Experiment: monoculture; Experiment A, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01397 and MIT1002_01398 are separated by 246 nucleotides MIT1002_01398 and MIT1002_01399 are separated by 37 nucleotides MIT1002_01399 and MIT1002_01400 are separated by 37 nucleotides MIT1002_01400 and MIT1002_01401 are separated by 38 nucleotides
MIT1002_01397: MIT1002_01397 - DNA-binding transcriptional regulator BasR, at 1,547,748 to 1,549,370
_01397
MIT1002_01398: MIT1002_01398 - tRNA-Val, at 1,549,617 to 1,549,692
_01398
MIT1002_01399: MIT1002_01399 - tRNA-Val, at 1,549,730 to 1,549,805
_01399
MIT1002_01400: MIT1002_01400 - tRNA-Val, at 1,549,843 to 1,549,918
_01400
MIT1002_01401: MIT1002_01401 - tRNA-Val, at 1,549,957 to 1,550,032
_01401
Position (kb)
1549
1550 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1548.812 kb on - strand, within MIT1002_01397 at 1548.814 kb on + strand, within MIT1002_01397 at 1548.815 kb on + strand, within MIT1002_01397 at 1548.855 kb on - strand, within MIT1002_01397 at 1548.892 kb on - strand, within MIT1002_01397 at 1548.923 kb on - strand, within MIT1002_01397 at 1549.012 kb on - strand, within MIT1002_01397 at 1549.064 kb on - strand, within MIT1002_01397 at 1549.087 kb on - strand, within MIT1002_01397 at 1549.124 kb on - strand, within MIT1002_01397 at 1549.228 kb on + strand at 1549.236 kb on - strand at 1549.236 kb on - strand at 1549.306 kb on - strand at 1549.390 kb on + strand at 1549.422 kb on + strand at 1549.433 kb on - strand at 1549.445 kb on + strand at 1549.466 kb on + strand at 1549.526 kb on + strand at 1549.545 kb on + strand at 1549.634 kb on - strand, within MIT1002_01398 at 1549.718 kb on - strand at 1549.721 kb on + strand at 1549.754 kb on - strand, within MIT1002_01399 at 1550.146 kb on + strand at 1550.252 kb on - strand at 1550.266 kb on - strand at 1550.361 kb on + strand at 1550.369 kb on - strand at 1550.473 kb on - strand at 1550.473 kb on - strand at 1550.538 kb on + strand at 1550.538 kb on + strand at 1550.600 kb on - strand at 1550.603 kb on + strand at 1550.603 kb on + strand at 1550.611 kb on - strand at 1550.695 kb on - strand at 1550.711 kb on - strand at 1550.794 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 2 remove 1,548,812 - MIT1002_01397 0.66 +0.2 1,548,814 + MIT1002_01397 0.66 -0.2 1,548,815 + MIT1002_01397 0.66 -1.9 1,548,855 - MIT1002_01397 0.68 +0.1 1,548,892 - MIT1002_01397 0.70 -1.1 1,548,923 - MIT1002_01397 0.72 -0.5 1,549,012 - MIT1002_01397 0.78 -0.0 1,549,064 - MIT1002_01397 0.81 -0.4 1,549,087 - MIT1002_01397 0.83 +1.9 1,549,124 - MIT1002_01397 0.85 -0.6 1,549,228 + -0.1 1,549,236 - -0.2 1,549,236 - -0.4 1,549,306 - -0.7 1,549,390 + +0.3 1,549,422 + +1.3 1,549,433 - +1.1 1,549,445 + +0.9 1,549,466 + +0.4 1,549,526 + -0.2 1,549,545 + +0.3 1,549,634 - MIT1002_01398 0.22 -0.4 1,549,718 - +2.0 1,549,721 + -0.3 1,549,754 - MIT1002_01399 0.32 -0.2 1,550,146 + -0.5 1,550,252 - +0.3 1,550,266 - +0.2 1,550,361 + +1.6 1,550,369 - -0.6 1,550,473 - -1.3 1,550,473 - +0.8 1,550,538 + +0.3 1,550,538 + +0.6 1,550,600 - -1.4 1,550,603 + -0.3 1,550,603 + +0.7 1,550,611 - -1.5 1,550,695 - -0.4 1,550,711 - +0.2 1,550,794 + -0.2
Or see this region's nucleotide sequence