Experiment: RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yggM and hemW are separated by 241 nucleotides hemW and rdgB overlap by 8 nucleotides rdgB and yggU are separated by 7 nucleotides yggU and yggT overlap by 4 nucleotides
BNILDI_09670: yggM - Uncharacterized protein YggM, at 1,947,957 to 1,948,964
yggM
BNILDI_09685: hemW - radical SAM family heme chaperone HemW, at 1,949,206 to 1,950,342
hemW
BNILDI_09690: rdgB - XTP/dITP diphosphatase, at 1,950,335 to 1,950,928
rdgB
BNILDI_09695: yggU - DUF167 domain-containing protein, at 1,950,936 to 1,951,226
yggU
BNILDI_09700: yggT - osmotic shock tolerance protein YggT, at 1,951,223 to 1,951,789
yggT
Position (kb)
1949
1950
1951 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1948.238 kb on + strand, within yggM at 1948.239 kb on - strand, within yggM at 1948.239 kb on - strand, within yggM at 1948.370 kb on + strand, within yggM at 1948.371 kb on - strand, within yggM at 1948.374 kb on + strand, within yggM at 1948.374 kb on + strand, within yggM at 1948.412 kb on + strand, within yggM at 1948.574 kb on + strand, within yggM at 1948.638 kb on - strand, within yggM at 1948.642 kb on - strand, within yggM at 1948.682 kb on + strand, within yggM at 1948.701 kb on + strand, within yggM at 1948.702 kb on - strand, within yggM at 1948.702 kb on - strand, within yggM at 1948.788 kb on - strand, within yggM at 1948.958 kb on + strand at 1948.958 kb on + strand at 1948.958 kb on + strand at 1948.958 kb on + strand at 1948.963 kb on - strand at 1948.973 kb on + strand at 1948.973 kb on + strand at 1948.973 kb on + strand at 1948.973 kb on + strand at 1948.974 kb on - strand at 1948.974 kb on - strand at 1949.051 kb on - strand at 1949.137 kb on + strand at 1949.165 kb on - strand at 1949.565 kb on - strand, within hemW at 1949.637 kb on - strand, within hemW at 1949.663 kb on - strand, within hemW at 1949.684 kb on - strand, within hemW at 1949.739 kb on + strand, within hemW at 1949.740 kb on - strand, within hemW at 1949.743 kb on - strand, within hemW at 1949.804 kb on - strand, within hemW at 1949.869 kb on - strand, within hemW at 1949.869 kb on - strand, within hemW at 1949.976 kb on + strand, within hemW at 1950.054 kb on + strand, within hemW at 1950.166 kb on + strand, within hemW at 1950.167 kb on - strand, within hemW at 1950.186 kb on - strand, within hemW at 1950.314 kb on + strand at 1950.337 kb on - strand at 1950.515 kb on - strand, within rdgB at 1950.558 kb on + strand, within rdgB at 1950.559 kb on - strand, within rdgB at 1950.559 kb on - strand, within rdgB at 1950.560 kb on + strand, within rdgB at 1950.560 kb on + strand, within rdgB at 1950.560 kb on + strand, within rdgB at 1950.561 kb on - strand, within rdgB at 1950.678 kb on + strand, within rdgB at 1950.678 kb on + strand, within rdgB at 1950.679 kb on - strand, within rdgB at 1950.707 kb on + strand, within rdgB at 1950.933 kb on + strand at 1950.938 kb on - strand at 1950.938 kb on - strand at 1950.938 kb on - strand at 1950.938 kb on - strand at 1950.942 kb on - strand at 1951.124 kb on + strand, within yggU at 1951.125 kb on - strand, within yggU at 1951.140 kb on + strand, within yggU at 1951.180 kb on + strand, within yggU at 1951.180 kb on + strand, within yggU at 1951.180 kb on + strand, within yggU at 1951.208 kb on + strand at 1951.208 kb on + strand at 1951.208 kb on + strand at 1951.226 kb on + strand at 1951.271 kb on + strand at 1951.272 kb on - strand at 1951.275 kb on - strand at 1951.303 kb on - strand, within yggT at 1951.303 kb on - strand, within yggT
Per-strain Table
Position Strand Gene LocusTag Fraction RB68 remove 1,948,238 + yggM BNILDI_09670 0.28 -2.9 1,948,239 - yggM BNILDI_09670 0.28 +0.9 1,948,239 - yggM BNILDI_09670 0.28 -0.9 1,948,370 + yggM BNILDI_09670 0.41 +0.1 1,948,371 - yggM BNILDI_09670 0.41 +0.1 1,948,374 + yggM BNILDI_09670 0.41 -1.1 1,948,374 + yggM BNILDI_09670 0.41 -0.7 1,948,412 + yggM BNILDI_09670 0.45 +0.1 1,948,574 + yggM BNILDI_09670 0.61 +0.9 1,948,638 - yggM BNILDI_09670 0.68 +0.8 1,948,642 - yggM BNILDI_09670 0.68 -0.9 1,948,682 + yggM BNILDI_09670 0.72 +1.3 1,948,701 + yggM BNILDI_09670 0.74 +0.6 1,948,702 - yggM BNILDI_09670 0.74 -0.2 1,948,702 - yggM BNILDI_09670 0.74 -0.1 1,948,788 - yggM BNILDI_09670 0.82 +0.1 1,948,958 + -0.1 1,948,958 + +2.5 1,948,958 + +0.2 1,948,958 + +4.1 1,948,963 - -3.2 1,948,973 + +0.7 1,948,973 + +0.2 1,948,973 + +1.5 1,948,973 + -0.3 1,948,974 - +1.5 1,948,974 - -0.1 1,949,051 - -0.7 1,949,137 + +0.7 1,949,165 - -1.0 1,949,565 - hemW BNILDI_09685 0.32 +1.5 1,949,637 - hemW BNILDI_09685 0.38 +2.5 1,949,663 - hemW BNILDI_09685 0.40 -1.0 1,949,684 - hemW BNILDI_09685 0.42 -1.1 1,949,739 + hemW BNILDI_09685 0.47 -0.7 1,949,740 - hemW BNILDI_09685 0.47 -0.7 1,949,743 - hemW BNILDI_09685 0.47 -2.5 1,949,804 - hemW BNILDI_09685 0.53 +0.5 1,949,869 - hemW BNILDI_09685 0.58 -0.3 1,949,869 - hemW BNILDI_09685 0.58 -1.0 1,949,976 + hemW BNILDI_09685 0.68 +0.4 1,950,054 + hemW BNILDI_09685 0.75 -0.9 1,950,166 + hemW BNILDI_09685 0.84 +1.5 1,950,167 - hemW BNILDI_09685 0.85 -0.3 1,950,186 - hemW BNILDI_09685 0.86 -0.3 1,950,314 + +0.0 1,950,337 - +0.7 1,950,515 - rdgB BNILDI_09690 0.30 -2.1 1,950,558 + rdgB BNILDI_09690 0.38 +0.3 1,950,559 - rdgB BNILDI_09690 0.38 -1.7 1,950,559 - rdgB BNILDI_09690 0.38 -1.4 1,950,560 + rdgB BNILDI_09690 0.38 +1.5 1,950,560 + rdgB BNILDI_09690 0.38 -0.6 1,950,560 + rdgB BNILDI_09690 0.38 -1.0 1,950,561 - rdgB BNILDI_09690 0.38 +0.0 1,950,678 + rdgB BNILDI_09690 0.58 +1.2 1,950,678 + rdgB BNILDI_09690 0.58 +0.0 1,950,679 - rdgB BNILDI_09690 0.58 -2.4 1,950,707 + rdgB BNILDI_09690 0.63 -0.7 1,950,933 + -1.4 1,950,938 - +1.1 1,950,938 - -0.3 1,950,938 - +0.1 1,950,938 - +2.5 1,950,942 - -0.1 1,951,124 + yggU BNILDI_09695 0.65 +0.5 1,951,125 - yggU BNILDI_09695 0.65 +0.1 1,951,140 + yggU BNILDI_09695 0.70 +0.9 1,951,180 + yggU BNILDI_09695 0.84 -1.6 1,951,180 + yggU BNILDI_09695 0.84 +3.1 1,951,180 + yggU BNILDI_09695 0.84 -1.1 1,951,208 + +0.2 1,951,208 + +1.1 1,951,208 + +0.6 1,951,226 + -0.9 1,951,271 + -0.1 1,951,272 - +1.9 1,951,275 - -0.2 1,951,303 - yggT BNILDI_09700 0.14 +0.5 1,951,303 - yggT BNILDI_09700 0.14 +0.9
Or see this region's nucleotide sequence