Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folX and yfcG are separated by 56 nucleotides yfcG and yfcF are separated by 135 nucleotides yfcF and yfcE are separated by 55 nucleotides yfcE and yfcD are separated by 57 nucleotides
BNILDI_13300: folX - dihydroneopterin triphosphate 2'-epimerase, at 2,663,378 to 2,663,740
folX
BNILDI_13305: yfcG - GSH-dependent disulfide bond oxidoreductase, at 2,663,797 to 2,664,444
yfcG
BNILDI_13310: yfcF - glutathione transferase, at 2,664,580 to 2,665,224
yfcF
BNILDI_13315: yfcE - phosphodiesterase, at 2,665,280 to 2,665,831
yfcE
BNILDI_13320: yfcD - NUDIX hydrolase YfcD, at 2,665,889 to 2,666,431
yfcD
Position (kb)
2664
2665
2666 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2663.641 kb on + strand, within folX at 2663.693 kb on - strand, within folX at 2663.711 kb on + strand at 2663.760 kb on + strand at 2663.761 kb on - strand at 2663.765 kb on - strand at 2663.765 kb on - strand at 2663.765 kb on - strand at 2663.774 kb on + strand at 2663.775 kb on - strand at 2663.798 kb on + strand at 2663.799 kb on - strand at 2663.897 kb on + strand, within yfcG at 2663.898 kb on - strand, within yfcG at 2663.898 kb on - strand, within yfcG at 2663.935 kb on - strand, within yfcG at 2663.969 kb on + strand, within yfcG at 2664.131 kb on - strand, within yfcG at 2664.212 kb on + strand, within yfcG at 2664.352 kb on + strand, within yfcG at 2664.431 kb on + strand at 2664.461 kb on + strand at 2664.462 kb on - strand at 2664.482 kb on + strand at 2664.510 kb on - strand at 2664.541 kb on - strand at 2664.542 kb on + strand at 2664.543 kb on - strand at 2664.543 kb on - strand at 2664.579 kb on - strand at 2664.631 kb on + strand at 2664.631 kb on + strand at 2664.632 kb on - strand at 2664.632 kb on - strand at 2664.765 kb on - strand, within yfcF at 2664.849 kb on + strand, within yfcF at 2664.853 kb on + strand, within yfcF at 2665.058 kb on + strand, within yfcF at 2665.059 kb on - strand, within yfcF at 2665.083 kb on + strand, within yfcF at 2665.104 kb on + strand, within yfcF at 2665.118 kb on + strand, within yfcF at 2665.119 kb on - strand, within yfcF at 2665.194 kb on + strand at 2665.249 kb on + strand at 2665.249 kb on + strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2665.250 kb on - strand at 2665.471 kb on + strand, within yfcE at 2665.471 kb on + strand, within yfcE at 2665.471 kb on + strand, within yfcE at 2665.471 kb on + strand, within yfcE at 2665.471 kb on + strand, within yfcE at 2665.472 kb on - strand, within yfcE at 2665.472 kb on - strand, within yfcE at 2665.472 kb on - strand, within yfcE at 2665.472 kb on - strand, within yfcE at 2665.472 kb on - strand, within yfcE at 2665.557 kb on + strand, within yfcE at 2665.557 kb on + strand, within yfcE at 2665.558 kb on - strand, within yfcE at 2665.561 kb on - strand, within yfcE at 2665.649 kb on + strand, within yfcE at 2665.650 kb on - strand, within yfcE at 2665.770 kb on + strand, within yfcE at 2665.770 kb on + strand, within yfcE at 2665.801 kb on + strand at 2665.802 kb on - strand at 2665.830 kb on - strand at 2665.912 kb on + strand at 2665.913 kb on - strand at 2665.927 kb on + strand at 2666.089 kb on - strand, within yfcD
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 2,663,641 + folX BNILDI_13300 0.72 -0.4 2,663,693 - folX BNILDI_13300 0.87 +1.7 2,663,711 + -2.3 2,663,760 + -1.4 2,663,761 - -0.6 2,663,765 - -1.3 2,663,765 - +1.0 2,663,765 - -0.1 2,663,774 + +0.2 2,663,775 - -1.5 2,663,798 + +2.7 2,663,799 - -1.5 2,663,897 + yfcG BNILDI_13305 0.15 -0.7 2,663,898 - yfcG BNILDI_13305 0.16 -0.9 2,663,898 - yfcG BNILDI_13305 0.16 -1.4 2,663,935 - yfcG BNILDI_13305 0.21 -0.4 2,663,969 + yfcG BNILDI_13305 0.27 +0.5 2,664,131 - yfcG BNILDI_13305 0.52 -1.3 2,664,212 + yfcG BNILDI_13305 0.64 -1.0 2,664,352 + yfcG BNILDI_13305 0.86 +0.7 2,664,431 + +1.7 2,664,461 + +0.2 2,664,462 - -0.3 2,664,482 + +1.7 2,664,510 - -2.2 2,664,541 - +1.2 2,664,542 + -0.7 2,664,543 - -0.4 2,664,543 - +1.7 2,664,579 - -1.4 2,664,631 + +0.5 2,664,631 + +0.1 2,664,632 - -0.4 2,664,632 - +0.3 2,664,765 - yfcF BNILDI_13310 0.29 +1.0 2,664,849 + yfcF BNILDI_13310 0.42 +0.4 2,664,853 + yfcF BNILDI_13310 0.42 -0.1 2,665,058 + yfcF BNILDI_13310 0.74 +2.2 2,665,059 - yfcF BNILDI_13310 0.74 -0.1 2,665,083 + yfcF BNILDI_13310 0.78 +0.2 2,665,104 + yfcF BNILDI_13310 0.81 +1.7 2,665,118 + yfcF BNILDI_13310 0.83 +4.1 2,665,119 - yfcF BNILDI_13310 0.84 -0.1 2,665,194 + -0.8 2,665,249 + -0.3 2,665,249 + +0.5 2,665,250 - -0.8 2,665,250 - -2.4 2,665,250 - +0.0 2,665,250 - -0.6 2,665,250 - -3.1 2,665,250 - -1.3 2,665,250 - +2.7 2,665,471 + yfcE BNILDI_13315 0.35 +1.1 2,665,471 + yfcE BNILDI_13315 0.35 +1.3 2,665,471 + yfcE BNILDI_13315 0.35 +1.0 2,665,471 + yfcE BNILDI_13315 0.35 +1.7 2,665,471 + yfcE BNILDI_13315 0.35 +0.2 2,665,472 - yfcE BNILDI_13315 0.35 +1.2 2,665,472 - yfcE BNILDI_13315 0.35 +2.7 2,665,472 - yfcE BNILDI_13315 0.35 -0.1 2,665,472 - yfcE BNILDI_13315 0.35 -2.5 2,665,472 - yfcE BNILDI_13315 0.35 +0.6 2,665,557 + yfcE BNILDI_13315 0.50 +2.7 2,665,557 + yfcE BNILDI_13315 0.50 -1.0 2,665,558 - yfcE BNILDI_13315 0.50 +0.3 2,665,561 - yfcE BNILDI_13315 0.51 -1.3 2,665,649 + yfcE BNILDI_13315 0.67 -0.8 2,665,650 - yfcE BNILDI_13315 0.67 -0.8 2,665,770 + yfcE BNILDI_13315 0.89 +1.6 2,665,770 + yfcE BNILDI_13315 0.89 -1.7 2,665,801 + +1.4 2,665,802 - +0.2 2,665,830 - +1.3 2,665,912 + -0.8 2,665,913 - -1.0 2,665,927 + -0.1 2,666,089 - yfcD BNILDI_13320 0.37 +2.7
Or see this region's nucleotide sequence