Experiment: monoculture; Experiment A, time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MIT1002_01402 and MIT1002_01403 are separated by 85 nucleotides MIT1002_01403 and MIT1002_01404 are separated by 93 nucleotides MIT1002_01404 and MIT1002_01405 are separated by 157 nucleotides MIT1002_01405 and MIT1002_01406 are separated by 157 nucleotides MIT1002_01406 and MIT1002_01407 are separated by 157 nucleotides
MIT1002_01402: MIT1002_01402 - Glutamate--tRNA ligase, at 1,550,936 to 1,552,345
_01402
MIT1002_01403: MIT1002_01403 - tRNA-Ala, at 1,552,431 to 1,552,506
_01403
MIT1002_01404: MIT1002_01404 - tRNA-Glu, at 1,552,600 to 1,552,675
_01404
MIT1002_01405: MIT1002_01405 - tRNA-Glu, at 1,552,833 to 1,552,908
_01405
MIT1002_01406: MIT1002_01406 - tRNA-Glu, at 1,553,066 to 1,553,141
_01406
MIT1002_01407: MIT1002_01407 - tRNA-Glu, at 1,553,299 to 1,553,374
_01407
Position (kb)
1552
1553 Strain fitness (log2 ratio)
-2
-1
0
1 at 1552.440 kb on + strand, within MIT1002_01403 at 1553.220 kb on + strand at 1553.220 kb on + strand at 1553.220 kb on + strand at 1553.220 kb on + strand at 1553.220 kb on + strand at 1553.228 kb on - strand at 1553.228 kb on - strand at 1553.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction monoculture; Experiment A, time point 1 remove 1,552,440 + MIT1002_01403 0.12 -1.8 1,553,220 + +0.5 1,553,220 + -0.4 1,553,220 + -0.2 1,553,220 + +0.6 1,553,220 + -0.7 1,553,228 - +0.8 1,553,228 - +1.3 1,553,228 - -0.4
Or see this region's nucleotide sequence