Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycaQ and lpxK are separated by 36 nucleotides lpxK and msbA overlap by 4 nucleotides
BNILDI_20260: ycaQ - Uncharacterized protein YcaQ, at 4,071,053 to 4,072,285
ycaQ
BNILDI_20265: lpxK - tetraacyldisaccharide 4'-kinase, at 4,072,322 to 4,073,308
lpxK
BNILDI_20270: msbA - lipid A ABC transporter ATP-binding protein/permease MsbA, at 4,073,305 to 4,075,053
msbA
Position (kb)
4072
4073
4074 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4071.344 kb on - strand, within ycaQ at 4071.345 kb on + strand, within ycaQ at 4071.346 kb on - strand, within ycaQ at 4071.623 kb on + strand, within ycaQ at 4071.725 kb on + strand, within ycaQ at 4071.726 kb on - strand, within ycaQ at 4071.732 kb on + strand, within ycaQ at 4071.786 kb on - strand, within ycaQ at 4071.786 kb on - strand, within ycaQ at 4071.786 kb on - strand, within ycaQ at 4071.880 kb on + strand, within ycaQ at 4071.889 kb on + strand, within ycaQ at 4071.890 kb on - strand, within ycaQ at 4071.890 kb on - strand, within ycaQ at 4071.890 kb on - strand, within ycaQ at 4071.890 kb on - strand, within ycaQ at 4071.945 kb on + strand, within ycaQ at 4072.012 kb on + strand, within ycaQ at 4072.117 kb on - strand, within ycaQ at 4072.124 kb on - strand, within ycaQ at 4072.136 kb on - strand, within ycaQ at 4072.136 kb on - strand, within ycaQ at 4072.323 kb on + strand at 4072.323 kb on + strand at 4072.323 kb on + strand at 4072.323 kb on + strand at 4072.323 kb on + strand at 4072.324 kb on - strand at 4072.324 kb on - strand at 4072.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 4,071,344 - ycaQ BNILDI_20260 0.24 +0.4 4,071,345 + ycaQ BNILDI_20260 0.24 +0.5 4,071,346 - ycaQ BNILDI_20260 0.24 +0.2 4,071,623 + ycaQ BNILDI_20260 0.46 +1.9 4,071,725 + ycaQ BNILDI_20260 0.55 +0.5 4,071,726 - ycaQ BNILDI_20260 0.55 -1.2 4,071,732 + ycaQ BNILDI_20260 0.55 -1.0 4,071,786 - ycaQ BNILDI_20260 0.59 +1.1 4,071,786 - ycaQ BNILDI_20260 0.59 +1.3 4,071,786 - ycaQ BNILDI_20260 0.59 -0.1 4,071,880 + ycaQ BNILDI_20260 0.67 -2.2 4,071,889 + ycaQ BNILDI_20260 0.68 +1.9 4,071,890 - ycaQ BNILDI_20260 0.68 +0.2 4,071,890 - ycaQ BNILDI_20260 0.68 -0.1 4,071,890 - ycaQ BNILDI_20260 0.68 +0.4 4,071,890 - ycaQ BNILDI_20260 0.68 +0.5 4,071,945 + ycaQ BNILDI_20260 0.72 -0.1 4,072,012 + ycaQ BNILDI_20260 0.78 +0.1 4,072,117 - ycaQ BNILDI_20260 0.86 +0.3 4,072,124 - ycaQ BNILDI_20260 0.87 +0.5 4,072,136 - ycaQ BNILDI_20260 0.88 +0.3 4,072,136 - ycaQ BNILDI_20260 0.88 +3.3 4,072,323 + -1.3 4,072,323 + +1.3 4,072,323 + +1.8 4,072,323 + -1.0 4,072,323 + -0.9 4,072,324 - -0.2 4,072,324 - +1.8 4,072,347 - -0.7
Or see this region's nucleotide sequence