Strain Fitness in Escherichia coli ECRC62 around BNILDI_20265

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycaQ and lpxK are separated by 36 nucleotideslpxK and msbA overlap by 4 nucleotides BNILDI_20260: ycaQ - Uncharacterized protein YcaQ, at 4,071,053 to 4,072,285 ycaQ BNILDI_20265: lpxK - tetraacyldisaccharide 4'-kinase, at 4,072,322 to 4,073,308 lpxK BNILDI_20270: msbA - lipid A ABC transporter ATP-binding protein/permease MsbA, at 4,073,305 to 4,075,053 msbA Position (kb) 4072 4073 4074Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4071.344 kb on - strand, within ycaQat 4071.345 kb on + strand, within ycaQat 4071.346 kb on - strand, within ycaQat 4071.623 kb on + strand, within ycaQat 4071.725 kb on + strand, within ycaQat 4071.726 kb on - strand, within ycaQat 4071.732 kb on + strand, within ycaQat 4071.786 kb on - strand, within ycaQat 4071.786 kb on - strand, within ycaQat 4071.786 kb on - strand, within ycaQat 4071.880 kb on + strand, within ycaQat 4071.889 kb on + strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.890 kb on - strand, within ycaQat 4071.945 kb on + strand, within ycaQat 4072.012 kb on + strand, within ycaQat 4072.117 kb on - strand, within ycaQat 4072.124 kb on - strand, within ycaQat 4072.136 kb on - strand, within ycaQat 4072.136 kb on - strand, within ycaQat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.323 kb on + strandat 4072.324 kb on - strandat 4072.324 kb on - strandat 4072.347 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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4,071,344 - ycaQ BNILDI_20260 0.24 +0.4
4,071,345 + ycaQ BNILDI_20260 0.24 +0.5
4,071,346 - ycaQ BNILDI_20260 0.24 +0.2
4,071,623 + ycaQ BNILDI_20260 0.46 +1.9
4,071,725 + ycaQ BNILDI_20260 0.55 +0.5
4,071,726 - ycaQ BNILDI_20260 0.55 -1.2
4,071,732 + ycaQ BNILDI_20260 0.55 -1.0
4,071,786 - ycaQ BNILDI_20260 0.59 +1.1
4,071,786 - ycaQ BNILDI_20260 0.59 +1.3
4,071,786 - ycaQ BNILDI_20260 0.59 -0.1
4,071,880 + ycaQ BNILDI_20260 0.67 -2.2
4,071,889 + ycaQ BNILDI_20260 0.68 +1.9
4,071,890 - ycaQ BNILDI_20260 0.68 +0.2
4,071,890 - ycaQ BNILDI_20260 0.68 -0.1
4,071,890 - ycaQ BNILDI_20260 0.68 +0.4
4,071,890 - ycaQ BNILDI_20260 0.68 +0.5
4,071,945 + ycaQ BNILDI_20260 0.72 -0.1
4,072,012 + ycaQ BNILDI_20260 0.78 +0.1
4,072,117 - ycaQ BNILDI_20260 0.86 +0.3
4,072,124 - ycaQ BNILDI_20260 0.87 +0.5
4,072,136 - ycaQ BNILDI_20260 0.88 +0.3
4,072,136 - ycaQ BNILDI_20260 0.88 +3.3
4,072,323 + -1.3
4,072,323 + +1.3
4,072,323 + +1.8
4,072,323 + -1.0
4,072,323 + -0.9
4,072,324 - -0.2
4,072,324 - +1.8
4,072,347 - -0.7

Or see this region's nucleotide sequence