Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutY and trmB are separated by 160 nucleotides trmB and yggL overlap by 1 nucleotides yggL and yggN are separated by 183 nucleotides
BNILDI_09645: mutY - A/G-specific adenine glycosylase, at 1,943,457 to 1,944,509
mutY
BNILDI_09650: trmB - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 1,944,670 to 1,945,389
trmB
BNILDI_09655: yggL - Uncharacterized protein YggL, at 1,945,389 to 1,945,715
yggL
BNILDI_09660: yggN - Uncharacterized protein YggN, at 1,945,899 to 1,946,618
yggN
Position (kb)
1945
1946 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1944.460 kb on + strand at 1944.461 kb on - strand at 1944.617 kb on + strand at 1944.618 kb on - strand at 1944.618 kb on - strand at 1944.618 kb on - strand at 1944.661 kb on + strand at 1944.733 kb on - strand at 1944.790 kb on + strand, within trmB at 1944.825 kb on + strand, within trmB at 1944.826 kb on - strand, within trmB at 1944.826 kb on - strand, within trmB at 1944.826 kb on - strand, within trmB at 1945.004 kb on + strand, within trmB at 1945.050 kb on + strand, within trmB at 1945.051 kb on - strand, within trmB at 1945.123 kb on - strand, within trmB at 1945.163 kb on + strand, within trmB at 1945.220 kb on - strand, within trmB at 1945.230 kb on + strand, within trmB at 1945.230 kb on + strand, within trmB at 1945.230 kb on + strand, within trmB at 1945.231 kb on - strand, within trmB at 1945.231 kb on - strand, within trmB at 1945.263 kb on + strand, within trmB at 1945.288 kb on + strand, within trmB at 1945.367 kb on + strand at 1945.367 kb on + strand at 1945.368 kb on - strand at 1945.368 kb on - strand at 1945.387 kb on + strand at 1945.388 kb on - strand at 1945.388 kb on - strand at 1945.505 kb on + strand, within yggL at 1945.505 kb on + strand, within yggL at 1945.523 kb on + strand, within yggL at 1945.523 kb on + strand, within yggL at 1945.524 kb on - strand, within yggL at 1945.524 kb on - strand, within yggL at 1945.647 kb on - strand, within yggL at 1945.678 kb on + strand, within yggL at 1945.714 kb on - strand at 1945.723 kb on + strand at 1945.766 kb on - strand at 1945.812 kb on + strand at 1945.813 kb on - strand at 1945.898 kb on + strand at 1945.898 kb on + strand at 1945.898 kb on + strand at 1945.898 kb on + strand at 1945.898 kb on + strand at 1945.899 kb on - strand at 1945.899 kb on - strand at 1946.070 kb on + strand, within yggN at 1946.070 kb on + strand, within yggN at 1946.070 kb on + strand, within yggN at 1946.071 kb on - strand, within yggN at 1946.086 kb on - strand, within yggN at 1946.124 kb on + strand, within yggN at 1946.137 kb on + strand, within yggN at 1946.138 kb on - strand, within yggN at 1946.138 kb on - strand, within yggN at 1946.141 kb on + strand, within yggN at 1946.141 kb on + strand, within yggN at 1946.142 kb on - strand, within yggN at 1946.195 kb on + strand, within yggN at 1946.213 kb on + strand, within yggN at 1946.334 kb on + strand, within yggN at 1946.334 kb on + strand, within yggN at 1946.336 kb on + strand, within yggN at 1946.337 kb on - strand, within yggN at 1946.461 kb on + strand, within yggN at 1946.561 kb on + strand at 1946.616 kb on + strand at 1946.630 kb on - strand at 1946.640 kb on - strand at 1946.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 1,944,460 + +1.9 1,944,461 - +0.5 1,944,617 + -1.4 1,944,618 - +0.4 1,944,618 - +3.5 1,944,618 - +0.2 1,944,661 + -1.0 1,944,733 - -1.5 1,944,790 + trmB BNILDI_09650 0.17 -1.5 1,944,825 + trmB BNILDI_09650 0.22 +0.5 1,944,826 - trmB BNILDI_09650 0.22 -0.3 1,944,826 - trmB BNILDI_09650 0.22 -0.2 1,944,826 - trmB BNILDI_09650 0.22 +0.5 1,945,004 + trmB BNILDI_09650 0.46 +0.8 1,945,050 + trmB BNILDI_09650 0.53 -0.5 1,945,051 - trmB BNILDI_09650 0.53 +0.0 1,945,123 - trmB BNILDI_09650 0.63 -0.1 1,945,163 + trmB BNILDI_09650 0.68 -0.2 1,945,220 - trmB BNILDI_09650 0.76 +0.1 1,945,230 + trmB BNILDI_09650 0.78 +0.4 1,945,230 + trmB BNILDI_09650 0.78 -0.1 1,945,230 + trmB BNILDI_09650 0.78 +0.5 1,945,231 - trmB BNILDI_09650 0.78 +0.1 1,945,231 - trmB BNILDI_09650 0.78 +0.5 1,945,263 + trmB BNILDI_09650 0.82 +0.4 1,945,288 + trmB BNILDI_09650 0.86 -3.3 1,945,367 + -0.4 1,945,367 + -1.5 1,945,368 - +1.5 1,945,368 - -1.4 1,945,387 + +0.5 1,945,388 - -1.1 1,945,388 - +0.1 1,945,505 + yggL BNILDI_09655 0.35 -0.4 1,945,505 + yggL BNILDI_09655 0.35 -0.2 1,945,523 + yggL BNILDI_09655 0.41 -0.0 1,945,523 + yggL BNILDI_09655 0.41 +0.4 1,945,524 - yggL BNILDI_09655 0.41 +0.7 1,945,524 - yggL BNILDI_09655 0.41 +0.6 1,945,647 - yggL BNILDI_09655 0.79 +0.7 1,945,678 + yggL BNILDI_09655 0.88 -0.7 1,945,714 - -1.2 1,945,723 + +0.1 1,945,766 - +0.9 1,945,812 + +0.7 1,945,813 - +1.5 1,945,898 + +0.5 1,945,898 + -0.9 1,945,898 + +0.3 1,945,898 + -0.7 1,945,898 + -0.6 1,945,899 - +1.3 1,945,899 - -1.3 1,946,070 + yggN BNILDI_09660 0.24 +0.9 1,946,070 + yggN BNILDI_09660 0.24 -0.7 1,946,070 + yggN BNILDI_09660 0.24 +0.5 1,946,071 - yggN BNILDI_09660 0.24 +1.5 1,946,086 - yggN BNILDI_09660 0.26 +0.3 1,946,124 + yggN BNILDI_09660 0.31 -0.9 1,946,137 + yggN BNILDI_09660 0.33 -1.0 1,946,138 - yggN BNILDI_09660 0.33 +2.5 1,946,138 - yggN BNILDI_09660 0.33 -0.6 1,946,141 + yggN BNILDI_09660 0.34 +0.9 1,946,141 + yggN BNILDI_09660 0.34 -0.1 1,946,142 - yggN BNILDI_09660 0.34 +0.7 1,946,195 + yggN BNILDI_09660 0.41 -1.4 1,946,213 + yggN BNILDI_09660 0.44 +0.1 1,946,334 + yggN BNILDI_09660 0.60 -0.6 1,946,334 + yggN BNILDI_09660 0.60 -0.5 1,946,336 + yggN BNILDI_09660 0.61 -0.7 1,946,337 - yggN BNILDI_09660 0.61 +2.9 1,946,461 + yggN BNILDI_09660 0.78 -0.7 1,946,561 + -0.2 1,946,616 + +0.5 1,946,630 - -0.2 1,946,640 - +0.9 1,946,694 - +0.9
Or see this region's nucleotide sequence