Strain Fitness in Escherichia coli ECRC62 around BNILDI_05065

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpldB and yigL are separated by 7 nucleotidesyigL and bioP are separated by 75 nucleotidesbioP and metR overlap by 113 nucleotidesmetR and metE are separated by 116 nucleotides BNILDI_05055: pldB - lysophospholipase L2, at 1,001,303 to 1,002,325 pldB BNILDI_05060: yigL - sugar/pyridoxal phosphate phosphatase YigL, at 1,002,333 to 1,003,133 yigL BNILDI_05065: bioP - biotin transporter, at 1,003,209 to 1,004,108 bioP BNILDI_05070: metR - HTH-type transcriptional regulator MetR, at 1,003,996 to 1,004,949 metR BNILDI_05075: metE - 5-methyltetrahydropteroyltriglutamate--homocystei ne S-methyltransferase, at 1,005,066 to 1,007,327 metE Position (kb) 1003 1004 1005Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1002.212 kb on + strand, within pldBat 1002.213 kb on - strand, within pldBat 1002.337 kb on - strandat 1002.337 kb on - strandat 1002.371 kb on - strandat 1002.386 kb on + strandat 1002.386 kb on + strandat 1002.386 kb on + strandat 1002.386 kb on + strandat 1002.387 kb on - strandat 1002.387 kb on - strandat 1002.506 kb on + strand, within yigLat 1002.513 kb on + strand, within yigLat 1002.513 kb on + strand, within yigLat 1002.514 kb on - strand, within yigLat 1002.514 kb on - strand, within yigLat 1002.514 kb on - strand, within yigLat 1002.514 kb on - strand, within yigLat 1002.575 kb on + strand, within yigLat 1002.576 kb on - strand, within yigLat 1002.613 kb on + strand, within yigLat 1002.614 kb on - strand, within yigLat 1002.638 kb on + strand, within yigLat 1002.638 kb on + strand, within yigLat 1002.733 kb on - strand, within yigLat 1002.745 kb on + strand, within yigLat 1002.959 kb on + strand, within yigLat 1003.079 kb on + strandat 1003.079 kb on + strandat 1003.122 kb on + strandat 1003.122 kb on + strandat 1003.122 kb on + strandat 1003.122 kb on + strandat 1003.123 kb on - strandat 1003.123 kb on - strandat 1003.123 kb on - strandat 1003.216 kb on + strandat 1003.216 kb on + strandat 1003.217 kb on - strandat 1003.217 kb on - strandat 1003.220 kb on + strandat 1003.220 kb on + strandat 1003.221 kb on - strandat 1003.221 kb on - strandat 1003.261 kb on - strandat 1003.269 kb on + strandat 1003.269 kb on + strandat 1003.289 kb on + strandat 1003.293 kb on + strandat 1003.293 kb on + strandat 1003.294 kb on - strandat 1003.294 kb on - strandat 1003.353 kb on - strand, within bioPat 1003.365 kb on - strand, within bioPat 1003.386 kb on + strand, within bioPat 1003.386 kb on + strand, within bioPat 1003.387 kb on - strand, within bioPat 1003.387 kb on - strand, within bioPat 1003.387 kb on - strand, within bioPat 1003.388 kb on + strand, within bioPat 1003.388 kb on + strand, within bioPat 1003.389 kb on - strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.422 kb on + strand, within bioPat 1003.423 kb on - strand, within bioPat 1003.423 kb on - strand, within bioPat 1003.423 kb on - strand, within bioPat 1003.424 kb on + strand, within bioPat 1003.425 kb on - strand, within bioPat 1003.425 kb on - strand, within bioPat 1003.425 kb on - strand, within bioPat 1003.425 kb on - strand, within bioPat 1003.425 kb on - strand, within bioPat 1003.444 kb on - strand, within bioPat 1003.450 kb on - strand, within bioPat 1003.497 kb on + strand, within bioPat 1003.498 kb on - strand, within bioPat 1003.498 kb on - strand, within bioPat 1003.512 kb on + strand, within bioPat 1003.512 kb on + strand, within bioPat 1003.653 kb on - strand, within bioPat 1003.680 kb on + strand, within bioPat 1003.719 kb on - strand, within bioPat 1003.738 kb on - strand, within bioPat 1003.868 kb on + strand, within bioPat 1003.868 kb on + strand, within bioPat 1003.916 kb on + strand, within bioPat 1003.917 kb on - strand, within bioPat 1003.917 kb on - strand, within bioPat 1003.925 kb on + strand, within bioPat 1003.925 kb on + strand, within bioPat 1004.039 kb on + strandat 1004.039 kb on + strandat 1004.040 kb on - strandat 1004.040 kb on - strandat 1004.040 kb on - strandat 1004.118 kb on + strand, within metRat 1004.224 kb on + strand, within metRat 1004.224 kb on + strand, within metRat 1004.228 kb on - strand, within metRat 1004.284 kb on + strand, within metRat 1004.284 kb on + strand, within metRat 1004.285 kb on - strand, within metRat 1004.285 kb on - strand, within metRat 1004.597 kb on - strand, within metRat 1004.649 kb on + strand, within metRat 1004.656 kb on - strand, within metRat 1004.797 kb on - strand, within metRat 1004.806 kb on + strand, within metRat 1004.920 kb on + strandat 1004.938 kb on + strandat 1004.939 kb on - strandat 1004.993 kb on - strandat 1004.993 kb on - strandat 1004.994 kb on + strandat 1004.994 kb on + strandat 1004.995 kb on - strandat 1004.995 kb on - strandat 1005.062 kb on + strandat 1005.062 kb on + strandat 1005.062 kb on + strandat 1005.063 kb on - strandat 1005.063 kb on - strandat 1005.065 kb on - strandat 1005.065 kb on - strandat 1005.065 kb on - strandat 1005.065 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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1,002,212 + pldB BNILDI_05055 0.89 -1.5
1,002,213 - pldB BNILDI_05055 0.89 -0.1
1,002,337 - -0.2
1,002,337 - -2.3
1,002,371 - +0.2
1,002,386 + -0.4
1,002,386 + +3.1
1,002,386 + -2.4
1,002,386 + -1.3
1,002,387 - -1.8
1,002,387 - -0.7
1,002,506 + yigL BNILDI_05060 0.22 -1.4
1,002,513 + yigL BNILDI_05060 0.22 -1.2
1,002,513 + yigL BNILDI_05060 0.22 -1.3
1,002,514 - yigL BNILDI_05060 0.23 -1.5
1,002,514 - yigL BNILDI_05060 0.23 +1.1
1,002,514 - yigL BNILDI_05060 0.23 +0.8
1,002,514 - yigL BNILDI_05060 0.23 +0.7
1,002,575 + yigL BNILDI_05060 0.30 -0.7
1,002,576 - yigL BNILDI_05060 0.30 +1.3
1,002,613 + yigL BNILDI_05060 0.35 -1.3
1,002,614 - yigL BNILDI_05060 0.35 -0.7
1,002,638 + yigL BNILDI_05060 0.38 -0.6
1,002,638 + yigL BNILDI_05060 0.38 -1.1
1,002,733 - yigL BNILDI_05060 0.50 -1.5
1,002,745 + yigL BNILDI_05060 0.51 -1.0
1,002,959 + yigL BNILDI_05060 0.78 +0.6
1,003,079 + -0.3
1,003,079 + -1.1
1,003,122 + -1.5
1,003,122 + +1.6
1,003,122 + +1.4
1,003,122 + -0.0
1,003,123 - -1.6
1,003,123 - +0.3
1,003,123 - -0.7
1,003,216 + +0.7
1,003,216 + -0.1
1,003,217 - -2.4
1,003,217 - +0.1
1,003,220 + -0.4
1,003,220 + +0.8
1,003,221 - -1.0
1,003,221 - -0.0
1,003,261 - +0.2
1,003,269 + -1.0
1,003,269 + +0.9
1,003,289 + -1.1
1,003,293 + -0.8
1,003,293 + -0.5
1,003,294 - -0.7
1,003,294 - -0.3
1,003,353 - bioP BNILDI_05065 0.16 -0.3
1,003,365 - bioP BNILDI_05065 0.17 -1.2
1,003,386 + bioP BNILDI_05065 0.20 +0.8
1,003,386 + bioP BNILDI_05065 0.20 -0.2
1,003,387 - bioP BNILDI_05065 0.20 +0.3
1,003,387 - bioP BNILDI_05065 0.20 +1.7
1,003,387 - bioP BNILDI_05065 0.20 -1.9
1,003,388 + bioP BNILDI_05065 0.20 +0.2
1,003,388 + bioP BNILDI_05065 0.20 -0.7
1,003,389 - bioP BNILDI_05065 0.20 +0.1
1,003,422 + bioP BNILDI_05065 0.24 -0.6
1,003,422 + bioP BNILDI_05065 0.24 -2.2
1,003,422 + bioP BNILDI_05065 0.24 -0.8
1,003,422 + bioP BNILDI_05065 0.24 -2.2
1,003,422 + bioP BNILDI_05065 0.24 +0.9
1,003,422 + bioP BNILDI_05065 0.24 -0.8
1,003,422 + bioP BNILDI_05065 0.24 -1.4
1,003,422 + bioP BNILDI_05065 0.24 +0.7
1,003,422 + bioP BNILDI_05065 0.24 +0.3
1,003,423 - bioP BNILDI_05065 0.24 -1.6
1,003,423 - bioP BNILDI_05065 0.24 -0.2
1,003,423 - bioP BNILDI_05065 0.24 +0.7
1,003,424 + bioP BNILDI_05065 0.24 -0.7
1,003,425 - bioP BNILDI_05065 0.24 -0.1
1,003,425 - bioP BNILDI_05065 0.24 -1.2
1,003,425 - bioP BNILDI_05065 0.24 +2.3
1,003,425 - bioP BNILDI_05065 0.24 +0.6
1,003,425 - bioP BNILDI_05065 0.24 -0.4
1,003,444 - bioP BNILDI_05065 0.26 -1.3
1,003,450 - bioP BNILDI_05065 0.27 +1.3
1,003,497 + bioP BNILDI_05065 0.32 -0.2
1,003,498 - bioP BNILDI_05065 0.32 -0.3
1,003,498 - bioP BNILDI_05065 0.32 -2.4
1,003,512 + bioP BNILDI_05065 0.34 -0.3
1,003,512 + bioP BNILDI_05065 0.34 -1.1
1,003,653 - bioP BNILDI_05065 0.49 +0.7
1,003,680 + bioP BNILDI_05065 0.52 +2.5
1,003,719 - bioP BNILDI_05065 0.57 -0.4
1,003,738 - bioP BNILDI_05065 0.59 -0.5
1,003,868 + bioP BNILDI_05065 0.73 -3.0
1,003,868 + bioP BNILDI_05065 0.73 -1.0
1,003,916 + bioP BNILDI_05065 0.79 +0.1
1,003,917 - bioP BNILDI_05065 0.79 -0.5
1,003,917 - bioP BNILDI_05065 0.79 -1.9
1,003,925 + bioP BNILDI_05065 0.80 -0.7
1,003,925 + bioP BNILDI_05065 0.80 +1.3
1,004,039 + +0.7
1,004,039 + -0.2
1,004,040 - +0.3
1,004,040 - +0.4
1,004,040 - -1.4
1,004,118 + metR BNILDI_05070 0.13 -0.4
1,004,224 + metR BNILDI_05070 0.24 -0.5
1,004,224 + metR BNILDI_05070 0.24 -0.6
1,004,228 - metR BNILDI_05070 0.24 -0.5
1,004,284 + metR BNILDI_05070 0.30 -1.0
1,004,284 + metR BNILDI_05070 0.30 +0.2
1,004,285 - metR BNILDI_05070 0.30 +1.4
1,004,285 - metR BNILDI_05070 0.30 -0.2
1,004,597 - metR BNILDI_05070 0.63 -0.4
1,004,649 + metR BNILDI_05070 0.68 -1.3
1,004,656 - metR BNILDI_05070 0.69 -1.3
1,004,797 - metR BNILDI_05070 0.84 -0.4
1,004,806 + metR BNILDI_05070 0.85 -0.3
1,004,920 + -0.6
1,004,938 + +2.3
1,004,939 - +0.3
1,004,993 - +1.3
1,004,993 - -0.6
1,004,994 + -0.3
1,004,994 + -0.1
1,004,995 - -0.4
1,004,995 - -0.8
1,005,062 + -0.5
1,005,062 + -1.5
1,005,062 + -0.4
1,005,063 - -0.4
1,005,063 - -1.2
1,005,065 - +1.4
1,005,065 - -0.0
1,005,065 - -1.0
1,005,065 - -0.4

Or see this region's nucleotide sequence