002_02258 at 2512.456 kb on - strand, within MIT1002_02258 at 2512.523 kb on - strand, within MIT1002_02258 at 2512.534 kb on + strand, within MIT1002_02258
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 2 remove 2,510,239 + -0.6 2,510,239 + -0.6 2,510,260 - -2.2 2,510,260 - -1.2 2,510,299 - -0.2 2,510,342 + -2.7 2,510,348 + -0.9 2,510,348 + -0.6 2,510,348 + +1.0 2,510,348 + +0.6 2,510,350 + -0.7 2,510,350 - -1.9 2,510,356 - -0.6 2,510,356 - +0.9 2,510,356 - +0.7 2,510,357 - -0.1 2,510,393 + MIT1002_02255 0.13 +1.0 2,510,395 + MIT1002_02255 0.13 +0.2 2,510,395 + MIT1002_02255 0.13 +1.2 2,510,395 + MIT1002_02255 0.13 -0.3 2,510,395 + MIT1002_02255 0.13 +1.4 2,510,395 + MIT1002_02255 0.13 -1.9 2,510,403 + MIT1002_02255 0.14 -0.4 2,510,403 - MIT1002_02255 0.14 +1.4 2,510,403 - MIT1002_02255 0.14 -0.1 2,510,403 - MIT1002_02255 0.14 +0.2 2,510,417 - MIT1002_02255 0.15 +1.8 2,510,549 + MIT1002_02255 0.31 -0.7 2,510,551 - MIT1002_02255 0.31 -0.6 2,510,562 - MIT1002_02255 0.32 -0.3 2,510,588 + MIT1002_02255 0.35 -0.4 2,510,623 + MIT1002_02255 0.40 -0.3 2,510,631 + MIT1002_02255 0.40 +0.6 2,510,631 - MIT1002_02255 0.40 -0.8 2,510,655 - MIT1002_02255 0.43 +0.1 2,510,655 - MIT1002_02255 0.43 +0.6 2,510,687 - MIT1002_02255 0.47 -1.3 2,510,724 + MIT1002_02255 0.51 -0.5 2,510,792 + MIT1002_02255 0.59 +0.2 2,510,792 + MIT1002_02255 0.59 +0.6 2,510,800 - MIT1002_02255 0.60 -2.5 2,510,806 - MIT1002_02255 0.61 -0.0 2,510,814 + MIT1002_02255 0.62 -1.4 2,510,835 - MIT1002_02255 0.64 +0.3 2,510,849 - MIT1002_02255 0.66 -2.0 2,510,895 - MIT1002_02255 0.71 -1.1 2,510,932 + MIT1002_02255 0.76 +0.8 2,510,932 + MIT1002_02255 0.76 -1.5 2,510,940 - MIT1002_02255 0.77 -0.5 2,511,014 + MIT1002_02255 0.85 +0.3 2,511,022 - MIT1002_02255 0.86 -0.2 2,511,044 + MIT1002_02255 0.89 +0.3 2,511,045 + MIT1002_02255 0.89 -0.2 2,511,090 - +0.3 2,511,141 - +0.1 2,511,163 + +0.1 2,511,163 + -0.3 2,511,202 + -0.6 2,511,204 + MIT1002_02256 0.10 -1.0 2,511,207 - MIT1002_02256 0.11 +0.8 2,511,209 + MIT1002_02256 0.12 +0.3 2,511,209 + MIT1002_02256 0.12 -0.5 2,511,212 + MIT1002_02256 0.12 -0.0 2,511,212 - MIT1002_02256 0.12 -0.2 2,511,244 - MIT1002_02256 0.21 +0.7 2,511,360 + MIT1002_02256 0.53 +0.2 2,511,366 - MIT1002_02256 0.54 +1.3 2,511,366 - MIT1002_02256 0.54 +0.4 2,511,368 - MIT1002_02256 0.55 +0.8 2,511,368 - MIT1002_02256 0.55 -1.5 2,511,368 - MIT1002_02256 0.55 +0.7 2,511,368 - MIT1002_02256 0.55 +0.2 2,511,409 - MIT1002_02256 0.66 +0.7 2,511,523 - -0.7 2,511,537 - +0.9 2,511,537 - -0.7 2,511,537 - -0.2 2,511,537 - -1.0 2,511,648 - MIT1002_02257 0.26 -1.1 2,511,655 - MIT1002_02257 0.28 -0.1 2,511,690 + MIT1002_02257 0.38 +0.2 2,511,848 - MIT1002_02257 0.79 -0.1 2,511,864 - MIT1002_02257 0.84 +1.3 2,511,959 + -0.5 2,512,312 + MIT1002_02258 0.50 -0.9 2,512,388 - MIT1002_02258 0.60 -0.4 2,512,404 + MIT1002_02258 0.62 -3.7 2,512,404 + MIT1002_02258 0.62 +0.0 2,512,404 + MIT1002_02258 0.62 -1.5 2,512,456 - MIT1002_02258 0.68 +0.8 2,512,523 - MIT1002_02258 0.77 -1.3 2,512,534 + MIT1002_02258 0.78 -1.4
Or see this region's nucleotide sequence