Experiment: monoculture; Experiment A, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02016 and MIT1002_02017 overlap by 8 nucleotides MIT1002_02017 and MIT1002_02018 are separated by 10 nucleotides MIT1002_02018 and MIT1002_02020 are separated by 520 nucleotides
MIT1002_02016: MIT1002_02016 - Serine/threonine-protein kinase A, at 2,257,926 to 2,259,806
_02016
MIT1002_02017: MIT1002_02017 - Acyl-CoA thioesterase 2, at 2,259,799 to 2,260,659
_02017
MIT1002_02018: MIT1002_02018 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase, at 2,260,670 to 2,261,194
_02018
MIT1002_02020: MIT1002_02020 - Purine catabolism protein PucG, at 2,261,715 to 2,262,836
_02020
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2259.680 kb on - strand at 2259.696 kb on + strand at 2259.915 kb on - strand, within MIT1002_02017 at 2260.065 kb on + strand, within MIT1002_02017 at 2260.065 kb on + strand, within MIT1002_02017 at 2260.073 kb on - strand, within MIT1002_02017 at 2260.073 kb on - strand, within MIT1002_02017 at 2260.136 kb on - strand, within MIT1002_02017 at 2260.188 kb on + strand, within MIT1002_02017 at 2260.196 kb on - strand, within MIT1002_02017 at 2260.246 kb on + strand, within MIT1002_02017 at 2260.246 kb on + strand, within MIT1002_02017 at 2260.256 kb on + strand, within MIT1002_02017 at 2260.280 kb on + strand, within MIT1002_02017 at 2260.288 kb on - strand, within MIT1002_02017 at 2260.345 kb on - strand, within MIT1002_02017 at 2260.345 kb on - strand, within MIT1002_02017 at 2260.365 kb on + strand, within MIT1002_02017 at 2260.395 kb on - strand, within MIT1002_02017 at 2260.424 kb on - strand, within MIT1002_02017 at 2260.442 kb on + strand, within MIT1002_02017 at 2260.442 kb on + strand, within MIT1002_02017 at 2260.450 kb on - strand, within MIT1002_02017 at 2260.450 kb on - strand, within MIT1002_02017 at 2260.478 kb on + strand, within MIT1002_02017 at 2260.563 kb on + strand, within MIT1002_02017 at 2260.563 kb on + strand, within MIT1002_02017 at 2260.563 kb on + strand, within MIT1002_02017 at 2260.571 kb on - strand, within MIT1002_02017 at 2260.571 kb on - strand, within MIT1002_02017 at 2260.668 kb on - strand at 2260.673 kb on - strand at 2260.738 kb on - strand, within MIT1002_02018 at 2260.773 kb on - strand, within MIT1002_02018 at 2260.775 kb on - strand, within MIT1002_02018 at 2260.812 kb on + strand, within MIT1002_02018 at 2260.820 kb on - strand, within MIT1002_02018 at 2260.895 kb on + strand, within MIT1002_02018 at 2260.902 kb on + strand, within MIT1002_02018 at 2260.916 kb on - strand, within MIT1002_02018 at 2260.930 kb on - strand, within MIT1002_02018 at 2260.973 kb on + strand, within MIT1002_02018 at 2260.981 kb on - strand, within MIT1002_02018 at 2261.007 kb on - strand, within MIT1002_02018 at 2261.014 kb on + strand, within MIT1002_02018 at 2261.152 kb on + strand at 2261.160 kb on - strand at 2261.209 kb on - strand at 2261.409 kb on - strand at 2261.409 kb on - strand at 2261.518 kb on + strand at 2261.526 kb on - strand at 2261.609 kb on + strand at 2261.619 kb on - strand at 2261.630 kb on - strand at 2261.641 kb on - strand at 2261.761 kb on + strand at 2261.761 kb on + strand at 2261.761 kb on + strand at 2261.769 kb on - strand at 2261.769 kb on - strand at 2261.769 kb on - strand at 2261.769 kb on - strand at 2261.769 kb on - strand at 2261.769 kb on - strand at 2261.804 kb on + strand at 2261.819 kb on + strand at 2261.819 kb on + strand at 2261.857 kb on + strand, within MIT1002_02020 at 2261.867 kb on + strand, within MIT1002_02020 at 2261.987 kb on - strand, within MIT1002_02020 at 2262.033 kb on - strand, within MIT1002_02020 at 2262.047 kb on + strand, within MIT1002_02020 at 2262.047 kb on + strand, within MIT1002_02020 at 2262.055 kb on - strand, within MIT1002_02020 at 2262.055 kb on - strand, within MIT1002_02020 at 2262.085 kb on + strand, within MIT1002_02020 at 2262.086 kb on + strand, within MIT1002_02020 at 2262.093 kb on - strand, within MIT1002_02020
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 2 remove 2,259,680 - -0.3 2,259,696 + +0.7 2,259,915 - MIT1002_02017 0.13 +0.6 2,260,065 + MIT1002_02017 0.31 +0.3 2,260,065 + MIT1002_02017 0.31 +0.1 2,260,073 - MIT1002_02017 0.32 +0.5 2,260,073 - MIT1002_02017 0.32 -0.2 2,260,136 - MIT1002_02017 0.39 +0.7 2,260,188 + MIT1002_02017 0.45 -1.3 2,260,196 - MIT1002_02017 0.46 +0.2 2,260,246 + MIT1002_02017 0.52 -1.2 2,260,246 + MIT1002_02017 0.52 +0.7 2,260,256 + MIT1002_02017 0.53 +1.3 2,260,280 + MIT1002_02017 0.56 -1.1 2,260,288 - MIT1002_02017 0.57 +0.7 2,260,345 - MIT1002_02017 0.63 -1.7 2,260,345 - MIT1002_02017 0.63 -0.7 2,260,365 + MIT1002_02017 0.66 -0.4 2,260,395 - MIT1002_02017 0.69 +0.0 2,260,424 - MIT1002_02017 0.73 -0.5 2,260,442 + MIT1002_02017 0.75 +0.2 2,260,442 + MIT1002_02017 0.75 +1.3 2,260,450 - MIT1002_02017 0.76 -1.7 2,260,450 - MIT1002_02017 0.76 -0.6 2,260,478 + MIT1002_02017 0.79 -0.0 2,260,563 + MIT1002_02017 0.89 -1.1 2,260,563 + MIT1002_02017 0.89 -0.6 2,260,563 + MIT1002_02017 0.89 -0.6 2,260,571 - MIT1002_02017 0.90 +1.7 2,260,571 - MIT1002_02017 0.90 +0.8 2,260,668 - +1.3 2,260,673 - +1.0 2,260,738 - MIT1002_02018 0.13 +0.9 2,260,773 - MIT1002_02018 0.20 +0.8 2,260,775 - MIT1002_02018 0.20 +2.6 2,260,812 + MIT1002_02018 0.27 -2.3 2,260,820 - MIT1002_02018 0.29 -0.2 2,260,895 + MIT1002_02018 0.43 +0.6 2,260,902 + MIT1002_02018 0.44 +1.0 2,260,916 - MIT1002_02018 0.47 -0.3 2,260,930 - MIT1002_02018 0.50 +0.6 2,260,973 + MIT1002_02018 0.58 +1.1 2,260,981 - MIT1002_02018 0.59 +0.9 2,261,007 - MIT1002_02018 0.64 -0.2 2,261,014 + MIT1002_02018 0.66 -0.5 2,261,152 + -0.2 2,261,160 - -0.6 2,261,209 - +0.3 2,261,409 - -0.3 2,261,409 - +1.7 2,261,518 + -0.5 2,261,526 - -2.8 2,261,609 + -1.4 2,261,619 - -0.8 2,261,630 - +1.2 2,261,641 - +0.7 2,261,761 + -2.8 2,261,761 + -0.1 2,261,761 + +0.1 2,261,769 - +1.0 2,261,769 - -0.4 2,261,769 - -0.8 2,261,769 - -0.3 2,261,769 - -0.4 2,261,769 - -1.7 2,261,804 + +0.4 2,261,819 + +1.2 2,261,819 + +0.6 2,261,857 + MIT1002_02020 0.13 -0.2 2,261,867 + MIT1002_02020 0.14 -0.2 2,261,987 - MIT1002_02020 0.24 -1.4 2,262,033 - MIT1002_02020 0.28 -0.4 2,262,047 + MIT1002_02020 0.30 -0.2 2,262,047 + MIT1002_02020 0.30 -0.2 2,262,055 - MIT1002_02020 0.30 -0.9 2,262,055 - MIT1002_02020 0.30 -2.0 2,262,085 + MIT1002_02020 0.33 -0.1 2,262,086 + MIT1002_02020 0.33 -0.5 2,262,093 - MIT1002_02020 0.34 +0.9
Or see this region's nucleotide sequence