Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepD and prfH are separated by 56 nucleotides prfH and rtcB overlap by 4 nucleotides rtcB and yhbS are separated by 245 nucleotides yhbS and dinB are separated by 74 nucleotides
BNILDI_00270: pepD - cytosol nonspecific dipeptidase, at 59,219 to 60,676
pepD
BNILDI_00275: prfH - peptide chain release factor H, at 60,733 to 61,347
prfH
BNILDI_00280: rtcB - RNA ligase RtcB family protein, at 61,344 to 62,483
rtcB
BNILDI_00285: yhbS - GNAT family N-acetyltransferase, at 62,729 to 63,103
yhbS
BNILDI_00290: dinB - DNA polymerase IV, at 63,178 to 64,233
dinB
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1
2 at 60.372 kb on + strand, within pepD at 60.373 kb on - strand, within pepD at 60.405 kb on - strand, within pepD at 60.451 kb on - strand, within pepD at 60.462 kb on - strand, within pepD at 60.630 kb on - strand at 60.675 kb on - strand at 60.675 kb on - strand at 60.679 kb on - strand at 60.904 kb on - strand, within prfH at 60.973 kb on - strand, within prfH at 61.128 kb on + strand, within prfH at 61.129 kb on - strand, within prfH at 61.270 kb on + strand, within prfH at 61.411 kb on - strand at 61.411 kb on - strand at 61.455 kb on - strand at 61.455 kb on - strand at 61.589 kb on + strand, within rtcB at 61.635 kb on - strand, within rtcB at 61.645 kb on - strand, within rtcB at 61.657 kb on + strand, within rtcB at 61.658 kb on - strand, within rtcB at 61.658 kb on - strand, within rtcB at 61.680 kb on - strand, within rtcB at 61.815 kb on + strand, within rtcB at 61.844 kb on + strand, within rtcB at 61.881 kb on - strand, within rtcB at 61.882 kb on + strand, within rtcB at 61.882 kb on + strand, within rtcB at 61.883 kb on - strand, within rtcB at 61.948 kb on - strand, within rtcB at 61.979 kb on + strand, within rtcB at 62.213 kb on - strand, within rtcB at 62.228 kb on + strand, within rtcB at 62.341 kb on - strand, within rtcB at 62.359 kb on - strand, within rtcB at 62.445 kb on - strand at 62.499 kb on + strand at 62.500 kb on - strand at 62.698 kb on - strand at 62.878 kb on + strand, within yhbS at 62.879 kb on - strand, within yhbS at 62.951 kb on - strand, within yhbS at 62.952 kb on + strand, within yhbS at 63.085 kb on - strand at 63.132 kb on - strand at 63.156 kb on + strand at 63.170 kb on + strand at 63.298 kb on + strand, within dinB at 63.364 kb on + strand, within dinB at 63.477 kb on + strand, within dinB
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 60,372 + pepD BNILDI_00270 0.79 -0.1 60,373 - pepD BNILDI_00270 0.79 +2.5 60,405 - pepD BNILDI_00270 0.81 -0.8 60,451 - pepD BNILDI_00270 0.84 +0.3 60,462 - pepD BNILDI_00270 0.85 +0.3 60,630 - +0.8 60,675 - -0.5 60,675 - -0.2 60,679 - -0.0 60,904 - prfH BNILDI_00275 0.28 -0.3 60,973 - prfH BNILDI_00275 0.39 -0.2 61,128 + prfH BNILDI_00275 0.64 -2.0 61,129 - prfH BNILDI_00275 0.64 +0.3 61,270 + prfH BNILDI_00275 0.87 -0.8 61,411 - -1.3 61,411 - -0.6 61,455 - +2.0 61,455 - -0.1 61,589 + rtcB BNILDI_00280 0.21 -1.9 61,635 - rtcB BNILDI_00280 0.26 -2.0 61,645 - rtcB BNILDI_00280 0.26 -1.4 61,657 + rtcB BNILDI_00280 0.27 -0.0 61,658 - rtcB BNILDI_00280 0.28 +0.1 61,658 - rtcB BNILDI_00280 0.28 -1.4 61,680 - rtcB BNILDI_00280 0.29 -0.8 61,815 + rtcB BNILDI_00280 0.41 -0.8 61,844 + rtcB BNILDI_00280 0.44 -0.3 61,881 - rtcB BNILDI_00280 0.47 -0.9 61,882 + rtcB BNILDI_00280 0.47 -1.6 61,882 + rtcB BNILDI_00280 0.47 -0.2 61,883 - rtcB BNILDI_00280 0.47 -0.4 61,948 - rtcB BNILDI_00280 0.53 -0.0 61,979 + rtcB BNILDI_00280 0.56 -0.5 62,213 - rtcB BNILDI_00280 0.76 +0.7 62,228 + rtcB BNILDI_00280 0.78 +1.8 62,341 - rtcB BNILDI_00280 0.87 -0.4 62,359 - rtcB BNILDI_00280 0.89 +0.5 62,445 - -0.6 62,499 + +0.6 62,500 - +1.6 62,698 - +0.3 62,878 + yhbS BNILDI_00285 0.40 -1.3 62,879 - yhbS BNILDI_00285 0.40 -0.7 62,951 - yhbS BNILDI_00285 0.59 +0.4 62,952 + yhbS BNILDI_00285 0.59 +0.9 63,085 - -0.4 63,132 - -1.9 63,156 + +1.8 63,170 + -1.7 63,298 + dinB BNILDI_00290 0.11 -1.1 63,364 + dinB BNILDI_00290 0.18 -1.8 63,477 + dinB BNILDI_00290 0.28 +0.8
Or see this region's nucleotide sequence