Strain Fitness in Alteromonas macleodii MIT1002 around MIT1002_00005

Experiment: monoculture; Experiment A, time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMIT1002_00004 and MIT1002_00005 are separated by 171 nucleotidesMIT1002_00005 and MIT1002_00006 are separated by 85 nucleotides MIT1002_00004: MIT1002_00004 - DNA gyrase subunit B, at 3,949 to 6,369 _00004 MIT1002_00005: MIT1002_00005 - hypothetical protein, at 6,541 to 6,984 _00005 MIT1002_00006: MIT1002_00006 - Glycine--tRNA ligase beta subunit, at 7,070 to 9,148 _00006 Position (kb) 6 7Strain fitness (log2 ratio) -1 0 1 2at 6.364 kb on + strandat 6.428 kb on + strandat 6.436 kb on - strandat 6.526 kb on + strandat 6.538 kb on + strandat 6.538 kb on + strandat 6.538 kb on + strandat 6.611 kb on + strand, within MIT1002_00005at 6.611 kb on + strand, within MIT1002_00005at 6.619 kb on - strand, within MIT1002_00005at 6.623 kb on - strand, within MIT1002_00005at 6.715 kb on - strand, within MIT1002_00005at 6.723 kb on - strand, within MIT1002_00005at 6.751 kb on + strand, within MIT1002_00005at 6.751 kb on + strand, within MIT1002_00005at 6.759 kb on - strand, within MIT1002_00005at 6.759 kb on - strand, within MIT1002_00005at 6.833 kb on + strand, within MIT1002_00005at 6.852 kb on - strand, within MIT1002_00005at 6.891 kb on + strand, within MIT1002_00005at 7.013 kb on + strandat 7.021 kb on - strandat 7.021 kb on - strandat 7.031 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 2
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6,364 + -1.3
6,428 + -1.0
6,436 - +0.4
6,526 + -1.0
6,538 + -1.2
6,538 + +0.3
6,538 + +1.0
6,611 + MIT1002_00005 0.16 +2.1
6,611 + MIT1002_00005 0.16 +1.7
6,619 - MIT1002_00005 0.18 +1.3
6,623 - MIT1002_00005 0.18 +0.1
6,715 - MIT1002_00005 0.39 +1.6
6,723 - MIT1002_00005 0.41 +0.5
6,751 + MIT1002_00005 0.47 +1.8
6,751 + MIT1002_00005 0.47 -0.8
6,759 - MIT1002_00005 0.49 -0.1
6,759 - MIT1002_00005 0.49 +0.0
6,833 + MIT1002_00005 0.66 +0.2
6,852 - MIT1002_00005 0.70 +1.0
6,891 + MIT1002_00005 0.79 -0.5
7,013 + -0.3
7,021 - -0.8
7,021 - +0.3
7,031 - +0.1

Or see this region's nucleotide sequence