Strain Fitness in Escherichia coli ECRC62 around BNILDI_16235

Experiment: RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspy and ves are separated by 202 nucleotidesves and cho overlap by 41 nucleotides BNILDI_16230: spy - ATP-independent periplasmic protein-refolding chaperone Spy, at 3,256,392 to 3,256,877 spy BNILDI_16235: ves - environmental stress-induced protein Ves, at 3,257,080 to 3,257,655 ves BNILDI_16240: cho - excinuclease Cho, at 3,257,615 to 3,258,502 cho Position (kb) 3257 3258Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3256.806 kb on - strand, within spyat 3256.854 kb on - strandat 3256.919 kb on - strandat 3257.004 kb on + strandat 3257.005 kb on - strandat 3257.038 kb on + strandat 3257.038 kb on + strandat 3257.253 kb on - strand, within vesat 3257.305 kb on - strand, within vesat 3257.310 kb on + strand, within vesat 3257.311 kb on - strand, within vesat 3257.357 kb on + strand, within vesat 3257.401 kb on - strand, within vesat 3257.452 kb on - strand, within vesat 3257.458 kb on - strand, within vesat 3257.460 kb on + strand, within vesat 3257.788 kb on + strand, within choat 3257.791 kb on + strand, within choat 3257.792 kb on - strand, within choat 3257.792 kb on - strand, within choat 3257.979 kb on - strand, within choat 3258.140 kb on + strand, within choat 3258.209 kb on + strand, within choat 3258.309 kb on - strand, within choat 3258.354 kb on + strand, within choat 3258.355 kb on - strand, within choat 3258.410 kb on + strand, within choat 3258.431 kb on + strandat 3258.432 kb on - strandat 3258.432 kb on - strandat 3258.632 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RB68
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3,256,806 - spy BNILDI_16230 0.85 +0.4
3,256,854 - -0.7
3,256,919 - +1.4
3,257,004 + +0.6
3,257,005 - +1.3
3,257,038 + +2.0
3,257,038 + +0.2
3,257,253 - ves BNILDI_16235 0.30 +2.3
3,257,305 - ves BNILDI_16235 0.39 +0.7
3,257,310 + ves BNILDI_16235 0.40 +1.4
3,257,311 - ves BNILDI_16235 0.40 +1.2
3,257,357 + ves BNILDI_16235 0.48 -0.1
3,257,401 - ves BNILDI_16235 0.56 +1.0
3,257,452 - ves BNILDI_16235 0.65 +0.2
3,257,458 - ves BNILDI_16235 0.66 -0.5
3,257,460 + ves BNILDI_16235 0.66 +0.2
3,257,788 + cho BNILDI_16240 0.19 +3.0
3,257,791 + cho BNILDI_16240 0.20 -0.7
3,257,792 - cho BNILDI_16240 0.20 +0.6
3,257,792 - cho BNILDI_16240 0.20 +0.3
3,257,979 - cho BNILDI_16240 0.41 -1.1
3,258,140 + cho BNILDI_16240 0.59 -0.1
3,258,209 + cho BNILDI_16240 0.67 -0.9
3,258,309 - cho BNILDI_16240 0.78 +0.5
3,258,354 + cho BNILDI_16240 0.83 +1.5
3,258,355 - cho BNILDI_16240 0.83 +2.6
3,258,410 + cho BNILDI_16240 0.90 -0.1
3,258,431 + -0.7
3,258,432 - -0.1
3,258,432 - +1.0
3,258,632 - -2.1

Or see this region's nucleotide sequence