Experiment: monoculture; Experiment A, time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_02520 and MIT1002_02521 are separated by 216 nucleotides MIT1002_02521 and MIT1002_02522 are separated by 48 nucleotides MIT1002_02522 and MIT1002_02523 are separated by 65 nucleotides MIT1002_02523 and MIT1002_02524 are separated by 101 nucleotides
MIT1002_02520: MIT1002_02520 - tRNA-(MS[2]IO[6]A)-hydroxylase (MiaE), at 2,810,899 to 2,811,675
_02520
MIT1002_02521: MIT1002_02521 - Inner membrane protein YohC, at 2,811,892 to 2,812,491
_02521
MIT1002_02522: MIT1002_02522 - putative transposase OrfB, at 2,812,540 to 2,813,307
_02522
MIT1002_02523: MIT1002_02523 - hypothetical protein, at 2,813,373 to 2,813,768
_02523
MIT1002_02524: MIT1002_02524 - Amidophosphoribosyltransferase, at 2,813,870 to 2,815,393
_02524
Position (kb)
2812
2813
2814 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 2811.545 kb on + strand, within MIT1002_02520 at 2811.545 kb on + strand, within MIT1002_02520 at 2811.545 kb on + strand, within MIT1002_02520 at 2811.545 kb on + strand, within MIT1002_02520 at 2811.545 kb on + strand, within MIT1002_02520 at 2811.545 kb on + strand, within MIT1002_02520 at 2811.553 kb on - strand, within MIT1002_02520 at 2811.553 kb on - strand, within MIT1002_02520 at 2811.595 kb on + strand, within MIT1002_02520 at 2811.600 kb on + strand at 2811.684 kb on - strand at 2811.781 kb on - strand at 2811.782 kb on + strand at 2811.800 kb on - strand at 2811.809 kb on + strand at 2811.821 kb on + strand at 2812.162 kb on + strand, within MIT1002_02521 at 2812.199 kb on + strand, within MIT1002_02521 at 2812.199 kb on + strand, within MIT1002_02521 at 2812.207 kb on - strand, within MIT1002_02521 at 2812.207 kb on - strand, within MIT1002_02521 at 2812.211 kb on + strand, within MIT1002_02521 at 2812.233 kb on - strand, within MIT1002_02521 at 2812.240 kb on - strand, within MIT1002_02521 at 2812.240 kb on - strand, within MIT1002_02521 at 2812.240 kb on - strand, within MIT1002_02521 at 2812.278 kb on + strand, within MIT1002_02521 at 2812.278 kb on + strand, within MIT1002_02521 at 2812.318 kb on - strand, within MIT1002_02521 at 2812.337 kb on + strand, within MIT1002_02521 at 2812.343 kb on - strand, within MIT1002_02521 at 2812.345 kb on - strand, within MIT1002_02521 at 2812.345 kb on - strand, within MIT1002_02521 at 2812.394 kb on + strand, within MIT1002_02521 at 2813.780 kb on + strand at 2813.862 kb on + strand at 2813.896 kb on + strand at 2813.896 kb on + strand at 2813.904 kb on - strand at 2813.919 kb on - strand at 2813.981 kb on - strand at 2814.005 kb on - strand at 2814.140 kb on + strand, within MIT1002_02524 at 2814.263 kb on + strand, within MIT1002_02524 at 2814.274 kb on + strand, within MIT1002_02524 at 2814.274 kb on + strand, within MIT1002_02524 at 2814.274 kb on + strand, within MIT1002_02524 at 2814.274 kb on + strand, within MIT1002_02524 at 2814.282 kb on - strand, within MIT1002_02524 at 2814.297 kb on - strand, within MIT1002_02524 at 2814.307 kb on + strand, within MIT1002_02524 at 2814.307 kb on + strand, within MIT1002_02524
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 1 remove 2,811,545 + MIT1002_02520 0.83 +0.7 2,811,545 + MIT1002_02520 0.83 +0.7 2,811,545 + MIT1002_02520 0.83 -2.9 2,811,545 + MIT1002_02520 0.83 -0.7 2,811,545 + MIT1002_02520 0.83 -0.1 2,811,545 + MIT1002_02520 0.83 -3.1 2,811,553 - MIT1002_02520 0.84 -0.2 2,811,553 - MIT1002_02520 0.84 -0.7 2,811,595 + MIT1002_02520 0.90 -0.5 2,811,600 + -1.2 2,811,684 - +2.6 2,811,781 - -0.2 2,811,782 + -1.1 2,811,800 - +0.5 2,811,809 + -1.1 2,811,821 + -1.1 2,812,162 + MIT1002_02521 0.45 -1.3 2,812,199 + MIT1002_02521 0.51 +0.3 2,812,199 + MIT1002_02521 0.51 +0.2 2,812,207 - MIT1002_02521 0.53 +0.6 2,812,207 - MIT1002_02521 0.53 -0.1 2,812,211 + MIT1002_02521 0.53 -0.4 2,812,233 - MIT1002_02521 0.57 -1.8 2,812,240 - MIT1002_02521 0.58 +0.3 2,812,240 - MIT1002_02521 0.58 +0.8 2,812,240 - MIT1002_02521 0.58 +2.6 2,812,278 + MIT1002_02521 0.64 +0.3 2,812,278 + MIT1002_02521 0.64 -0.1 2,812,318 - MIT1002_02521 0.71 -0.2 2,812,337 + MIT1002_02521 0.74 +0.2 2,812,343 - MIT1002_02521 0.75 +0.4 2,812,345 - MIT1002_02521 0.76 -0.4 2,812,345 - MIT1002_02521 0.76 +0.9 2,812,394 + MIT1002_02521 0.84 +1.4 2,813,780 + -0.8 2,813,862 + +0.2 2,813,896 + -2.7 2,813,896 + -0.3 2,813,904 - -2.2 2,813,919 - -0.4 2,813,981 - -0.5 2,814,005 - -4.7 2,814,140 + MIT1002_02524 0.18 -1.7 2,814,263 + MIT1002_02524 0.26 -1.2 2,814,274 + MIT1002_02524 0.27 -4.2 2,814,274 + MIT1002_02524 0.27 -7.7 2,814,274 + MIT1002_02524 0.27 -2.1 2,814,274 + MIT1002_02524 0.27 -4.4 2,814,282 - MIT1002_02524 0.27 -3.5 2,814,297 - MIT1002_02524 0.28 -1.9 2,814,307 + MIT1002_02524 0.29 -4.4 2,814,307 + MIT1002_02524 0.29 -3.5
Or see this region's nucleotide sequence