Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pdeB and ybaA are separated by 41 nucleotides ybaA and atl are separated by 378 nucleotides atl and ybaY are separated by 30 nucleotides ybaY and tesB are separated by 217 nucleotides
BNILDI_22580: pdeB - cyclic-guanylate-specific phosphodiesterase PdeB, at 4,544,090 to 4,545,640
pdeB
BNILDI_22585: ybaA - Uncharacterized protein YbaA, at 4,545,682 to 4,546,035
ybaA
BNILDI_22595: atl - DNA base-flipping protein, at 4,546,414 to 4,546,725
atl
BNILDI_22600: ybaY - Uncharacterized lipoprotein YbaY, at 4,546,756 to 4,547,328
ybaY
BNILDI_22605: tesB - acyl-CoA thioesterase II, at 4,547,546 to 4,548,406
tesB
Position (kb)
4546
4547 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4545.469 kb on + strand, within pdeB at 4545.530 kb on - strand at 4545.584 kb on + strand at 4545.615 kb on + strand at 4545.683 kb on + strand at 4545.714 kb on - strand at 4545.832 kb on - strand, within ybaA at 4546.028 kb on + strand at 4546.028 kb on + strand at 4546.028 kb on + strand at 4546.029 kb on - strand at 4546.029 kb on - strand at 4546.042 kb on + strand at 4546.042 kb on + strand at 4546.043 kb on - strand at 4546.384 kb on + strand at 4546.384 kb on + strand at 4546.477 kb on + strand, within atl at 4546.477 kb on + strand, within atl at 4546.489 kb on + strand, within atl at 4546.490 kb on - strand, within atl at 4546.550 kb on + strand, within atl at 4546.720 kb on + strand at 4546.724 kb on - strand at 4546.724 kb on - strand at 4546.761 kb on - strand at 4546.768 kb on - strand at 4546.819 kb on + strand, within ybaY at 4546.819 kb on + strand, within ybaY at 4546.824 kb on + strand, within ybaY at 4546.896 kb on + strand, within ybaY at 4546.938 kb on + strand, within ybaY at 4546.939 kb on - strand, within ybaY at 4546.943 kb on - strand, within ybaY at 4546.984 kb on - strand, within ybaY at 4547.047 kb on - strand, within ybaY at 4547.055 kb on + strand, within ybaY at 4547.220 kb on + strand, within ybaY at 4547.221 kb on - strand, within ybaY at 4547.221 kb on - strand, within ybaY at 4547.221 kb on - strand, within ybaY at 4547.226 kb on + strand, within ybaY at 4547.227 kb on - strand, within ybaY at 4547.340 kb on + strand at 4547.343 kb on + strand at 4547.497 kb on - strand at 4547.543 kb on + strand at 4547.546 kb on - strand at 4547.631 kb on + strand at 4547.678 kb on + strand, within tesB at 4547.678 kb on + strand, within tesB at 4547.679 kb on - strand, within tesB at 4547.679 kb on - strand, within tesB
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 4,545,469 + pdeB BNILDI_22580 0.89 +0.9 4,545,530 - -0.0 4,545,584 + +0.3 4,545,615 + -0.5 4,545,683 + -0.6 4,545,714 - -2.2 4,545,832 - ybaA BNILDI_22585 0.42 -0.8 4,546,028 + -1.7 4,546,028 + +1.7 4,546,028 + -0.6 4,546,029 - +1.3 4,546,029 - -1.7 4,546,042 + +0.4 4,546,042 + -0.8 4,546,043 - -0.8 4,546,384 + -0.4 4,546,384 + -0.9 4,546,477 + atl BNILDI_22595 0.20 -0.7 4,546,477 + atl BNILDI_22595 0.20 -1.3 4,546,489 + atl BNILDI_22595 0.24 -0.9 4,546,490 - atl BNILDI_22595 0.24 -0.7 4,546,550 + atl BNILDI_22595 0.44 -1.2 4,546,720 + +0.4 4,546,724 - -0.2 4,546,724 - -0.3 4,546,761 - -0.9 4,546,768 - -1.1 4,546,819 + ybaY BNILDI_22600 0.11 -0.8 4,546,819 + ybaY BNILDI_22600 0.11 +0.9 4,546,824 + ybaY BNILDI_22600 0.12 +0.1 4,546,896 + ybaY BNILDI_22600 0.24 +1.1 4,546,938 + ybaY BNILDI_22600 0.32 +1.1 4,546,939 - ybaY BNILDI_22600 0.32 +0.2 4,546,943 - ybaY BNILDI_22600 0.33 +1.9 4,546,984 - ybaY BNILDI_22600 0.40 -1.7 4,547,047 - ybaY BNILDI_22600 0.51 -0.6 4,547,055 + ybaY BNILDI_22600 0.52 -0.3 4,547,220 + ybaY BNILDI_22600 0.81 -1.1 4,547,221 - ybaY BNILDI_22600 0.81 -0.3 4,547,221 - ybaY BNILDI_22600 0.81 -0.2 4,547,221 - ybaY BNILDI_22600 0.81 +0.9 4,547,226 + ybaY BNILDI_22600 0.82 -1.5 4,547,227 - ybaY BNILDI_22600 0.82 -3.2 4,547,340 + -0.8 4,547,343 + +0.3 4,547,497 - +1.5 4,547,543 + -0.7 4,547,546 - -1.7 4,547,631 + -0.8 4,547,678 + tesB BNILDI_22605 0.15 +0.1 4,547,678 + tesB BNILDI_22605 0.15 -0.1 4,547,679 - tesB BNILDI_22605 0.15 -2.9 4,547,679 - tesB BNILDI_22605 0.15 -0.5
Or see this region's nucleotide sequence