Strain Fitness in Escherichia coli ECRC62 around BNILDI_02110

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhdY and yhdZ are separated by 7 nucleotidesyhdZ and 5S_rrna are separated by 230 nucleotides5S_rrna and Thr_trna are separated by 39 nucleotidesThr_trna and 5S_rrna are separated by 14 nucleotides5S_rrna and 23S_rrna are separated by 95 nucleotides BNILDI_02105: yhdY - Inner membrane amino-acid ABC transporter permease protein YhdY, at 418,393 to 419,496 yhdY BNILDI_02110: yhdZ - Uncharacterized amino-acid ABC transporter ATP-binding protein YhdZ, at 419,504 to 420,262 yhdZ BNILDI_02115: 5S_rrna - 5S ribosomal RNA, at 420,493 to 420,608 _rrna BNILDI_02120: Thr_trna - tRNA-Thr(ggt), at 420,648 to 420,723 _trna BNILDI_02125: 5S_rrna - 5S ribosomal RNA, at 420,738 to 420,853 _rrna BNILDI_02130: 23S_rrna - 23S ribosomal RNA, at 420,949 to 423,852 _rrna Position (kb) 419 420 421Strain fitness (log2 ratio) -2 -1 0 1 2at 418.653 kb on + strand, within yhdYat 418.653 kb on + strand, within yhdYat 418.654 kb on - strand, within yhdYat 418.654 kb on - strand, within yhdYat 418.658 kb on - strand, within yhdYat 418.692 kb on + strand, within yhdYat 418.765 kb on + strand, within yhdYat 418.767 kb on + strand, within yhdYat 418.768 kb on - strand, within yhdYat 418.817 kb on - strand, within yhdYat 418.817 kb on - strand, within yhdYat 418.875 kb on - strand, within yhdYat 418.884 kb on + strand, within yhdYat 418.884 kb on + strand, within yhdYat 418.968 kb on + strand, within yhdYat 419.022 kb on - strand, within yhdYat 419.029 kb on - strand, within yhdYat 419.029 kb on - strand, within yhdYat 419.125 kb on + strand, within yhdYat 419.125 kb on + strand, within yhdYat 419.126 kb on - strand, within yhdYat 419.126 kb on - strand, within yhdYat 419.126 kb on - strand, within yhdYat 419.126 kb on - strand, within yhdYat 419.176 kb on + strand, within yhdYat 419.255 kb on + strand, within yhdYat 419.275 kb on - strand, within yhdYat 419.298 kb on + strand, within yhdYat 419.380 kb on - strand, within yhdYat 419.417 kb on - strandat 419.567 kb on + strandat 419.675 kb on - strand, within yhdZat 419.675 kb on - strand, within yhdZat 419.677 kb on + strand, within yhdZat 419.731 kb on + strand, within yhdZat 419.731 kb on + strand, within yhdZat 419.749 kb on + strand, within yhdZat 419.895 kb on - strand, within yhdZat 419.929 kb on + strand, within yhdZat 419.993 kb on - strand, within yhdZat 420.112 kb on - strand, within yhdZat 420.161 kb on - strand, within yhdZat 420.217 kb on + strandat 420.217 kb on + strandat 420.229 kb on + strandat 420.229 kb on + strandat 420.261 kb on - strandat 420.261 kb on - strandat 420.271 kb on + strandat 420.272 kb on - strandat 420.445 kb on + strandat 420.478 kb on + strandat 420.479 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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418,653 + yhdY BNILDI_02105 0.24 +0.7
418,653 + yhdY BNILDI_02105 0.24 -1.6
418,654 - yhdY BNILDI_02105 0.24 +0.8
418,654 - yhdY BNILDI_02105 0.24 +0.0
418,658 - yhdY BNILDI_02105 0.24 -0.8
418,692 + yhdY BNILDI_02105 0.27 -0.5
418,765 + yhdY BNILDI_02105 0.34 +0.2
418,767 + yhdY BNILDI_02105 0.34 +0.2
418,768 - yhdY BNILDI_02105 0.34 -0.1
418,817 - yhdY BNILDI_02105 0.38 -0.7
418,817 - yhdY BNILDI_02105 0.38 -0.1
418,875 - yhdY BNILDI_02105 0.44 -1.9
418,884 + yhdY BNILDI_02105 0.44 -1.1
418,884 + yhdY BNILDI_02105 0.44 +0.6
418,968 + yhdY BNILDI_02105 0.52 +1.0
419,022 - yhdY BNILDI_02105 0.57 -0.6
419,029 - yhdY BNILDI_02105 0.58 +2.3
419,029 - yhdY BNILDI_02105 0.58 +0.0
419,125 + yhdY BNILDI_02105 0.66 +0.8
419,125 + yhdY BNILDI_02105 0.66 +0.0
419,126 - yhdY BNILDI_02105 0.66 -0.8
419,126 - yhdY BNILDI_02105 0.66 -0.9
419,126 - yhdY BNILDI_02105 0.66 +0.2
419,126 - yhdY BNILDI_02105 0.66 +0.2
419,176 + yhdY BNILDI_02105 0.71 +1.3
419,255 + yhdY BNILDI_02105 0.78 +0.8
419,275 - yhdY BNILDI_02105 0.80 +0.1
419,298 + yhdY BNILDI_02105 0.82 -0.1
419,380 - yhdY BNILDI_02105 0.89 -0.5
419,417 - +1.2
419,567 + -1.6
419,675 - yhdZ BNILDI_02110 0.23 -0.2
419,675 - yhdZ BNILDI_02110 0.23 -0.2
419,677 + yhdZ BNILDI_02110 0.23 +0.6
419,731 + yhdZ BNILDI_02110 0.30 +1.8
419,731 + yhdZ BNILDI_02110 0.30 -1.2
419,749 + yhdZ BNILDI_02110 0.32 +0.3
419,895 - yhdZ BNILDI_02110 0.52 +0.0
419,929 + yhdZ BNILDI_02110 0.56 +2.3
419,993 - yhdZ BNILDI_02110 0.64 -0.3
420,112 - yhdZ BNILDI_02110 0.80 -1.1
420,161 - yhdZ BNILDI_02110 0.87 +0.0
420,217 + -0.4
420,217 + +1.3
420,229 + +0.4
420,229 + +1.2
420,261 - +1.3
420,261 - +0.8
420,271 + -1.3
420,272 - +0.0
420,445 + -0.8
420,478 + +0.6
420,479 - -0.4

Or see this region's nucleotide sequence