Strain Fitness in Escherichia coli ECRC62 around BNILDI_17275

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyneE and tam are separated by 3 nucleotidestam and lsrG are separated by 56 nucleotideslsrG and lsrF are separated by 23 nucleotideslsrF and lsrB are separated by 26 nucleotides BNILDI_17265: yneE - UPF0187 protein YneE, at 3,458,588 to 3,459,502 yneE BNILDI_17270: tam - trans-aconitate 2-methyltransferase, at 3,459,506 to 3,460,264 tam BNILDI_17275: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 3,460,321 to 3,460,611 lsrG BNILDI_17280: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 3,460,635 to 3,461,510 lsrF BNILDI_17285: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 3,461,537 to 3,462,559 lsrB Position (kb) 3460 3461Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3459.554 kb on + strandat 3459.604 kb on - strand, within tamat 3459.610 kb on + strand, within tamat 3459.715 kb on - strand, within tamat 3459.717 kb on + strand, within tamat 3459.718 kb on - strand, within tamat 3459.764 kb on - strand, within tamat 3459.768 kb on + strand, within tamat 3459.772 kb on - strand, within tamat 3459.905 kb on - strand, within tamat 3460.164 kb on + strand, within tamat 3460.239 kb on + strandat 3460.239 kb on + strandat 3460.241 kb on + strandat 3460.242 kb on - strandat 3460.270 kb on + strandat 3460.271 kb on - strandat 3460.299 kb on - strandat 3460.444 kb on - strand, within lsrGat 3460.458 kb on + strand, within lsrGat 3460.461 kb on - strand, within lsrGat 3460.611 kb on + strandat 3460.612 kb on - strandat 3460.655 kb on - strandat 3460.746 kb on - strand, within lsrFat 3460.895 kb on - strand, within lsrFat 3460.897 kb on + strand, within lsrFat 3461.084 kb on - strand, within lsrFat 3461.259 kb on + strand, within lsrFat 3461.259 kb on + strand, within lsrFat 3461.294 kb on + strand, within lsrFat 3461.294 kb on + strand, within lsrFat 3461.333 kb on - strand, within lsrFat 3461.500 kb on - strandat 3461.525 kb on - strandat 3461.595 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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3,459,554 + -0.6
3,459,604 - tam BNILDI_17270 0.13 +0.3
3,459,610 + tam BNILDI_17270 0.14 +2.8
3,459,715 - tam BNILDI_17270 0.28 +0.1
3,459,717 + tam BNILDI_17270 0.28 -1.0
3,459,718 - tam BNILDI_17270 0.28 +0.1
3,459,764 - tam BNILDI_17270 0.34 +0.2
3,459,768 + tam BNILDI_17270 0.35 +1.2
3,459,772 - tam BNILDI_17270 0.35 -0.4
3,459,905 - tam BNILDI_17270 0.53 +2.8
3,460,164 + tam BNILDI_17270 0.87 +0.4
3,460,239 + -2.1
3,460,239 + -0.2
3,460,241 + -1.7
3,460,242 - +0.3
3,460,270 + -0.4
3,460,271 - +0.1
3,460,299 - -0.1
3,460,444 - lsrG BNILDI_17275 0.42 +1.2
3,460,458 + lsrG BNILDI_17275 0.47 -0.9
3,460,461 - lsrG BNILDI_17275 0.48 -0.0
3,460,611 + +1.0
3,460,612 - +2.8
3,460,655 - +0.3
3,460,746 - lsrF BNILDI_17280 0.13 -2.5
3,460,895 - lsrF BNILDI_17280 0.30 -0.9
3,460,897 + lsrF BNILDI_17280 0.30 +1.0
3,461,084 - lsrF BNILDI_17280 0.51 -1.7
3,461,259 + lsrF BNILDI_17280 0.71 -0.5
3,461,259 + lsrF BNILDI_17280 0.71 -0.4
3,461,294 + lsrF BNILDI_17280 0.75 -1.3
3,461,294 + lsrF BNILDI_17280 0.75 +2.8
3,461,333 - lsrF BNILDI_17280 0.80 +0.6
3,461,500 - -0.0
3,461,525 - +0.3
3,461,595 - -0.8

Or see this region's nucleotide sequence