Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tam and lsrG are separated by 56 nucleotides lsrG and lsrF are separated by 23 nucleotides lsrF and lsrB are separated by 26 nucleotides
BNILDI_17270: tam - trans-aconitate 2-methyltransferase, at 3,459,506 to 3,460,264
tam
BNILDI_17275: lsrG - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase, at 3,460,321 to 3,460,611
lsrG
BNILDI_17280: lsrF - 3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase, at 3,460,635 to 3,461,510
lsrF
BNILDI_17285: lsrB - autoinducer 2 ABC transporter substrate-binding protein LsrB, at 3,461,537 to 3,462,559
lsrB
Position (kb)
3460
3461
3462 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3459.715 kb on - strand, within tam at 3459.717 kb on + strand, within tam at 3459.718 kb on - strand, within tam at 3459.764 kb on - strand, within tam at 3459.768 kb on + strand, within tam at 3459.772 kb on - strand, within tam at 3459.905 kb on - strand, within tam at 3460.164 kb on + strand, within tam at 3460.239 kb on + strand at 3460.239 kb on + strand at 3460.241 kb on + strand at 3460.242 kb on - strand at 3460.270 kb on + strand at 3460.271 kb on - strand at 3460.299 kb on - strand at 3460.444 kb on - strand, within lsrG at 3460.458 kb on + strand, within lsrG at 3460.461 kb on - strand, within lsrG at 3460.611 kb on + strand at 3460.612 kb on - strand at 3460.655 kb on - strand at 3460.746 kb on - strand, within lsrF at 3460.895 kb on - strand, within lsrF at 3460.897 kb on + strand, within lsrF at 3461.084 kb on - strand, within lsrF at 3461.259 kb on + strand, within lsrF at 3461.259 kb on + strand, within lsrF at 3461.294 kb on + strand, within lsrF at 3461.294 kb on + strand, within lsrF at 3461.333 kb on - strand, within lsrF at 3461.500 kb on - strand at 3461.525 kb on - strand at 3461.595 kb on - strand at 3461.620 kb on - strand at 3461.730 kb on + strand, within lsrB at 3461.730 kb on + strand, within lsrB at 3461.799 kb on + strand, within lsrB at 3461.800 kb on - strand, within lsrB at 3461.928 kb on + strand, within lsrB at 3461.928 kb on + strand, within lsrB at 3461.929 kb on - strand, within lsrB at 3461.940 kb on - strand, within lsrB at 3461.957 kb on + strand, within lsrB at 3462.113 kb on - strand, within lsrB at 3462.134 kb on - strand, within lsrB at 3462.145 kb on + strand, within lsrB at 3462.145 kb on + strand, within lsrB at 3462.176 kb on - strand, within lsrB at 3462.176 kb on - strand, within lsrB at 3462.202 kb on + strand, within lsrB at 3462.307 kb on - strand, within lsrB at 3462.338 kb on + strand, within lsrB at 3462.339 kb on - strand, within lsrB at 3462.473 kb on - strand at 3462.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 3,459,715 - tam BNILDI_17270 0.28 -0.1 3,459,717 + tam BNILDI_17270 0.28 +1.3 3,459,718 - tam BNILDI_17270 0.28 -1.5 3,459,764 - tam BNILDI_17270 0.34 +0.2 3,459,768 + tam BNILDI_17270 0.35 +1.2 3,459,772 - tam BNILDI_17270 0.35 +0.6 3,459,905 - tam BNILDI_17270 0.53 +2.8 3,460,164 + tam BNILDI_17270 0.87 +0.4 3,460,239 + -1.2 3,460,239 + +1.4 3,460,241 + +0.3 3,460,242 - -0.0 3,460,270 + +2.2 3,460,271 - -0.9 3,460,299 - -1.1 3,460,444 - lsrG BNILDI_17275 0.42 -0.8 3,460,458 + lsrG BNILDI_17275 0.47 -0.0 3,460,461 - lsrG BNILDI_17275 0.48 +0.6 3,460,611 + -0.0 3,460,612 - +2.8 3,460,655 - -0.1 3,460,746 - lsrF BNILDI_17280 0.13 -1.5 3,460,895 - lsrF BNILDI_17280 0.30 -1.3 3,460,897 + lsrF BNILDI_17280 0.30 -1.0 3,461,084 - lsrF BNILDI_17280 0.51 -0.7 3,461,259 + lsrF BNILDI_17280 0.71 +2.3 3,461,259 + lsrF BNILDI_17280 0.71 -1.4 3,461,294 + lsrF BNILDI_17280 0.75 -0.8 3,461,294 + lsrF BNILDI_17280 0.75 +2.8 3,461,333 - lsrF BNILDI_17280 0.80 -2.4 3,461,500 - -0.0 3,461,525 - +0.3 3,461,595 - +0.8 3,461,620 - -0.2 3,461,730 + lsrB BNILDI_17285 0.19 +0.8 3,461,730 + lsrB BNILDI_17285 0.19 +0.5 3,461,799 + lsrB BNILDI_17285 0.26 -0.4 3,461,800 - lsrB BNILDI_17285 0.26 +1.2 3,461,928 + lsrB BNILDI_17285 0.38 -1.0 3,461,928 + lsrB BNILDI_17285 0.38 -0.3 3,461,929 - lsrB BNILDI_17285 0.38 +2.4 3,461,940 - lsrB BNILDI_17285 0.39 +0.2 3,461,957 + lsrB BNILDI_17285 0.41 -0.4 3,462,113 - lsrB BNILDI_17285 0.56 +1.2 3,462,134 - lsrB BNILDI_17285 0.58 -0.1 3,462,145 + lsrB BNILDI_17285 0.59 -1.4 3,462,145 + lsrB BNILDI_17285 0.59 -0.0 3,462,176 - lsrB BNILDI_17285 0.62 +1.5 3,462,176 - lsrB BNILDI_17285 0.62 -1.6 3,462,202 + lsrB BNILDI_17285 0.65 -0.0 3,462,307 - lsrB BNILDI_17285 0.75 -0.9 3,462,338 + lsrB BNILDI_17285 0.78 +1.8 3,462,339 - lsrB BNILDI_17285 0.78 +0.1 3,462,473 - +3.1 3,462,497 - -0.0
Or see this region's nucleotide sequence