Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mntR and mntS are separated by 185 nucleotides mntS and opgE are separated by 271 nucleotides opgE and BNILDI_20820 are separated by 48 nucleotides BNILDI_20820 and yliM are separated by 52 nucleotides yliM and rhtA are separated by 234 nucleotides
BNILDI_20805: mntR - manganese-binding transcriptional regulator MntR, at 4,190,833 to 4,191,300
mntR
BNILDI_20810: mntS - manganase accumulation protein MntS, at 4,191,486 to 4,191,614
mntS
BNILDI_20815: opgE - phosphoethanolamine transferase, at 4,191,886 to 4,193,469
opgE
BNILDI_20820: BNILDI_20820 - Outer membrane protein X, at 4,193,518 to 4,194,033
_20820
BNILDI_20825: yliM - Protein YliM, at 4,194,086 to 4,194,151
yliM
BNILDI_20830: rhtA - threonine/homoserine exporter RhtA, at 4,194,386 to 4,195,273
rhtA
Position (kb)
4191
4192
4193
4194 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4190.969 kb on - strand, within mntR at 4191.179 kb on + strand, within mntR at 4191.179 kb on + strand, within mntR at 4191.190 kb on - strand, within mntR at 4191.327 kb on - strand at 4191.327 kb on - strand at 4191.327 kb on - strand at 4191.327 kb on - strand at 4191.331 kb on - strand at 4191.331 kb on - strand at 4191.331 kb on - strand at 4191.400 kb on + strand at 4191.409 kb on - strand at 4191.425 kb on + strand at 4191.426 kb on - strand at 4191.435 kb on + strand at 4191.435 kb on + strand at 4191.506 kb on + strand, within mntS at 4191.506 kb on + strand, within mntS at 4191.507 kb on - strand, within mntS at 4191.507 kb on - strand, within mntS at 4191.533 kb on + strand, within mntS at 4191.533 kb on + strand, within mntS at 4191.558 kb on - strand, within mntS at 4191.558 kb on - strand, within mntS at 4191.558 kb on - strand, within mntS at 4191.558 kb on - strand, within mntS at 4191.558 kb on - strand, within mntS at 4191.778 kb on + strand at 4191.780 kb on + strand at 4191.854 kb on + strand at 4191.873 kb on - strand at 4191.968 kb on + strand at 4191.971 kb on + strand at 4191.972 kb on - strand at 4192.149 kb on + strand, within opgE at 4192.217 kb on - strand, within opgE at 4192.222 kb on + strand, within opgE at 4192.223 kb on - strand, within opgE at 4192.235 kb on + strand, within opgE at 4192.325 kb on + strand, within opgE at 4192.383 kb on - strand, within opgE at 4192.386 kb on + strand, within opgE at 4192.386 kb on + strand, within opgE at 4192.531 kb on + strand, within opgE at 4192.540 kb on + strand, within opgE at 4192.540 kb on + strand, within opgE at 4192.541 kb on - strand, within opgE at 4192.640 kb on + strand, within opgE at 4192.651 kb on + strand, within opgE at 4192.652 kb on - strand, within opgE at 4192.652 kb on - strand, within opgE at 4192.808 kb on - strand, within opgE at 4192.852 kb on + strand, within opgE at 4192.852 kb on + strand, within opgE at 4192.852 kb on + strand, within opgE at 4192.853 kb on - strand, within opgE at 4192.968 kb on - strand, within opgE at 4193.018 kb on - strand, within opgE at 4193.018 kb on - strand, within opgE at 4193.084 kb on + strand, within opgE at 4193.099 kb on - strand, within opgE at 4193.191 kb on + strand, within opgE at 4193.192 kb on - strand, within opgE at 4193.208 kb on + strand, within opgE at 4193.208 kb on + strand, within opgE at 4193.209 kb on - strand, within opgE at 4193.209 kb on - strand, within opgE at 4193.370 kb on - strand at 4193.396 kb on - strand at 4193.396 kb on - strand at 4193.396 kb on - strand at 4193.415 kb on - strand at 4193.429 kb on - strand at 4193.520 kb on - strand at 4193.520 kb on - strand at 4193.554 kb on + strand at 4193.555 kb on - strand at 4193.555 kb on - strand at 4193.555 kb on - strand at 4193.565 kb on + strand at 4193.586 kb on - strand, within BNILDI_20820 at 4193.727 kb on - strand, within BNILDI_20820 at 4193.795 kb on + strand, within BNILDI_20820 at 4193.796 kb on - strand, within BNILDI_20820 at 4193.814 kb on + strand, within BNILDI_20820 at 4193.831 kb on + strand, within BNILDI_20820 at 4193.832 kb on - strand, within BNILDI_20820 at 4193.969 kb on - strand, within BNILDI_20820 at 4194.058 kb on - strand at 4194.076 kb on - strand at 4194.237 kb on - strand at 4194.254 kb on + strand at 4194.333 kb on - strand at 4194.333 kb on - strand at 4194.355 kb on + strand at 4194.359 kb on + strand at 4194.365 kb on + strand at 4194.365 kb on + strand at 4194.403 kb on + strand at 4194.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 4,190,969 - mntR BNILDI_20805 0.29 +2.8 4,191,179 + mntR BNILDI_20805 0.74 -1.4 4,191,179 + mntR BNILDI_20805 0.74 +0.7 4,191,190 - mntR BNILDI_20805 0.76 +0.8 4,191,327 - -1.6 4,191,327 - +1.5 4,191,327 - -0.4 4,191,327 - -0.0 4,191,331 - -1.6 4,191,331 - -1.4 4,191,331 - +1.3 4,191,400 + -0.2 4,191,409 - -1.2 4,191,425 + -3.0 4,191,426 - +1.0 4,191,435 + -2.3 4,191,435 + -1.6 4,191,506 + mntS BNILDI_20810 0.16 +0.1 4,191,506 + mntS BNILDI_20810 0.16 -0.7 4,191,507 - mntS BNILDI_20810 0.16 -1.9 4,191,507 - mntS BNILDI_20810 0.16 +0.8 4,191,533 + mntS BNILDI_20810 0.36 +0.8 4,191,533 + mntS BNILDI_20810 0.36 -0.3 4,191,558 - mntS BNILDI_20810 0.56 -2.0 4,191,558 - mntS BNILDI_20810 0.56 +0.3 4,191,558 - mntS BNILDI_20810 0.56 -0.5 4,191,558 - mntS BNILDI_20810 0.56 +2.8 4,191,558 - mntS BNILDI_20810 0.56 +0.5 4,191,778 + +0.3 4,191,780 + -0.1 4,191,854 + +0.8 4,191,873 - -1.7 4,191,968 + -1.3 4,191,971 + -0.8 4,191,972 - +0.5 4,192,149 + opgE BNILDI_20815 0.17 -0.0 4,192,217 - opgE BNILDI_20815 0.21 +1.8 4,192,222 + opgE BNILDI_20815 0.21 +0.0 4,192,223 - opgE BNILDI_20815 0.21 +0.7 4,192,235 + opgE BNILDI_20815 0.22 -0.2 4,192,325 + opgE BNILDI_20815 0.28 -0.7 4,192,383 - opgE BNILDI_20815 0.31 +3.4 4,192,386 + opgE BNILDI_20815 0.32 -1.1 4,192,386 + opgE BNILDI_20815 0.32 +2.2 4,192,531 + opgE BNILDI_20815 0.41 -0.8 4,192,540 + opgE BNILDI_20815 0.41 -1.5 4,192,540 + opgE BNILDI_20815 0.41 -0.1 4,192,541 - opgE BNILDI_20815 0.41 -1.6 4,192,640 + opgE BNILDI_20815 0.48 +1.2 4,192,651 + opgE BNILDI_20815 0.48 -0.4 4,192,652 - opgE BNILDI_20815 0.48 -0.3 4,192,652 - opgE BNILDI_20815 0.48 -1.5 4,192,808 - opgE BNILDI_20815 0.58 +0.8 4,192,852 + opgE BNILDI_20815 0.61 +0.5 4,192,852 + opgE BNILDI_20815 0.61 -1.0 4,192,852 + opgE BNILDI_20815 0.61 -0.4 4,192,853 - opgE BNILDI_20815 0.61 +1.4 4,192,968 - opgE BNILDI_20815 0.68 +1.8 4,193,018 - opgE BNILDI_20815 0.71 -1.8 4,193,018 - opgE BNILDI_20815 0.71 +1.2 4,193,084 + opgE BNILDI_20815 0.76 +0.2 4,193,099 - opgE BNILDI_20815 0.77 -1.8 4,193,191 + opgE BNILDI_20815 0.82 +0.2 4,193,192 - opgE BNILDI_20815 0.82 -0.9 4,193,208 + opgE BNILDI_20815 0.83 +1.8 4,193,208 + opgE BNILDI_20815 0.83 -1.4 4,193,209 - opgE BNILDI_20815 0.84 -1.7 4,193,209 - opgE BNILDI_20815 0.84 +0.4 4,193,370 - -0.2 4,193,396 - +0.4 4,193,396 - -1.3 4,193,396 - -0.6 4,193,415 - -1.1 4,193,429 - -2.0 4,193,520 - -1.5 4,193,520 - -0.2 4,193,554 + +0.2 4,193,555 - +0.5 4,193,555 - -0.3 4,193,555 - +1.0 4,193,565 + -0.1 4,193,586 - BNILDI_20820 0.13 +0.4 4,193,727 - BNILDI_20820 0.41 -0.8 4,193,795 + BNILDI_20820 0.54 +0.8 4,193,796 - BNILDI_20820 0.54 -0.2 4,193,814 + BNILDI_20820 0.57 +1.0 4,193,831 + BNILDI_20820 0.61 +0.2 4,193,832 - BNILDI_20820 0.61 -1.0 4,193,969 - BNILDI_20820 0.87 +1.2 4,194,058 - -1.5 4,194,076 - -0.9 4,194,237 - -1.0 4,194,254 + -0.0 4,194,333 - -1.2 4,194,333 - +1.3 4,194,355 + +2.8 4,194,359 + +0.7 4,194,365 + +1.8 4,194,365 + -0.0 4,194,403 + +1.8 4,194,433 - -0.4
Or see this region's nucleotide sequence