Experiment: monoculture; Experiment A, time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_00063 and MIT1002_00064 overlap by 4 nucleotides MIT1002_00064 and MIT1002_00065 overlap by 1 nucleotides MIT1002_00065 and MIT1002_00066 are separated by 141 nucleotides
MIT1002_00063: MIT1002_00063 - 2-amino-3-ketobutyrate coenzyme A ligase, at 68,853 to 70,049
_00063
MIT1002_00064: MIT1002_00064 - L-threonine 3-dehydrogenase, at 70,046 to 71,071
_00064
MIT1002_00065: MIT1002_00065 - Thiosulfate sulfurtransferase GlpE, at 71,071 to 71,382
_00065
MIT1002_00066: MIT1002_00066 - Rhomboid protease GlpG, at 71,524 to 72,387
_00066
Position (kb)
70
71
72 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 69.054 kb on + strand, within MIT1002_00063 at 69.162 kb on - strand, within MIT1002_00063 at 69.224 kb on - strand, within MIT1002_00063 at 69.257 kb on - strand, within MIT1002_00063 at 69.269 kb on + strand, within MIT1002_00063 at 69.277 kb on + strand, within MIT1002_00063 at 69.287 kb on + strand, within MIT1002_00063 at 69.287 kb on + strand, within MIT1002_00063 at 69.307 kb on - strand, within MIT1002_00063 at 69.344 kb on + strand, within MIT1002_00063 at 69.466 kb on - strand, within MIT1002_00063 at 69.526 kb on + strand, within MIT1002_00063 at 69.533 kb on + strand, within MIT1002_00063 at 69.548 kb on - strand, within MIT1002_00063 at 69.585 kb on + strand, within MIT1002_00063 at 69.585 kb on + strand, within MIT1002_00063 at 69.590 kb on + strand, within MIT1002_00063 at 69.593 kb on - strand, within MIT1002_00063 at 69.593 kb on - strand, within MIT1002_00063 at 69.607 kb on + strand, within MIT1002_00063 at 69.715 kb on - strand, within MIT1002_00063 at 69.761 kb on - strand, within MIT1002_00063 at 69.761 kb on - strand, within MIT1002_00063 at 69.917 kb on + strand, within MIT1002_00063 at 69.925 kb on - strand, within MIT1002_00063 at 69.933 kb on - strand at 69.939 kb on + strand at 69.939 kb on + strand at 69.939 kb on + strand at 69.947 kb on - strand at 69.962 kb on + strand at 69.972 kb on + strand at 69.979 kb on + strand at 69.979 kb on + strand at 69.980 kb on - strand at 69.981 kb on - strand at 69.987 kb on - strand at 70.093 kb on + strand at 70.105 kb on + strand at 70.124 kb on - strand at 70.217 kb on + strand, within MIT1002_00064 at 70.278 kb on + strand, within MIT1002_00064 at 70.278 kb on + strand, within MIT1002_00064 at 70.278 kb on + strand, within MIT1002_00064 at 70.278 kb on + strand, within MIT1002_00064 at 70.279 kb on + strand, within MIT1002_00064 at 70.279 kb on + strand, within MIT1002_00064 at 70.286 kb on - strand, within MIT1002_00064 at 70.286 kb on - strand, within MIT1002_00064 at 70.286 kb on - strand, within MIT1002_00064 at 70.286 kb on - strand, within MIT1002_00064 at 70.349 kb on - strand, within MIT1002_00064 at 70.380 kb on + strand, within MIT1002_00064 at 70.478 kb on - strand, within MIT1002_00064 at 70.505 kb on + strand, within MIT1002_00064 at 70.557 kb on - strand, within MIT1002_00064 at 70.605 kb on - strand, within MIT1002_00064 at 70.630 kb on + strand, within MIT1002_00064 at 70.644 kb on + strand, within MIT1002_00064 at 70.652 kb on - strand, within MIT1002_00064 at 70.803 kb on - strand, within MIT1002_00064 at 70.828 kb on + strand, within MIT1002_00064 at 70.839 kb on - strand, within MIT1002_00064 at 70.918 kb on + strand, within MIT1002_00064 at 70.967 kb on - strand, within MIT1002_00064 at 71.039 kb on - strand at 71.039 kb on - strand at 71.122 kb on + strand, within MIT1002_00065 at 71.130 kb on - strand, within MIT1002_00065 at 71.149 kb on + strand, within MIT1002_00065 at 71.149 kb on + strand, within MIT1002_00065 at 71.170 kb on - strand, within MIT1002_00065 at 71.175 kb on + strand, within MIT1002_00065 at 71.175 kb on + strand, within MIT1002_00065 at 71.254 kb on + strand, within MIT1002_00065 at 71.403 kb on - strand at 71.453 kb on + strand at 71.461 kb on - strand at 71.461 kb on - strand at 71.479 kb on - strand at 71.609 kb on + strand at 71.609 kb on + strand at 71.617 kb on - strand, within MIT1002_00066 at 71.617 kb on - strand, within MIT1002_00066 at 71.617 kb on - strand, within MIT1002_00066 at 71.617 kb on - strand, within MIT1002_00066 at 71.651 kb on + strand, within MIT1002_00066 at 71.659 kb on + strand, within MIT1002_00066 at 71.659 kb on + strand, within MIT1002_00066 at 71.703 kb on + strand, within MIT1002_00066 at 71.826 kb on - strand, within MIT1002_00066 at 72.019 kb on - strand, within MIT1002_00066 at 72.064 kb on - strand, within MIT1002_00066
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 2 remove 69,054 + MIT1002_00063 0.17 -2.8 69,162 - MIT1002_00063 0.26 +0.3 69,224 - MIT1002_00063 0.31 +0.2 69,257 - MIT1002_00063 0.34 +0.5 69,269 + MIT1002_00063 0.35 +0.5 69,277 + MIT1002_00063 0.35 -0.6 69,287 + MIT1002_00063 0.36 -0.4 69,287 + MIT1002_00063 0.36 +1.8 69,307 - MIT1002_00063 0.38 -0.2 69,344 + MIT1002_00063 0.41 -0.1 69,466 - MIT1002_00063 0.51 -0.3 69,526 + MIT1002_00063 0.56 +2.7 69,533 + MIT1002_00063 0.57 +0.7 69,548 - MIT1002_00063 0.58 +0.4 69,585 + MIT1002_00063 0.61 +0.3 69,585 + MIT1002_00063 0.61 +0.5 69,590 + MIT1002_00063 0.62 -0.8 69,593 - MIT1002_00063 0.62 +0.6 69,593 - MIT1002_00063 0.62 +1.5 69,607 + MIT1002_00063 0.63 -1.2 69,715 - MIT1002_00063 0.72 -0.3 69,761 - MIT1002_00063 0.76 +0.1 69,761 - MIT1002_00063 0.76 +0.5 69,917 + MIT1002_00063 0.89 -1.0 69,925 - MIT1002_00063 0.90 -0.6 69,933 - +0.1 69,939 + -1.0 69,939 + +0.8 69,939 + -1.4 69,947 - +0.1 69,962 + -0.4 69,972 + -3.0 69,979 + -0.1 69,979 + -0.5 69,980 - +0.0 69,981 - +0.3 69,987 - -1.4 70,093 + -0.9 70,105 + +1.2 70,124 - -0.7 70,217 + MIT1002_00064 0.17 +1.5 70,278 + MIT1002_00064 0.23 -1.5 70,278 + MIT1002_00064 0.23 -0.3 70,278 + MIT1002_00064 0.23 -0.6 70,278 + MIT1002_00064 0.23 -0.2 70,279 + MIT1002_00064 0.23 -0.7 70,279 + MIT1002_00064 0.23 +0.5 70,286 - MIT1002_00064 0.23 +0.3 70,286 - MIT1002_00064 0.23 +2.9 70,286 - MIT1002_00064 0.23 -0.6 70,286 - MIT1002_00064 0.23 -0.2 70,349 - MIT1002_00064 0.30 +0.1 70,380 + MIT1002_00064 0.33 -0.1 70,478 - MIT1002_00064 0.42 +0.1 70,505 + MIT1002_00064 0.45 +0.6 70,557 - MIT1002_00064 0.50 -0.6 70,605 - MIT1002_00064 0.54 +0.1 70,630 + MIT1002_00064 0.57 -1.0 70,644 + MIT1002_00064 0.58 -0.7 70,652 - MIT1002_00064 0.59 -0.2 70,803 - MIT1002_00064 0.74 +0.8 70,828 + MIT1002_00064 0.76 -0.1 70,839 - MIT1002_00064 0.77 -0.8 70,918 + MIT1002_00064 0.85 +0.4 70,967 - MIT1002_00064 0.90 -1.2 71,039 - -0.4 71,039 - -0.3 71,122 + MIT1002_00065 0.16 +0.1 71,130 - MIT1002_00065 0.19 -1.1 71,149 + MIT1002_00065 0.25 +0.1 71,149 + MIT1002_00065 0.25 +0.3 71,170 - MIT1002_00065 0.32 -1.5 71,175 + MIT1002_00065 0.33 +1.1 71,175 + MIT1002_00065 0.33 -0.1 71,254 + MIT1002_00065 0.59 +0.1 71,403 - +1.0 71,453 + +1.7 71,461 - +0.9 71,461 - -0.1 71,479 - +0.7 71,609 + -0.2 71,609 + -1.0 71,617 - MIT1002_00066 0.11 -1.8 71,617 - MIT1002_00066 0.11 -1.3 71,617 - MIT1002_00066 0.11 +0.5 71,617 - MIT1002_00066 0.11 -0.4 71,651 + MIT1002_00066 0.15 -1.3 71,659 + MIT1002_00066 0.16 -0.3 71,659 + MIT1002_00066 0.16 -0.2 71,703 + MIT1002_00066 0.21 -1.1 71,826 - MIT1002_00066 0.35 +0.1 72,019 - MIT1002_00066 0.57 -1.0 72,064 - MIT1002_00066 0.62 -1.4
Or see this region's nucleotide sequence