Strain Fitness in Escherichia coli ECRC62 around BNILDI_22780

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiI and xseB are separated by 205 nucleotidesxseB and ispA overlap by 1 nucleotidesispA and dxs are separated by 24 nucleotides BNILDI_22770: thiI - tRNA sulfurtransferase, at 4,581,982 to 4,583,430 thiI BNILDI_22775: xseB - exodeoxyribonuclease VII small subunit, at 4,583,636 to 4,583,878 xseB BNILDI_22780: ispA - (2E,6E)-farnesyl diphosphate synthase, at 4,583,878 to 4,584,777 ispA BNILDI_22785: dxs - 1-deoxy-D-xylulose-5-phosphate synthase, at 4,584,802 to 4,586,664 dxs Position (kb) 4583 4584 4585Strain fitness (log2 ratio) -2 -1 0 1at 4583.026 kb on - strand, within thiIat 4583.036 kb on - strand, within thiIat 4583.051 kb on + strand, within thiIat 4583.321 kb on + strandat 4583.322 kb on - strandat 4583.330 kb on - strandat 4583.421 kb on + strandat 4583.468 kb on + strandat 4583.468 kb on + strandat 4583.468 kb on + strandat 4583.468 kb on + strandat 4583.480 kb on + strandat 4583.522 kb on + strandat 4583.523 kb on - strandat 4583.528 kb on + strandat 4583.556 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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4,583,026 - thiI BNILDI_22770 0.72 +0.9
4,583,036 - thiI BNILDI_22770 0.73 -1.8
4,583,051 + thiI BNILDI_22770 0.74 -0.2
4,583,321 + +0.2
4,583,322 - +0.9
4,583,330 - -0.2
4,583,421 + +1.5
4,583,468 + -1.1
4,583,468 + +0.2
4,583,468 + -0.7
4,583,468 + -2.3
4,583,480 + +0.9
4,583,522 + -0.8
4,583,523 - +0.5
4,583,528 + +0.5
4,583,556 + +1.4

Or see this region's nucleotide sequence